Limits...
Id-1 is not expressed in the luminal epithelial cells of mammary glands.

Uehara N, Chou YC, Galvez JJ, de-Candia P, Cardiff RD, Benezra R, Shyamala G - Breast Cancer Res. (2003)

Bottom Line: In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected.In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells.Id-1 is not expressed in the luminal epithelial cells of mammary glands.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Life Sciences, Lawrence Berkeley National Laboratory, University of California Berkeley, USA.

ABSTRACT

Background: The family of inhibitor of differentiation/DNA binding (Id) proteins is known to regulate development in several tissues. One member of this gene family, Id-1, has been implicated in mammary development and carcinogenesis. Mammary glands contain various cell types, among which the luminal epithelial cells are primarily targeted for proliferation, differentiation and carcinogenesis. Therefore, to assess the precise significance of Id-1 in mammary biology and carcinogenesis, we examined its cellular localization in vivo using immunohistochemistry.

Methods: Extracts of whole mammary glands from wild type and Id-1 mutant mice, and tissue sections from paraffin-embedded mouse mammary glands from various developmental stages and normal human breast were subjected to immunoblot and immunohistochemical analyses, respectively. In both these procedures, an anti-Id-1 rabbit polyclonal antibody was used for detection of Id-1.

Results: In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected. In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells.

Conclusion: Id-1 is not expressed in the luminal epithelial cells of mammary glands.

Show MeSH

Related in: MedlinePlus

Immunohistochemical analyses for Id-1 in mouse mammary glands during various phases of development. Mammary glands from (A) early pregnant, (B) lactating, (C) lactational involuting and (D) pubertal mice were analyzed for the presence of Id-1 as described in the text. Note that, in all the mammary ducts, immunoreactivity is present only in myoepithelial cells and not in the luminal cells. Immunoreactivity is also not detected in the terminal end bud of the pubertal mouse. Original magnification, 400 ×.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC154143&req=5

Figure 4: Immunohistochemical analyses for Id-1 in mouse mammary glands during various phases of development. Mammary glands from (A) early pregnant, (B) lactating, (C) lactational involuting and (D) pubertal mice were analyzed for the presence of Id-1 as described in the text. Note that, in all the mammary ducts, immunoreactivity is present only in myoepithelial cells and not in the luminal cells. Immunoreactivity is also not detected in the terminal end bud of the pubertal mouse. Original magnification, 400 ×.

Mentions: It is well known that mammary epithelial cells of adult non-pregnant females, for the most part, are mitotically quiescent and proliferate extensively only with the onset of pregnancy [10]. Therefore, to examine whether the lack of expression of Id-1 in the luminal epithelial cells was related to its quiescent state, we also analyzed the tissues from early pregnant females. The patterns of immunostaining in mammary glands did not change either during pregnancy (Fig. 4A), during lactation (Fig. 4B) or during postlactational involution (Fig. 4C). As such, immunoreactivity was still confined to the cytoplasm of the myoepithelial cells and was not detected in luminal epithelial cells. Similar results were also obtained with tissues isolated from the Balb/c strain of mice (data not shown), the strain from which SCp2 cells had been derived [11]. In addition to pregnancy, mammary glands also proliferate extensively at the onset of puberty and, in these glands, the site of intense mitotic activity resides in specialized structures called the terminal end buds [12]. In terminal end buds of pubertal females, immunoreactivity was not detected either in the body cells or the cap cells (Fig. 4D) present at the tips.


Id-1 is not expressed in the luminal epithelial cells of mammary glands.

Uehara N, Chou YC, Galvez JJ, de-Candia P, Cardiff RD, Benezra R, Shyamala G - Breast Cancer Res. (2003)

Immunohistochemical analyses for Id-1 in mouse mammary glands during various phases of development. Mammary glands from (A) early pregnant, (B) lactating, (C) lactational involuting and (D) pubertal mice were analyzed for the presence of Id-1 as described in the text. Note that, in all the mammary ducts, immunoreactivity is present only in myoepithelial cells and not in the luminal cells. Immunoreactivity is also not detected in the terminal end bud of the pubertal mouse. Original magnification, 400 ×.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC154143&req=5

Figure 4: Immunohistochemical analyses for Id-1 in mouse mammary glands during various phases of development. Mammary glands from (A) early pregnant, (B) lactating, (C) lactational involuting and (D) pubertal mice were analyzed for the presence of Id-1 as described in the text. Note that, in all the mammary ducts, immunoreactivity is present only in myoepithelial cells and not in the luminal cells. Immunoreactivity is also not detected in the terminal end bud of the pubertal mouse. Original magnification, 400 ×.
Mentions: It is well known that mammary epithelial cells of adult non-pregnant females, for the most part, are mitotically quiescent and proliferate extensively only with the onset of pregnancy [10]. Therefore, to examine whether the lack of expression of Id-1 in the luminal epithelial cells was related to its quiescent state, we also analyzed the tissues from early pregnant females. The patterns of immunostaining in mammary glands did not change either during pregnancy (Fig. 4A), during lactation (Fig. 4B) or during postlactational involution (Fig. 4C). As such, immunoreactivity was still confined to the cytoplasm of the myoepithelial cells and was not detected in luminal epithelial cells. Similar results were also obtained with tissues isolated from the Balb/c strain of mice (data not shown), the strain from which SCp2 cells had been derived [11]. In addition to pregnancy, mammary glands also proliferate extensively at the onset of puberty and, in these glands, the site of intense mitotic activity resides in specialized structures called the terminal end buds [12]. In terminal end buds of pubertal females, immunoreactivity was not detected either in the body cells or the cap cells (Fig. 4D) present at the tips.

Bottom Line: In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected.In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells.Id-1 is not expressed in the luminal epithelial cells of mammary glands.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Life Sciences, Lawrence Berkeley National Laboratory, University of California Berkeley, USA.

ABSTRACT

Background: The family of inhibitor of differentiation/DNA binding (Id) proteins is known to regulate development in several tissues. One member of this gene family, Id-1, has been implicated in mammary development and carcinogenesis. Mammary glands contain various cell types, among which the luminal epithelial cells are primarily targeted for proliferation, differentiation and carcinogenesis. Therefore, to assess the precise significance of Id-1 in mammary biology and carcinogenesis, we examined its cellular localization in vivo using immunohistochemistry.

Methods: Extracts of whole mammary glands from wild type and Id-1 mutant mice, and tissue sections from paraffin-embedded mouse mammary glands from various developmental stages and normal human breast were subjected to immunoblot and immunohistochemical analyses, respectively. In both these procedures, an anti-Id-1 rabbit polyclonal antibody was used for detection of Id-1.

Results: In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected. In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells.

Conclusion: Id-1 is not expressed in the luminal epithelial cells of mammary glands.

Show MeSH
Related in: MedlinePlus