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From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza W - Kinetoplastid Biol Dis (2002)

Bottom Line: These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells.In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective.The possible use of available data for the development of new anti parasite drugs is also discussed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCSBloco G, 21941900, Rio de JaneiroRJ, Brasil. wsouza@biof.ufrj.br

ABSTRACT
Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells. This review analyses the process of transformation, which takes place during the life cycle of Trypanosoma cruzi in the vertebrate and invertebrate hosts. Special attention is given to the interaction of the parasite with vertebrate cells. In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective. The possible use of available data for the development of new anti parasite drugs is also discussed.

No MeSH data available.


Related in: MedlinePlus

General view of an epimastigote form of T. cruzi as seen in a freeze-fracture replica. The flagellar membrane and the cytostome can be seen.
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Figure 20: General view of an epimastigote form of T. cruzi as seen in a freeze-fracture replica. The flagellar membrane and the cytostome can be seen.

Mentions: The epimastigote stage of members of the Trypanosoma genus belonging to the Schyzotrypanum subgenus, such as Trypanosoma cruzi, Trypanosoma vespertilionis and Trypanosoma dionisii, possess a flagellar pocket which differs in shape form those found in other developmental stages. In the case of epimastigote and amastigote forms of members of the subgenus Schizotrypanum there is a highly specialized structure known as the cytostome-cytopharynx complex. It appears as a funnel-shaped structure formed due to a deep invagination of the plasma membrane, which may reach the nuclear region. The opening of this complex, which is known as the cytostome, may reach a diameter of 0.3 μm and is significantly smaller in the deeper portion of the cytopharinx. The subpellicular microtubules follow the invagination of the plasma membrane. There is a specialized region of the membrane lining the parasite, which starts in the opening of the cytostome and projects towards the flagellar pocket region. Freeze-fracture studies have shown that this area is delimited from the other portions of the plasma membrane by a pallisade-like array of closely associated particles (Fig. 20) [Review in [41]].


From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza W - Kinetoplastid Biol Dis (2002)

General view of an epimastigote form of T. cruzi as seen in a freeze-fracture replica. The flagellar membrane and the cytostome can be seen.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC119324&req=5

Figure 20: General view of an epimastigote form of T. cruzi as seen in a freeze-fracture replica. The flagellar membrane and the cytostome can be seen.
Mentions: The epimastigote stage of members of the Trypanosoma genus belonging to the Schyzotrypanum subgenus, such as Trypanosoma cruzi, Trypanosoma vespertilionis and Trypanosoma dionisii, possess a flagellar pocket which differs in shape form those found in other developmental stages. In the case of epimastigote and amastigote forms of members of the subgenus Schizotrypanum there is a highly specialized structure known as the cytostome-cytopharynx complex. It appears as a funnel-shaped structure formed due to a deep invagination of the plasma membrane, which may reach the nuclear region. The opening of this complex, which is known as the cytostome, may reach a diameter of 0.3 μm and is significantly smaller in the deeper portion of the cytopharinx. The subpellicular microtubules follow the invagination of the plasma membrane. There is a specialized region of the membrane lining the parasite, which starts in the opening of the cytostome and projects towards the flagellar pocket region. Freeze-fracture studies have shown that this area is delimited from the other portions of the plasma membrane by a pallisade-like array of closely associated particles (Fig. 20) [Review in [41]].

Bottom Line: These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells.In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective.The possible use of available data for the development of new anti parasite drugs is also discussed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCSBloco G, 21941900, Rio de JaneiroRJ, Brasil. wsouza@biof.ufrj.br

ABSTRACT
Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells. This review analyses the process of transformation, which takes place during the life cycle of Trypanosoma cruzi in the vertebrate and invertebrate hosts. Special attention is given to the interaction of the parasite with vertebrate cells. In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective. The possible use of available data for the development of new anti parasite drugs is also discussed.

No MeSH data available.


Related in: MedlinePlus