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Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Chen VT, Peng C, Leung PC - Reprod. Biol. Endocrinol. (2003)

Bottom Line: ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml.Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment.In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada. victorchen@shaw.ca

ABSTRACT
Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.

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Time course effects of activin-A on ActRI mRNA levels. IEVT cells were incubated with or without activin-A (10 ng/ml) for 1, 3, 6, 12, 24 hours. cPCR was performed to measure ActRI mRNA levels and data were also normalized for β-actin value. *,(p < 0.05) versus control. **,(p < 0.01) vs control. Data present mean ± SEM of three experiments.
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Figure 4: Time course effects of activin-A on ActRI mRNA levels. IEVT cells were incubated with or without activin-A (10 ng/ml) for 1, 3, 6, 12, 24 hours. cPCR was performed to measure ActRI mRNA levels and data were also normalized for β-actin value. *,(p < 0.05) versus control. **,(p < 0.01) vs control. Data present mean ± SEM of three experiments.

Mentions: The ActRI mRNA level, measured at 12 hours after treatment, was increased in a dose-dependent manner by activin-A (Fig. 3). A significant stimulation (P < 0.05) of ActRI mRNA levels was observed at the dose of 3, 10 and 30 ng/ml. When IEVT cells were treated with activin-A (10 ng/ml) for different period of time, a significant increase in ActRI mRNA level was found at 3 hours (P < 0.05) and the maximal response observed at 6 hours (P < 0.01) after treatment. By 24 h after treatment, ActRI mRNA levels in activin-A treated cells were not significantly different from those in the control cells (Fig. 4).


Activin-A up-regulates type I activin receptor mRNA levels in human immortalized extravillous trophoblast cells.

Chen VT, Peng C, Leung PC - Reprod. Biol. Endocrinol. (2003)

Time course effects of activin-A on ActRI mRNA levels. IEVT cells were incubated with or without activin-A (10 ng/ml) for 1, 3, 6, 12, 24 hours. cPCR was performed to measure ActRI mRNA levels and data were also normalized for β-actin value. *,(p < 0.05) versus control. **,(p < 0.01) vs control. Data present mean ± SEM of three experiments.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC153493&req=5

Figure 4: Time course effects of activin-A on ActRI mRNA levels. IEVT cells were incubated with or without activin-A (10 ng/ml) for 1, 3, 6, 12, 24 hours. cPCR was performed to measure ActRI mRNA levels and data were also normalized for β-actin value. *,(p < 0.05) versus control. **,(p < 0.01) vs control. Data present mean ± SEM of three experiments.
Mentions: The ActRI mRNA level, measured at 12 hours after treatment, was increased in a dose-dependent manner by activin-A (Fig. 3). A significant stimulation (P < 0.05) of ActRI mRNA levels was observed at the dose of 3, 10 and 30 ng/ml. When IEVT cells were treated with activin-A (10 ng/ml) for different period of time, a significant increase in ActRI mRNA level was found at 3 hours (P < 0.05) and the maximal response observed at 6 hours (P < 0.01) after treatment. By 24 h after treatment, ActRI mRNA levels in activin-A treated cells were not significantly different from those in the control cells (Fig. 4).

Bottom Line: ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml.Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment.In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada. victorchen@shaw.ca

ABSTRACT
Activin is known to play an important regulatory role in reproduction, including pregnancy. To further examine the role and signaling mechanism of activin in regulating placental function, the steady-state level of activin type I receptor (ActRI) mRNA in immortalized extravillous trophoblasts (IEVT) cells was measured using competitive PCR (cPCR). An internal standard of ActRI cDNA for cPCR was constructed for the quantification of ActRI mRNA levels in IEVT cells. ActRI mRNA levels were increased in a dose-dependent manner by activin-A with the maximal effect observed at the dose of 10 ng/ml. Time course studies revealed that activin-A had maximal effects on ActRI mRNA levels at 6 hours after treatment. The effects of activin-A on ActRI mRNA levels was blocked by follistatin, an activin binding protein, in a dose-dependent manner. In addition, inhibin-A inhibited basal, as well as activin-A-induced ActRI mRNA levels. These findings provide evidence, for the first time, that activin-A modulates ActRI mRNA levels in human trophoblast cells.

Show MeSH
Related in: MedlinePlus