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The spatiotemporal expression pattern of the bone morphogenetic protein family in rat ovary cell types during the estrous cycle.

Erickson GF, Shimasaki S - Reprod. Biol. Endocrinol. (2003)

Bottom Line: Here we have performed a detailed in situ hybridization analysis of the spatial and temporal expression patterns of the BMP ligands (BMP-2, -3, -3b, -4, -6, -7, -15), receptors (BMPR-IA, -IB, -II), and BMP antagonist, follistatin, in rat ovaries over the normal estrous cycle.We have found that: i) all of the mRNAs are expressed in a cell-specific manner in the major classes of ovary cell types (oocyte, granulosa, theca interstitial, theca externa, corpora lutea, secondary interstitial, vascular and ovary surface epithelium); and ii) most undergo dynamic changes during follicular and corpora luteal morphogenesis and histogenesis.These results lead us to hypothesize previously unanticipated roles for the BMP family in determining fundamental developmental events that ensure the proper timing and developmental events required for the generation of the estrous cycle.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0674, USA. gerickson@ucsd.edu

ABSTRACT
In the mammalian ovary, great interest in the expression and function of the bone morphogenetic protein (BMP) family has been recently generated from evidence of their critical role in determining folliculogenesis and female fertility. Despite extensive work, there is a need to understand the cellular sites of expression of these important regulatory molecules, and how their gene expression changes within the basic ovary cell types through the cycle. Here we have performed a detailed in situ hybridization analysis of the spatial and temporal expression patterns of the BMP ligands (BMP-2, -3, -3b, -4, -6, -7, -15), receptors (BMPR-IA, -IB, -II), and BMP antagonist, follistatin, in rat ovaries over the normal estrous cycle. We have found that: i) all of the mRNAs are expressed in a cell-specific manner in the major classes of ovary cell types (oocyte, granulosa, theca interstitial, theca externa, corpora lutea, secondary interstitial, vascular and ovary surface epithelium); and ii) most undergo dynamic changes during follicular and corpora luteal morphogenesis and histogenesis. The general principle to emerge from these studies is that the developmental programs of folliculogenesis (recruitment, selection, atresia), ovulation, and luteogenesis (luteinization, luteolysis) are accompanied by rather dramatic spatial and temporal changes in the expression patterns of these BMP genes. These results lead us to hypothesize previously unanticipated roles for the BMP family in determining fundamental developmental events that ensure the proper timing and developmental events required for the generation of the estrous cycle.

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In situ hybridization of BMPR-IA mRNA in ovaries of adult cycling rats. Brighfield (A, C, D, E, G, I) and Darkfield (B, F, H, J). A, B: Sections from DII 1100 h ovaries (4X). Positive oocyte; C: Positive oocytes (O) and GC in primordial and primary follicles at P 1000 h (40X); D: Positive secondary follicle with three layers of GC at DII 1100 h (20X); E, F: Positive oocyte of an early tertiary follicle at P 1000 h. Note expression in TI (arrowheads) (10X); G, H: Preovulatory follicle at E 1000 h showing positive oocyte (O); Note weak signal in GC and TI (4X); I, J: Atretic follicle at E 1000 h showing positive O and GC (10X).
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Figure 7: In situ hybridization of BMPR-IA mRNA in ovaries of adult cycling rats. Brighfield (A, C, D, E, G, I) and Darkfield (B, F, H, J). A, B: Sections from DII 1100 h ovaries (4X). Positive oocyte; C: Positive oocytes (O) and GC in primordial and primary follicles at P 1000 h (40X); D: Positive secondary follicle with three layers of GC at DII 1100 h (20X); E, F: Positive oocyte of an early tertiary follicle at P 1000 h. Note expression in TI (arrowheads) (10X); G, H: Preovulatory follicle at E 1000 h showing positive oocyte (O); Note weak signal in GC and TI (4X); I, J: Atretic follicle at E 1000 h showing positive O and GC (10X).

Mentions: The BMPR-IA mRNA was ubiquitously expressed in the adult rat ovary, indicating a broad function in a variety of ovary cell types during the cycle (Table 1, Fig. 7A,7B). The highest expression was in the oocytes, GC, theca, and CL, with the greatest concentrations appearing in developing oocytes (Table 1). Constitutively low to moderate expression was seen in the vascular system, SIC, SE, SC, and TE (Table 1).


The spatiotemporal expression pattern of the bone morphogenetic protein family in rat ovary cell types during the estrous cycle.

Erickson GF, Shimasaki S - Reprod. Biol. Endocrinol. (2003)

In situ hybridization of BMPR-IA mRNA in ovaries of adult cycling rats. Brighfield (A, C, D, E, G, I) and Darkfield (B, F, H, J). A, B: Sections from DII 1100 h ovaries (4X). Positive oocyte; C: Positive oocytes (O) and GC in primordial and primary follicles at P 1000 h (40X); D: Positive secondary follicle with three layers of GC at DII 1100 h (20X); E, F: Positive oocyte of an early tertiary follicle at P 1000 h. Note expression in TI (arrowheads) (10X); G, H: Preovulatory follicle at E 1000 h showing positive oocyte (O); Note weak signal in GC and TI (4X); I, J: Atretic follicle at E 1000 h showing positive O and GC (10X).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC153494&req=5

Figure 7: In situ hybridization of BMPR-IA mRNA in ovaries of adult cycling rats. Brighfield (A, C, D, E, G, I) and Darkfield (B, F, H, J). A, B: Sections from DII 1100 h ovaries (4X). Positive oocyte; C: Positive oocytes (O) and GC in primordial and primary follicles at P 1000 h (40X); D: Positive secondary follicle with three layers of GC at DII 1100 h (20X); E, F: Positive oocyte of an early tertiary follicle at P 1000 h. Note expression in TI (arrowheads) (10X); G, H: Preovulatory follicle at E 1000 h showing positive oocyte (O); Note weak signal in GC and TI (4X); I, J: Atretic follicle at E 1000 h showing positive O and GC (10X).
Mentions: The BMPR-IA mRNA was ubiquitously expressed in the adult rat ovary, indicating a broad function in a variety of ovary cell types during the cycle (Table 1, Fig. 7A,7B). The highest expression was in the oocytes, GC, theca, and CL, with the greatest concentrations appearing in developing oocytes (Table 1). Constitutively low to moderate expression was seen in the vascular system, SIC, SE, SC, and TE (Table 1).

Bottom Line: Here we have performed a detailed in situ hybridization analysis of the spatial and temporal expression patterns of the BMP ligands (BMP-2, -3, -3b, -4, -6, -7, -15), receptors (BMPR-IA, -IB, -II), and BMP antagonist, follistatin, in rat ovaries over the normal estrous cycle.We have found that: i) all of the mRNAs are expressed in a cell-specific manner in the major classes of ovary cell types (oocyte, granulosa, theca interstitial, theca externa, corpora lutea, secondary interstitial, vascular and ovary surface epithelium); and ii) most undergo dynamic changes during follicular and corpora luteal morphogenesis and histogenesis.These results lead us to hypothesize previously unanticipated roles for the BMP family in determining fundamental developmental events that ensure the proper timing and developmental events required for the generation of the estrous cycle.

View Article: PubMed Central - HTML - PubMed

Affiliation: University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0674, USA. gerickson@ucsd.edu

ABSTRACT
In the mammalian ovary, great interest in the expression and function of the bone morphogenetic protein (BMP) family has been recently generated from evidence of their critical role in determining folliculogenesis and female fertility. Despite extensive work, there is a need to understand the cellular sites of expression of these important regulatory molecules, and how their gene expression changes within the basic ovary cell types through the cycle. Here we have performed a detailed in situ hybridization analysis of the spatial and temporal expression patterns of the BMP ligands (BMP-2, -3, -3b, -4, -6, -7, -15), receptors (BMPR-IA, -IB, -II), and BMP antagonist, follistatin, in rat ovaries over the normal estrous cycle. We have found that: i) all of the mRNAs are expressed in a cell-specific manner in the major classes of ovary cell types (oocyte, granulosa, theca interstitial, theca externa, corpora lutea, secondary interstitial, vascular and ovary surface epithelium); and ii) most undergo dynamic changes during follicular and corpora luteal morphogenesis and histogenesis. The general principle to emerge from these studies is that the developmental programs of folliculogenesis (recruitment, selection, atresia), ovulation, and luteogenesis (luteinization, luteolysis) are accompanied by rather dramatic spatial and temporal changes in the expression patterns of these BMP genes. These results lead us to hypothesize previously unanticipated roles for the BMP family in determining fundamental developmental events that ensure the proper timing and developmental events required for the generation of the estrous cycle.

Show MeSH
Related in: MedlinePlus