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From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza W - Kinetoplastid Biol Dis (2002)

Bottom Line: These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells.In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective.The possible use of available data for the development of new anti parasite drugs is also discussed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCSBloco G, 21941900, Rio de JaneiroRJ, Brasil. wsouza@biof.ufrj.br

ABSTRACT
Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells. This review analyses the process of transformation, which takes place during the life cycle of Trypanosoma cruzi in the vertebrate and invertebrate hosts. Special attention is given to the interaction of the parasite with vertebrate cells. In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective. The possible use of available data for the development of new anti parasite drugs is also discussed.

No MeSH data available.


Related in: MedlinePlus

a and b: Two different views of the paraflagellar structure as seen in thin sections and in replicas of quick frozen, freeze-fracture and deep-etched cells.
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Figure 14: a and b: Two different views of the paraflagellar structure as seen in thin sections and in replicas of quick frozen, freeze-fracture and deep-etched cells.

Mentions: The paraflagellar rod (PFR), also known as paraflagellar structure, runs alongside the axoneme since emerging from the flagellar pocket (Fig. 14). Three distinct zones can be identified in the PFR: (a) the proximal and distal zones possess 7–10 nm thick filaments intersected at an angle of 100°, which gives the structure a lattice-like aspect. This zone is connected to doublet numbers 4 to 7 of the axoneme through filamentous bridges. (b) The intermediate zone is formed by 5 nm thick filaments which intersect at an angle of 45°. (c) The distal zone, whose size varies according to the species, is basically similar to the proximal one. Biochemical studies carried out in T. brucei have shown that the PFR is made of two major proteins of 68 and 76 kDa and several minor components. Recent studies showed that mutants induced to ablate expression of the major PFR proteins do not show a complete PFR structure. Some of these mutants show a reduced motility, altered flagellar beat pattern or even complete immobilization [Reviews in [44,45]].


From the cell biology to the development of new chemotherapeutic approaches against trypanosomatids: dreams and reality.

De Souza W - Kinetoplastid Biol Dis (2002)

a and b: Two different views of the paraflagellar structure as seen in thin sections and in replicas of quick frozen, freeze-fracture and deep-etched cells.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC119324&req=5

Figure 14: a and b: Two different views of the paraflagellar structure as seen in thin sections and in replicas of quick frozen, freeze-fracture and deep-etched cells.
Mentions: The paraflagellar rod (PFR), also known as paraflagellar structure, runs alongside the axoneme since emerging from the flagellar pocket (Fig. 14). Three distinct zones can be identified in the PFR: (a) the proximal and distal zones possess 7–10 nm thick filaments intersected at an angle of 100°, which gives the structure a lattice-like aspect. This zone is connected to doublet numbers 4 to 7 of the axoneme through filamentous bridges. (b) The intermediate zone is formed by 5 nm thick filaments which intersect at an angle of 45°. (c) The distal zone, whose size varies according to the species, is basically similar to the proximal one. Biochemical studies carried out in T. brucei have shown that the PFR is made of two major proteins of 68 and 76 kDa and several minor components. Recent studies showed that mutants induced to ablate expression of the major PFR proteins do not show a complete PFR structure. Some of these mutants show a reduced motility, altered flagellar beat pattern or even complete immobilization [Reviews in [44,45]].

Bottom Line: These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells.In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective.The possible use of available data for the development of new anti parasite drugs is also discussed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCSBloco G, 21941900, Rio de JaneiroRJ, Brasil. wsouza@biof.ufrj.br

ABSTRACT
Members of the Trypanosomatidae family comprise a large number of species that are causative agents of important diseases such as sleeping sickness, Chagas' disease and Leishmaniasis. These organisms are also of biological interest since they are able to change the morphology according to the environment where they live, through a process of reversible cell transformation, and possess structures and organelles that are not found in mammalian cells. This review analyses the process of transformation, which takes place during the life cycle of Trypanosoma cruzi in the vertebrate and invertebrate hosts. Special attention is given to the interaction of the parasite with vertebrate cells. In addition, the present knowledge of structures and organelles such as the nucleus, the plasma membrane, the sub-pellicular microtubules, the flagellum, the kinetoplast-mitochondrion complex, the peroxisome (glycosome), the acidocalcisome and the structures and organelles involved in the endocytic pathway, is reviewed from a cell biology perspective. The possible use of available data for the development of new anti parasite drugs is also discussed.

No MeSH data available.


Related in: MedlinePlus