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Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Chen VT, Peng C, Leung PC - Reprod. Biol. Endocrinol. (2003)

Bottom Line: ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml.Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment.In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada. victorchen@shaw.ca

ABSTRACT
Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.

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Interaction between activin-A and follistatin-288 on ActRI mRNA expression. Cells were incubated for 6 hours in the presence or absence of activin-A (10 ng/ml) alone, or activin-A(10 ng/ml) incombination with increasing concentrations of follistatin (10–100 ng/ml). cPCR was performed to obtain ActRI mRNA levels which were then normalized for β-actin mRNA levels measured by PCR. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).
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Figure 5: Interaction between activin-A and follistatin-288 on ActRI mRNA expression. Cells were incubated for 6 hours in the presence or absence of activin-A (10 ng/ml) alone, or activin-A(10 ng/ml) incombination with increasing concentrations of follistatin (10–100 ng/ml). cPCR was performed to obtain ActRI mRNA levels which were then normalized for β-actin mRNA levels measured by PCR. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).

Mentions: The activin-binding protein, follistatin, has been shown to neutralize the effects of activin in several biological systems [24-27]. To determine the specificity of activin-A action, we examined the effects of increasing concentrations of recombinant human follistatin-288 on activin-A-induced ActRI mRNA levels. Treatment with activin-A alone resulted in a significant increase in ActRI mRNA levels (P < 0.05); however, when activin-A and follistatin were added to the culture together, the activin-A-stimulated ActRI mRNA levels were decreased. At the doses of 50 and 100 ng/ml, follistatin-288 completely blocked the effects of activin-A on the ActRI mRNA levels (Fig. 5).


Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Chen VT, Peng C, Leung PC - Reprod. Biol. Endocrinol. (2003)

Interaction between activin-A and follistatin-288 on ActRI mRNA expression. Cells were incubated for 6 hours in the presence or absence of activin-A (10 ng/ml) alone, or activin-A(10 ng/ml) incombination with increasing concentrations of follistatin (10–100 ng/ml). cPCR was performed to obtain ActRI mRNA levels which were then normalized for β-actin mRNA levels measured by PCR. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC153493&req=5

Figure 5: Interaction between activin-A and follistatin-288 on ActRI mRNA expression. Cells were incubated for 6 hours in the presence or absence of activin-A (10 ng/ml) alone, or activin-A(10 ng/ml) incombination with increasing concentrations of follistatin (10–100 ng/ml). cPCR was performed to obtain ActRI mRNA levels which were then normalized for β-actin mRNA levels measured by PCR. Data present mean ± SEM of three experiments. Different letters indicate statistically significant differences (p < 0.05).
Mentions: The activin-binding protein, follistatin, has been shown to neutralize the effects of activin in several biological systems [24-27]. To determine the specificity of activin-A action, we examined the effects of increasing concentrations of recombinant human follistatin-288 on activin-A-induced ActRI mRNA levels. Treatment with activin-A alone resulted in a significant increase in ActRI mRNA levels (P < 0.05); however, when activin-A and follistatin were added to the culture together, the activin-A-stimulated ActRI mRNA levels were decreased. At the doses of 50 and 100 ng/ml, follistatin-288 completely blocked the effects of activin-A on the ActRI mRNA levels (Fig. 5).

Bottom Line: ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml.Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment.In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada. victorchen@shaw.ca

ABSTRACT
Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.

Show MeSH
Related in: MedlinePlus