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Plasma PGE-2 levels and altered cytokine profiles in adherent peripheral blood mononuclear cells in non-small cell lung cancer (NSCLC).

Hidalgo GE, Zhong L, Doherty DE, Hirschowitz EA - Mol. Cancer (2002)

Bottom Line: PGE-2 is constitutively produced by many non-small cell lung cancers (NSCLC) and its immunosuppressive effects have been linked to altered immune responses in lung cancer.Data show plasma PGE-2 levels in 38 NSCLC patients are elevated compared to normal controls.Further investigation of each as a nonspecific marker for NSCLC tumor is warranted.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary and Critical Care Medicine, Lexington Veteran's Administration Medical Center, University of Kentucky, Chandler Medical Center, 800 Rose Street, Lexington, Kentucky 40536, USA. ghida2@pop.uky.edu

ABSTRACT

Introduction: PGE-2 is constitutively produced by many non-small cell lung cancers (NSCLC) and its immunosuppressive effects have been linked to altered immune responses in lung cancer. We asked whether elevated levels of plasma PGE-2 correlated with monocyte IL10 production in the NSCLC environment. Looking for correlation in NSCLC patient blood we assayed plasma from NSCLC patients for PGE2 and IL10; we further evaluated production of IL10 by adherent mononuclear cells from a subset of these patients looking for an altered cytokine profile.

Results: Our initial in vitro experiments show that monocyte IL10 induction correlates with tumor cell PGE-2 production, confirming similar reports in the literature. Data show plasma PGE-2 levels in 38 NSCLC patients are elevated compared to normal controls. Plasma IL10 levels were not significantly elevated; however, adherent monocytes derived from NSCLC patient blood did produce significantly more IL10 in 24 hr primary culture than those from normal controls (p < 0.01). The association of elevated plasma PGE-2 and monocyte derived IL-10 was not significant.

Conclusions: Elevated plasma PGE-2 and monocyte IL10 production are associated with NSCLC. The biological significance to elevated PGE-2 levels in NSCLC are unclear. Further investigation of each as a nonspecific marker for NSCLC tumor is warranted.

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Production of PGE-2 and induction of IL10 by NSCLC tumor cell lines. Supernatants from 6 established cell lines and control cell line 293 (5 × 105/ml) were assayed for PGE-2 over 24 hr by ELISA. Data are presented as mean of triplicate samples and are expressed as pg/ml/5 × 105 cells. Supernatants were transferred to adherent mononuclear cells from healthy volunteers (106/ml) and IL10 was assayed in supernatants at 24 hr. Data are expressed as mean +/- SEM.
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Figure 1: Production of PGE-2 and induction of IL10 by NSCLC tumor cell lines. Supernatants from 6 established cell lines and control cell line 293 (5 × 105/ml) were assayed for PGE-2 over 24 hr by ELISA. Data are presented as mean of triplicate samples and are expressed as pg/ml/5 × 105 cells. Supernatants were transferred to adherent mononuclear cells from healthy volunteers (106/ml) and IL10 was assayed in supernatants at 24 hr. Data are expressed as mean +/- SEM.

Mentions: To confirm observations from the literature, supernatants from 6 different NSCLC cell lines producing variable amounts of PGE-2 (ranging from 100 pg/ml/5 × 105 cells to >50,000 pg/ml/5 × 105 cells) were compared directly for their ability to induce IL10 in adherent mononuclear cells. Each cell line induced IL10 in adherent mononuclear cells that was greater than control. This relative increase in IL10 correlated with PGE-2 produced by each cell line (p < 0.05).(Figure 1).


Plasma PGE-2 levels and altered cytokine profiles in adherent peripheral blood mononuclear cells in non-small cell lung cancer (NSCLC).

Hidalgo GE, Zhong L, Doherty DE, Hirschowitz EA - Mol. Cancer (2002)

Production of PGE-2 and induction of IL10 by NSCLC tumor cell lines. Supernatants from 6 established cell lines and control cell line 293 (5 × 105/ml) were assayed for PGE-2 over 24 hr by ELISA. Data are presented as mean of triplicate samples and are expressed as pg/ml/5 × 105 cells. Supernatants were transferred to adherent mononuclear cells from healthy volunteers (106/ml) and IL10 was assayed in supernatants at 24 hr. Data are expressed as mean +/- SEM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC149408&req=5

Figure 1: Production of PGE-2 and induction of IL10 by NSCLC tumor cell lines. Supernatants from 6 established cell lines and control cell line 293 (5 × 105/ml) were assayed for PGE-2 over 24 hr by ELISA. Data are presented as mean of triplicate samples and are expressed as pg/ml/5 × 105 cells. Supernatants were transferred to adherent mononuclear cells from healthy volunteers (106/ml) and IL10 was assayed in supernatants at 24 hr. Data are expressed as mean +/- SEM.
Mentions: To confirm observations from the literature, supernatants from 6 different NSCLC cell lines producing variable amounts of PGE-2 (ranging from 100 pg/ml/5 × 105 cells to >50,000 pg/ml/5 × 105 cells) were compared directly for their ability to induce IL10 in adherent mononuclear cells. Each cell line induced IL10 in adherent mononuclear cells that was greater than control. This relative increase in IL10 correlated with PGE-2 produced by each cell line (p < 0.05).(Figure 1).

Bottom Line: PGE-2 is constitutively produced by many non-small cell lung cancers (NSCLC) and its immunosuppressive effects have been linked to altered immune responses in lung cancer.Data show plasma PGE-2 levels in 38 NSCLC patients are elevated compared to normal controls.Further investigation of each as a nonspecific marker for NSCLC tumor is warranted.

View Article: PubMed Central - HTML - PubMed

Affiliation: Division of Pulmonary and Critical Care Medicine, Lexington Veteran's Administration Medical Center, University of Kentucky, Chandler Medical Center, 800 Rose Street, Lexington, Kentucky 40536, USA. ghida2@pop.uky.edu

ABSTRACT

Introduction: PGE-2 is constitutively produced by many non-small cell lung cancers (NSCLC) and its immunosuppressive effects have been linked to altered immune responses in lung cancer. We asked whether elevated levels of plasma PGE-2 correlated with monocyte IL10 production in the NSCLC environment. Looking for correlation in NSCLC patient blood we assayed plasma from NSCLC patients for PGE2 and IL10; we further evaluated production of IL10 by adherent mononuclear cells from a subset of these patients looking for an altered cytokine profile.

Results: Our initial in vitro experiments show that monocyte IL10 induction correlates with tumor cell PGE-2 production, confirming similar reports in the literature. Data show plasma PGE-2 levels in 38 NSCLC patients are elevated compared to normal controls. Plasma IL10 levels were not significantly elevated; however, adherent monocytes derived from NSCLC patient blood did produce significantly more IL10 in 24 hr primary culture than those from normal controls (p < 0.01). The association of elevated plasma PGE-2 and monocyte derived IL-10 was not significant.

Conclusions: Elevated plasma PGE-2 and monocyte IL10 production are associated with NSCLC. The biological significance to elevated PGE-2 levels in NSCLC are unclear. Further investigation of each as a nonspecific marker for NSCLC tumor is warranted.

Show MeSH
Related in: MedlinePlus