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PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats.

Desquesnes M, Ravel S, Cuny G - Kinetoplastid Biol Dis (2002)

Bottom Line: T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice.In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony.In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification.

View Article: PubMed Central - HTML - PubMed

Affiliation: CIRDES, BP454, 01 Bobo-Dioulasso, Burkina Faso. m.desquesnes@fasonet.bf

ABSTRACT
Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas. T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice. Rats are infected principally by oral route, through contamination by flea faeces or ingestion of fleas. Trypanosoma lewisi infections in rat colonies can interfere with research protocols and fleas of wild rats are often the source of such infections. Currently, diagnosis of T. lewisi in rats is performed by microscopic observation of stained blood smears. In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony. In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification. We show that the use of ITS1 sequence of ribosomal DNA provides an efficient and sensitive assay for detection and identification of T. lewisi infection in rats and recommend the use of this assay for monitoring of T. lewisi infections in rat colonies.

No MeSH data available.


Related in: MedlinePlus

Sequence of the ITS1 of Trypanosoma lewisi (421 basis) and the product amplified by TRYP1S & TRYP1R (623 basis). Sequence of TRYP1S and Reverse sequence of TRYP1R (in bold). Sequence of the ITS1 (underlined).
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Figure 7: Sequence of the ITS1 of Trypanosoma lewisi (421 basis) and the product amplified by TRYP1S & TRYP1R (623 basis). Sequence of TRYP1S and Reverse sequence of TRYP1R (in bold). Sequence of the ITS1 (underlined).


PCR identification of Trypanosoma lewisi, a common parasite of laboratory rats.

Desquesnes M, Ravel S, Cuny G - Kinetoplastid Biol Dis (2002)

Sequence of the ITS1 of Trypanosoma lewisi (421 basis) and the product amplified by TRYP1S & TRYP1R (623 basis). Sequence of TRYP1S and Reverse sequence of TRYP1R (in bold). Sequence of the ITS1 (underlined).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC119323&req=5

Figure 7: Sequence of the ITS1 of Trypanosoma lewisi (421 basis) and the product amplified by TRYP1S & TRYP1R (623 basis). Sequence of TRYP1S and Reverse sequence of TRYP1R (in bold). Sequence of the ITS1 (underlined).
Bottom Line: T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice.In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony.In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification.

View Article: PubMed Central - HTML - PubMed

Affiliation: CIRDES, BP454, 01 Bobo-Dioulasso, Burkina Faso. m.desquesnes@fasonet.bf

ABSTRACT
Trypanosoma (Herpetosoma) lewisi is a trypanosome of the sub-genus Herpetosoma (Stercoraria section), parasite of rats (Rattus rattus and Rattus norvegicus) transmitted by fleas. T. lewisi has a stringent species specificity and cannot grow in other rodents such as mice. Rats are infected principally by oral route, through contamination by flea faeces or ingestion of fleas. Trypanosoma lewisi infections in rat colonies can interfere with research protocols and fleas of wild rats are often the source of such infections. Currently, diagnosis of T. lewisi in rats is performed by microscopic observation of stained blood smears. In the course of a research project at CIRDES, a T. lewisi infection was detected in the rat colony. In this study we evaluated PCR primer sets for their ability to diagnose multiple species of trypanosomes with a single amplification. We show that the use of ITS1 sequence of ribosomal DNA provides an efficient and sensitive assay for detection and identification of T. lewisi infection in rats and recommend the use of this assay for monitoring of T. lewisi infections in rat colonies.

No MeSH data available.


Related in: MedlinePlus