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Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Chen VT, Peng C, Leung PC - Reprod. Biol. Endocrinol. (2003)

Bottom Line: ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml.Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment.In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada. victorchen@shaw.ca

ABSTRACT
Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.

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Effects of inhibin-A on basal and activin-A-induced ActRI mRNA levels in IEVT cells. Cells were treated with inhibin-A (10 ng/ml), activin-A (10 ng/ml) or the combination of both for 6 h. ActRI mRNA levels were measured by cPCR and then normalized for β-actin mRNA levels. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).
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Figure 6: Effects of inhibin-A on basal and activin-A-induced ActRI mRNA levels in IEVT cells. Cells were treated with inhibin-A (10 ng/ml), activin-A (10 ng/ml) or the combination of both for 6 h. ActRI mRNA levels were measured by cPCR and then normalized for β-actin mRNA levels. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).

Mentions: Inhibin-A has opposing effects as activin-A in many biological systems. In this study, we also examined the effect of inhibin-A on ActRI mRNA levels. Inhibin-A (10 ng/ml) induced a 60% reduction in ActRI mRNA levels (p < 0.01) within six hours of treatment. When inhibin-A was applied together with activin-A (10 ng/ml), significant reductions in activin-A-stimulated ActRI mRNA levels were observed (Fig. 6).


Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Chen VT, Peng C, Leung PC - Reprod. Biol. Endocrinol. (2003)

Effects of inhibin-A on basal and activin-A-induced ActRI mRNA levels in IEVT cells. Cells were treated with inhibin-A (10 ng/ml), activin-A (10 ng/ml) or the combination of both for 6 h. ActRI mRNA levels were measured by cPCR and then normalized for β-actin mRNA levels. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC153493&req=5

Figure 6: Effects of inhibin-A on basal and activin-A-induced ActRI mRNA levels in IEVT cells. Cells were treated with inhibin-A (10 ng/ml), activin-A (10 ng/ml) or the combination of both for 6 h. ActRI mRNA levels were measured by cPCR and then normalized for β-actin mRNA levels. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).
Mentions: Inhibin-A has opposing effects as activin-A in many biological systems. In this study, we also examined the effect of inhibin-A on ActRI mRNA levels. Inhibin-A (10 ng/ml) induced a 60% reduction in ActRI mRNA levels (p < 0.01) within six hours of treatment. When inhibin-A was applied together with activin-A (10 ng/ml), significant reductions in activin-A-stimulated ActRI mRNA levels were observed (Fig. 6).

Bottom Line: ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml.Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment.In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada. victorchen@shaw.ca

ABSTRACT
Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.

Show MeSH
Related in: MedlinePlus