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Mapping of neurokinin-like immunoreactivity in the human brainstem.

Coveñas R, Martin F, Belda M, Smith V, Salinas P, Rivada E, Diaz-Cabiale Z, Narvaez JA, Marcos P, Tramu G, Gonzalez-Baron S - BMC Neurosci (2003)

Bottom Line: Moreover, immunoreactive cell bodies were found in the inferior colliculus, the raphe obscurus, the nucleus prepositus hypoglossi, and in the midline of the anterior medulla oblongata.In addition to the nuclei mentioned above, the highest densities of such immunoreactive fibers were located in the spinal trigeminal nucleus, the lateral reticular nucleus, the nucleus of the solitary tract, the superior colliculus, the substantia nigra, the nucleus ambiguus, the gracile nucleus, the cuneate nucleus, the motor hypoglossal nucleus, the medial and superior vestibular nuclei, the nucleus prepositus hypoglossi and the interpeduncular nucleus.The widespread distribution of immunoreactive structures containing neurokinin in the human brainstem indicates that neurokinin might be involved in several physiological mechanisms, acting as a neurotransmitter and/or neuromodulator.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neurosciences of Castilla y León (INCYL), School of Medicine, Laboratory of Neuroanatomy of the Peptidergic Systems, Salamanca, Spain. covenas@gugu.usal.es

ABSTRACT

Background: Using an indirect immunoperoxidase technique, we have studied the distribution of immunoreactive fibers and cell bodies containing neurokinin in the adult human brainstem with no prior history of neurological or psychiatric disease.

Results: Clusters of immunoreactive cell bodies and high densities of neurokinin-immunoreactive fibers were located in the periaqueductal gray, the dorsal motor nucleus of the vagus and in the reticular formation of the medulla, pons and mesencephalon. Moreover, immunoreactive cell bodies were found in the inferior colliculus, the raphe obscurus, the nucleus prepositus hypoglossi, and in the midline of the anterior medulla oblongata. In general, immunoreactive fibers containing neurokinin were observed throughout the whole brainstem. In addition to the nuclei mentioned above, the highest densities of such immunoreactive fibers were located in the spinal trigeminal nucleus, the lateral reticular nucleus, the nucleus of the solitary tract, the superior colliculus, the substantia nigra, the nucleus ambiguus, the gracile nucleus, the cuneate nucleus, the motor hypoglossal nucleus, the medial and superior vestibular nuclei, the nucleus prepositus hypoglossi and the interpeduncular nucleus.

Conclusion: The widespread distribution of immunoreactive structures containing neurokinin in the human brainstem indicates that neurokinin might be involved in several physiological mechanisms, acting as a neurotransmitter and/or neuromodulator.

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A. Cell bodies (arrows) and fibers (arrowheads) in the dorsal motor nucleus of the vagus (DMNu). Note NK-ir fibers (arrowheads) in both nucleus of the solitary tract (SolNu) and in the solitary tract (SolTr). In addition, NK-ir fibers can be observed around the nucleus of the solitary tract. D: dorsal; M: medial. Scale bar: 100 μm B. High-power image of the area delimited in Figure 5A. D: dorsal; M: medial. Scale bar: 100 μm
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Figure 5: A. Cell bodies (arrows) and fibers (arrowheads) in the dorsal motor nucleus of the vagus (DMNu). Note NK-ir fibers (arrowheads) in both nucleus of the solitary tract (SolNu) and in the solitary tract (SolTr). In addition, NK-ir fibers can be observed around the nucleus of the solitary tract. D: dorsal; M: medial. Scale bar: 100 μm B. High-power image of the area delimited in Figure 5A. D: dorsal; M: medial. Scale bar: 100 μm

Mentions: A low density of immunoreactive cell bodies containing NK was observed caudally in the medullary central gray (Figs. 1A; 4A), whereas more rostrally (Fig. 1B) such clusters of NK-ir perikarya showed a moderate density. At the latter level (Fig. 1B), a moderate density was also observed in the dorsal motor nucleus of the vagus and a low density below the nucleus of the solitary tract. More rostrally (Fig. 1C) three populations of immunoreactive cell bodies were observed: the first located along the midline (high density); the second in the dorsal motor nucleus of the vagus (high density)(Fig. 5A,5B); and the third in the reticular formation (nucleus reticularis gigantocellularis included)(moderate density), above the dorsal accessory olivary nucleus and the central tegmental tract (Fig. 6A,6B). In addition, a moderate density of immunoreactive cell bodies containing NK was observed in the raphe obscurus (not shown in Figures).


Mapping of neurokinin-like immunoreactivity in the human brainstem.

Coveñas R, Martin F, Belda M, Smith V, Salinas P, Rivada E, Diaz-Cabiale Z, Narvaez JA, Marcos P, Tramu G, Gonzalez-Baron S - BMC Neurosci (2003)

A. Cell bodies (arrows) and fibers (arrowheads) in the dorsal motor nucleus of the vagus (DMNu). Note NK-ir fibers (arrowheads) in both nucleus of the solitary tract (SolNu) and in the solitary tract (SolTr). In addition, NK-ir fibers can be observed around the nucleus of the solitary tract. D: dorsal; M: medial. Scale bar: 100 μm B. High-power image of the area delimited in Figure 5A. D: dorsal; M: medial. Scale bar: 100 μm
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC149367&req=5

Figure 5: A. Cell bodies (arrows) and fibers (arrowheads) in the dorsal motor nucleus of the vagus (DMNu). Note NK-ir fibers (arrowheads) in both nucleus of the solitary tract (SolNu) and in the solitary tract (SolTr). In addition, NK-ir fibers can be observed around the nucleus of the solitary tract. D: dorsal; M: medial. Scale bar: 100 μm B. High-power image of the area delimited in Figure 5A. D: dorsal; M: medial. Scale bar: 100 μm
Mentions: A low density of immunoreactive cell bodies containing NK was observed caudally in the medullary central gray (Figs. 1A; 4A), whereas more rostrally (Fig. 1B) such clusters of NK-ir perikarya showed a moderate density. At the latter level (Fig. 1B), a moderate density was also observed in the dorsal motor nucleus of the vagus and a low density below the nucleus of the solitary tract. More rostrally (Fig. 1C) three populations of immunoreactive cell bodies were observed: the first located along the midline (high density); the second in the dorsal motor nucleus of the vagus (high density)(Fig. 5A,5B); and the third in the reticular formation (nucleus reticularis gigantocellularis included)(moderate density), above the dorsal accessory olivary nucleus and the central tegmental tract (Fig. 6A,6B). In addition, a moderate density of immunoreactive cell bodies containing NK was observed in the raphe obscurus (not shown in Figures).

Bottom Line: Moreover, immunoreactive cell bodies were found in the inferior colliculus, the raphe obscurus, the nucleus prepositus hypoglossi, and in the midline of the anterior medulla oblongata.In addition to the nuclei mentioned above, the highest densities of such immunoreactive fibers were located in the spinal trigeminal nucleus, the lateral reticular nucleus, the nucleus of the solitary tract, the superior colliculus, the substantia nigra, the nucleus ambiguus, the gracile nucleus, the cuneate nucleus, the motor hypoglossal nucleus, the medial and superior vestibular nuclei, the nucleus prepositus hypoglossi and the interpeduncular nucleus.The widespread distribution of immunoreactive structures containing neurokinin in the human brainstem indicates that neurokinin might be involved in several physiological mechanisms, acting as a neurotransmitter and/or neuromodulator.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Neurosciences of Castilla y León (INCYL), School of Medicine, Laboratory of Neuroanatomy of the Peptidergic Systems, Salamanca, Spain. covenas@gugu.usal.es

ABSTRACT

Background: Using an indirect immunoperoxidase technique, we have studied the distribution of immunoreactive fibers and cell bodies containing neurokinin in the adult human brainstem with no prior history of neurological or psychiatric disease.

Results: Clusters of immunoreactive cell bodies and high densities of neurokinin-immunoreactive fibers were located in the periaqueductal gray, the dorsal motor nucleus of the vagus and in the reticular formation of the medulla, pons and mesencephalon. Moreover, immunoreactive cell bodies were found in the inferior colliculus, the raphe obscurus, the nucleus prepositus hypoglossi, and in the midline of the anterior medulla oblongata. In general, immunoreactive fibers containing neurokinin were observed throughout the whole brainstem. In addition to the nuclei mentioned above, the highest densities of such immunoreactive fibers were located in the spinal trigeminal nucleus, the lateral reticular nucleus, the nucleus of the solitary tract, the superior colliculus, the substantia nigra, the nucleus ambiguus, the gracile nucleus, the cuneate nucleus, the motor hypoglossal nucleus, the medial and superior vestibular nuclei, the nucleus prepositus hypoglossi and the interpeduncular nucleus.

Conclusion: The widespread distribution of immunoreactive structures containing neurokinin in the human brainstem indicates that neurokinin might be involved in several physiological mechanisms, acting as a neurotransmitter and/or neuromodulator.

Show MeSH
Related in: MedlinePlus