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Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients.

Dutra WO, Correa-Oliveira R, Dunne D, Cecchini LF, Fraga L, Roberts M, Soares-Silveira AM, Webster M, Yssel H, Gollob KJ - BMC Immunol. (2002)

Bottom Line: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE.Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs.However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica-Imunologia, ICB-UFMG, Belo Horizonte, MG, Brazil. waldutra@mono.icb.ufmg.br

ABSTRACT

Background: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE. Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs. Th0 in human schistosomiasis is not known. To address this question, we determined the frequency of IL-4 and IFN-gamma or IL-5 and IL-2 producing lymphocytes from schistosomiasis patients with high or low levels of IgE anti-SWAP.

Results: Our analysis showed that high and low IgE-producers responded equally to schistosomiasis antigens as determined by proliferation. Moreover, patients from both groups displayed similar percentages of circulating lymphocytes. However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers. Moreover, intracellular cytokine analysis, after short-term stimulation with anti-CD3/CD28 mAbs, showed that IgE high-producers display an increase in the percentage of T lymphocytes expressing IL-4 and IL-5 as compared to IgE low-responders. A coordinate control of the frequency of IL-4 and IL-5 producing lymphocytes in IgE high, but not IgE low-responders, was observed.

Conclusions: High IgE phenotype human schistosomiasis patients exhibit a coordinate regulation of IL-4 and IL-5 producing cells and the lymphocyte derived IL-4 comes from true polarized Th2 like cells, in the absence of measurable Th0 cells as measured by co-production of IL-4 and IFN-gamma.

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Lymphocytes from IgE high responder (n = 9) patients have a higher frequency of IL-4 and IL-5 expressing cells than the IgE low responder (n = 9) patients. Lymphocytes from the two groups of individuals were stimulated for a total of 11 h with anti-CD3, CD28, and IL-2 and stained with anti-IL-4-PE and IFN-γ-FITC or anti-IL-5-PE and IL-2-FITC as described in Materials and Methods. The data represent the mean percent of lymphocytes expressing the indicated cytokine from the two groups with their standard error. * p < 0.05 using students T test.
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Figure 4: Lymphocytes from IgE high responder (n = 9) patients have a higher frequency of IL-4 and IL-5 expressing cells than the IgE low responder (n = 9) patients. Lymphocytes from the two groups of individuals were stimulated for a total of 11 h with anti-CD3, CD28, and IL-2 and stained with anti-IL-4-PE and IFN-γ-FITC or anti-IL-5-PE and IL-2-FITC as described in Materials and Methods. The data represent the mean percent of lymphocytes expressing the indicated cytokine from the two groups with their standard error. * p < 0.05 using students T test.

Mentions: As seen in figure 4, the IgE high responder group had a significantly higher frequency of cells producing IL-4 (1.11%) and IL-5 (0.41%) after short term stimulation with anti-CD3/CD28 mAbs than did the IgE low responder group (0.31 and 0.09%, respectively). In contrast, the IgE high and low responder groups did not differ in the percentage of cells expressing IL-2 or IFN-γ (Figure 4). Non-stimulated controls showed undetectable levels of all cytokines tested (data not shown).


Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients.

Dutra WO, Correa-Oliveira R, Dunne D, Cecchini LF, Fraga L, Roberts M, Soares-Silveira AM, Webster M, Yssel H, Gollob KJ - BMC Immunol. (2002)

Lymphocytes from IgE high responder (n = 9) patients have a higher frequency of IL-4 and IL-5 expressing cells than the IgE low responder (n = 9) patients. Lymphocytes from the two groups of individuals were stimulated for a total of 11 h with anti-CD3, CD28, and IL-2 and stained with anti-IL-4-PE and IFN-γ-FITC or anti-IL-5-PE and IL-2-FITC as described in Materials and Methods. The data represent the mean percent of lymphocytes expressing the indicated cytokine from the two groups with their standard error. * p < 0.05 using students T test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC117775&req=5

Figure 4: Lymphocytes from IgE high responder (n = 9) patients have a higher frequency of IL-4 and IL-5 expressing cells than the IgE low responder (n = 9) patients. Lymphocytes from the two groups of individuals were stimulated for a total of 11 h with anti-CD3, CD28, and IL-2 and stained with anti-IL-4-PE and IFN-γ-FITC or anti-IL-5-PE and IL-2-FITC as described in Materials and Methods. The data represent the mean percent of lymphocytes expressing the indicated cytokine from the two groups with their standard error. * p < 0.05 using students T test.
Mentions: As seen in figure 4, the IgE high responder group had a significantly higher frequency of cells producing IL-4 (1.11%) and IL-5 (0.41%) after short term stimulation with anti-CD3/CD28 mAbs than did the IgE low responder group (0.31 and 0.09%, respectively). In contrast, the IgE high and low responder groups did not differ in the percentage of cells expressing IL-2 or IFN-γ (Figure 4). Non-stimulated controls showed undetectable levels of all cytokines tested (data not shown).

Bottom Line: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE.Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs.However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica-Imunologia, ICB-UFMG, Belo Horizonte, MG, Brazil. waldutra@mono.icb.ufmg.br

ABSTRACT

Background: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE. Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs. Th0 in human schistosomiasis is not known. To address this question, we determined the frequency of IL-4 and IFN-gamma or IL-5 and IL-2 producing lymphocytes from schistosomiasis patients with high or low levels of IgE anti-SWAP.

Results: Our analysis showed that high and low IgE-producers responded equally to schistosomiasis antigens as determined by proliferation. Moreover, patients from both groups displayed similar percentages of circulating lymphocytes. However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers. Moreover, intracellular cytokine analysis, after short-term stimulation with anti-CD3/CD28 mAbs, showed that IgE high-producers display an increase in the percentage of T lymphocytes expressing IL-4 and IL-5 as compared to IgE low-responders. A coordinate control of the frequency of IL-4 and IL-5 producing lymphocytes in IgE high, but not IgE low-responders, was observed.

Conclusions: High IgE phenotype human schistosomiasis patients exhibit a coordinate regulation of IL-4 and IL-5 producing cells and the lymphocyte derived IL-4 comes from true polarized Th2 like cells, in the absence of measurable Th0 cells as measured by co-production of IL-4 and IFN-gamma.

Show MeSH
Related in: MedlinePlus