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Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients.

Dutra WO, Correa-Oliveira R, Dunne D, Cecchini LF, Fraga L, Roberts M, Soares-Silveira AM, Webster M, Yssel H, Gollob KJ - BMC Immunol. (2002)

Bottom Line: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE.Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs.However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica-Imunologia, ICB-UFMG, Belo Horizonte, MG, Brazil. waldutra@mono.icb.ufmg.br

ABSTRACT

Background: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE. Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs. Th0 in human schistosomiasis is not known. To address this question, we determined the frequency of IL-4 and IFN-gamma or IL-5 and IL-2 producing lymphocytes from schistosomiasis patients with high or low levels of IgE anti-SWAP.

Results: Our analysis showed that high and low IgE-producers responded equally to schistosomiasis antigens as determined by proliferation. Moreover, patients from both groups displayed similar percentages of circulating lymphocytes. However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers. Moreover, intracellular cytokine analysis, after short-term stimulation with anti-CD3/CD28 mAbs, showed that IgE high-producers display an increase in the percentage of T lymphocytes expressing IL-4 and IL-5 as compared to IgE low-responders. A coordinate control of the frequency of IL-4 and IL-5 producing lymphocytes in IgE high, but not IgE low-responders, was observed.

Conclusions: High IgE phenotype human schistosomiasis patients exhibit a coordinate regulation of IL-4 and IL-5 producing cells and the lymphocyte derived IL-4 comes from true polarized Th2 like cells, in the absence of measurable Th0 cells as measured by co-production of IL-4 and IFN-gamma.

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Coordinate regulation of the frequency of IL-4 and IL-5 expressing lymphocytes from high or low anti-SWAP IgE responders. The data is presented in a correlation plot considering IL-4 and IL-5 or IFN-γ and IL-2 for individual patients from the IgE low (A,C) and high (B,D) groups. The correlation coefficient was calculated using Jump Statistics program.
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Figure 5: Coordinate regulation of the frequency of IL-4 and IL-5 expressing lymphocytes from high or low anti-SWAP IgE responders. The data is presented in a correlation plot considering IL-4 and IL-5 or IFN-γ and IL-2 for individual patients from the IgE low (A,C) and high (B,D) groups. The correlation coefficient was calculated using Jump Statistics program.

Mentions: Finally, when comparing the frequency of IL-4 and IL-5 producing cells in each individual from the high responder and low responder anti-SWAP IgE group, we observed a striking difference in the coordinate control of the frequency of cytokine producing cells. As seen in figure 5, we observed a strong coordinate control (c.c. = 0.93) of the frequency of IL-4 and IL-5 producing lymphocytes only in the high IgE anti-SWAP responders. Moreover, this relationship was not seen for the frequency of cells producing IFN-γ and IL-2 (Figure 5) for either of the groups.


Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients.

Dutra WO, Correa-Oliveira R, Dunne D, Cecchini LF, Fraga L, Roberts M, Soares-Silveira AM, Webster M, Yssel H, Gollob KJ - BMC Immunol. (2002)

Coordinate regulation of the frequency of IL-4 and IL-5 expressing lymphocytes from high or low anti-SWAP IgE responders. The data is presented in a correlation plot considering IL-4 and IL-5 or IFN-γ and IL-2 for individual patients from the IgE low (A,C) and high (B,D) groups. The correlation coefficient was calculated using Jump Statistics program.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC117775&req=5

Figure 5: Coordinate regulation of the frequency of IL-4 and IL-5 expressing lymphocytes from high or low anti-SWAP IgE responders. The data is presented in a correlation plot considering IL-4 and IL-5 or IFN-γ and IL-2 for individual patients from the IgE low (A,C) and high (B,D) groups. The correlation coefficient was calculated using Jump Statistics program.
Mentions: Finally, when comparing the frequency of IL-4 and IL-5 producing cells in each individual from the high responder and low responder anti-SWAP IgE group, we observed a striking difference in the coordinate control of the frequency of cytokine producing cells. As seen in figure 5, we observed a strong coordinate control (c.c. = 0.93) of the frequency of IL-4 and IL-5 producing lymphocytes only in the high IgE anti-SWAP responders. Moreover, this relationship was not seen for the frequency of cells producing IFN-γ and IL-2 (Figure 5) for either of the groups.

Bottom Line: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE.Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs.However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica-Imunologia, ICB-UFMG, Belo Horizonte, MG, Brazil. waldutra@mono.icb.ufmg.br

ABSTRACT

Background: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE. Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs. Th0 in human schistosomiasis is not known. To address this question, we determined the frequency of IL-4 and IFN-gamma or IL-5 and IL-2 producing lymphocytes from schistosomiasis patients with high or low levels of IgE anti-SWAP.

Results: Our analysis showed that high and low IgE-producers responded equally to schistosomiasis antigens as determined by proliferation. Moreover, patients from both groups displayed similar percentages of circulating lymphocytes. However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers. Moreover, intracellular cytokine analysis, after short-term stimulation with anti-CD3/CD28 mAbs, showed that IgE high-producers display an increase in the percentage of T lymphocytes expressing IL-4 and IL-5 as compared to IgE low-responders. A coordinate control of the frequency of IL-4 and IL-5 producing lymphocytes in IgE high, but not IgE low-responders, was observed.

Conclusions: High IgE phenotype human schistosomiasis patients exhibit a coordinate regulation of IL-4 and IL-5 producing cells and the lymphocyte derived IL-4 comes from true polarized Th2 like cells, in the absence of measurable Th0 cells as measured by co-production of IL-4 and IFN-gamma.

Show MeSH
Related in: MedlinePlus