Limits...
From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza W - Kinetoplastid Biol Dis (2002)

Bottom Line: These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells.In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective.The possible use of available data for the development of new anti parasite drugs is also discussed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCSBloco G, 21941900, Rio de JaneiroRJ, Brasil. wsouza@biof.ufrj.br

ABSTRACT
Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells. This review analyses the process of transformation, which takes place during the life cycle of Trypanosoma cruzi in the vertebrate and invertebrate hosts. Special attention is given to the interaction of the parasite with vertebrate cells. In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective. The possible use of available data for the development of new anti parasite drugs is also discussed.

No MeSH data available.


Related in: MedlinePlus

Immunocytochemical localization of cysteine proteinase in an epimastigote form of T. cruzi. Intense labeling of the cell surface and reservosomes can be observed.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC119324&req=5

Figure 23: Immunocytochemical localization of cysteine proteinase in an epimastigote form of T. cruzi. Intense labeling of the cell surface and reservosomes can be observed.

Mentions: One characteristic feature of the reservosome in T. cruzi is the accumulation of a large amount of cruzipain, the major cysteine proteinase found in the cell (Fig. 23) [21]. Although glycosylated this protein does not possess mannose-6-phosphate residues. No mannose-6-phosphate receptors could be immunocytochemically detected in T. cruzi [83]. Therefore, another un-characterized intracellular route is used to deliver cruzipain to the endosomal system. Cruzipain has been the subject of intense investigation in the last years mainly due to its biological importance. The multicopy genes encoding this protease are part of a large array of polymorphic sequences located in different chromosomes [87,88]. The genes coding for this cathepsin L-like cysteine proteinase have been cloned and expressed and the crystal and molecular structure of the recombinant protein determined. Enzyme inhibitors, which are peptide analogs, inhibit the proliferation of epimastigote and amastigote forms and arrest the process of transformation of non-infective epimastigotes into infective trypomastigotes [23].


From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza W - Kinetoplastid Biol Dis (2002)

Immunocytochemical localization of cysteine proteinase in an epimastigote form of T. cruzi. Intense labeling of the cell surface and reservosomes can be observed.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC119324&req=5

Figure 23: Immunocytochemical localization of cysteine proteinase in an epimastigote form of T. cruzi. Intense labeling of the cell surface and reservosomes can be observed.
Mentions: One characteristic feature of the reservosome in T. cruzi is the accumulation of a large amount of cruzipain, the major cysteine proteinase found in the cell (Fig. 23) [21]. Although glycosylated this protein does not possess mannose-6-phosphate residues. No mannose-6-phosphate receptors could be immunocytochemically detected in T. cruzi [83]. Therefore, another un-characterized intracellular route is used to deliver cruzipain to the endosomal system. Cruzipain has been the subject of intense investigation in the last years mainly due to its biological importance. The multicopy genes encoding this protease are part of a large array of polymorphic sequences located in different chromosomes [87,88]. The genes coding for this cathepsin L-like cysteine proteinase have been cloned and expressed and the crystal and molecular structure of the recombinant protein determined. Enzyme inhibitors, which are peptide analogs, inhibit the proliferation of epimastigote and amastigote forms and arrest the process of transformation of non-infective epimastigotes into infective trypomastigotes [23].

Bottom Line: These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells.In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective.The possible use of available data for the development of new anti parasite drugs is also discussed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCSBloco G, 21941900, Rio de JaneiroRJ, Brasil. wsouza@biof.ufrj.br

ABSTRACT
Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells. This review analyses the process of transformation, which takes place during the life cycle of Trypanosoma cruzi in the vertebrate and invertebrate hosts. Special attention is given to the interaction of the parasite with vertebrate cells. In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective. The possible use of available data for the development of new anti parasite drugs is also discussed.

No MeSH data available.


Related in: MedlinePlus