Limits...
Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review.

Braet F, Wisse E - Comp Hepatol (2002)

Bottom Line: This review provides a detailed overview of the current state of knowledge about the ultrastructure and dynamics of liver sinusoidal endothelial fenestrae.Various aspects of liver sinusoidal endothelial fenestrae regarding their structure, origin, species specificity, dynamics and formation will be explored.In addition, the role of liver sinusoidal endothelial fenestrae in relation to lipoprotein metabolism, fibrosis and cancer will be approached.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory for Cell Biology and Histology, Free University of Brussels (VUB), Laarbeeklaan 103, 1090 Brussels-Jette, Belgium. filipbra@cyto.vub.ac.be

ABSTRACT
This review provides a detailed overview of the current state of knowledge about the ultrastructure and dynamics of liver sinusoidal endothelial fenestrae. Various aspects of liver sinusoidal endothelial fenestrae regarding their structure, origin, species specificity, dynamics and formation will be explored. In addition, the role of liver sinusoidal endothelial fenestrae in relation to lipoprotein metabolism, fibrosis and cancer will be approached.

No MeSH data available.


Related in: MedlinePlus

High-power scanning electron micrographs of a nonextracted (left); and of a formaldehyde prefixed, cytoskeleton buffer extrated rat liver sinusoidal endothelial cell (middle). Notice the grouped fenestrae on the cell surface (left); and a remarkable series of rings of fenestrae-associated cytoskeleton (middle). Layering a colored scanning electron micrograph on top of the cell surface of a nonextracted rat liver sinusoidal endothelial cell clearly illustrates the relation between both structures (right). Scale bar, 200 nm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC131011&req=5

Figure 4: High-power scanning electron micrographs of a nonextracted (left); and of a formaldehyde prefixed, cytoskeleton buffer extrated rat liver sinusoidal endothelial cell (middle). Notice the grouped fenestrae on the cell surface (left); and a remarkable series of rings of fenestrae-associated cytoskeleton (middle). Layering a colored scanning electron micrograph on top of the cell surface of a nonextracted rat liver sinusoidal endothelial cell clearly illustrates the relation between both structures (right). Scale bar, 200 nm.

Mentions: The results of our whole mount electron microscopic studies on LSEC have added the following new insights in the structure and function of the cytoskeleton in fenestrated areas [42,53]: (I) Sieve plates and fenestrae are delineated by cytoskeleton elements; (II) fenestrae are delineated by a filamentous, fenestrae-associated cytoskeleton ring (FACR) (Fig. 4) with a mean filament thickness of 16 nm, (III) sieve plates are surrounded by a ring-like orientation of microtubuli, which form a network together with additional branching cytoskeletal elements; (IV) because of the fact that the fenestrae-associated cytoskeleton ring opens and closes like fenestrae in response to different treatments such as ethanol or serotonin, it is assumed that this ring regulates the size changes of the fenestrae; and (V) therefore, the fenestrae-associated cytoskeleton probably controls the important function of endothelial filtration.


Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review.

Braet F, Wisse E - Comp Hepatol (2002)

High-power scanning electron micrographs of a nonextracted (left); and of a formaldehyde prefixed, cytoskeleton buffer extrated rat liver sinusoidal endothelial cell (middle). Notice the grouped fenestrae on the cell surface (left); and a remarkable series of rings of fenestrae-associated cytoskeleton (middle). Layering a colored scanning electron micrograph on top of the cell surface of a nonextracted rat liver sinusoidal endothelial cell clearly illustrates the relation between both structures (right). Scale bar, 200 nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC131011&req=5

Figure 4: High-power scanning electron micrographs of a nonextracted (left); and of a formaldehyde prefixed, cytoskeleton buffer extrated rat liver sinusoidal endothelial cell (middle). Notice the grouped fenestrae on the cell surface (left); and a remarkable series of rings of fenestrae-associated cytoskeleton (middle). Layering a colored scanning electron micrograph on top of the cell surface of a nonextracted rat liver sinusoidal endothelial cell clearly illustrates the relation between both structures (right). Scale bar, 200 nm.
Mentions: The results of our whole mount electron microscopic studies on LSEC have added the following new insights in the structure and function of the cytoskeleton in fenestrated areas [42,53]: (I) Sieve plates and fenestrae are delineated by cytoskeleton elements; (II) fenestrae are delineated by a filamentous, fenestrae-associated cytoskeleton ring (FACR) (Fig. 4) with a mean filament thickness of 16 nm, (III) sieve plates are surrounded by a ring-like orientation of microtubuli, which form a network together with additional branching cytoskeletal elements; (IV) because of the fact that the fenestrae-associated cytoskeleton ring opens and closes like fenestrae in response to different treatments such as ethanol or serotonin, it is assumed that this ring regulates the size changes of the fenestrae; and (V) therefore, the fenestrae-associated cytoskeleton probably controls the important function of endothelial filtration.

Bottom Line: This review provides a detailed overview of the current state of knowledge about the ultrastructure and dynamics of liver sinusoidal endothelial fenestrae.Various aspects of liver sinusoidal endothelial fenestrae regarding their structure, origin, species specificity, dynamics and formation will be explored.In addition, the role of liver sinusoidal endothelial fenestrae in relation to lipoprotein metabolism, fibrosis and cancer will be approached.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratory for Cell Biology and Histology, Free University of Brussels (VUB), Laarbeeklaan 103, 1090 Brussels-Jette, Belgium. filipbra@cyto.vub.ac.be

ABSTRACT
This review provides a detailed overview of the current state of knowledge about the ultrastructure and dynamics of liver sinusoidal endothelial fenestrae. Various aspects of liver sinusoidal endothelial fenestrae regarding their structure, origin, species specificity, dynamics and formation will be explored. In addition, the role of liver sinusoidal endothelial fenestrae in relation to lipoprotein metabolism, fibrosis and cancer will be approached.

No MeSH data available.


Related in: MedlinePlus