Limits...
Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients.

Dutra WO, Correa-Oliveira R, Dunne D, Cecchini LF, Fraga L, Roberts M, Soares-Silveira AM, Webster M, Yssel H, Gollob KJ - BMC Immunol. (2002)

Bottom Line: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE.Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs.However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica-Imunologia, ICB-UFMG, Belo Horizonte, MG, Brazil. waldutra@mono.icb.ufmg.br

ABSTRACT

Background: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE. Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs. Th0 in human schistosomiasis is not known. To address this question, we determined the frequency of IL-4 and IFN-gamma or IL-5 and IL-2 producing lymphocytes from schistosomiasis patients with high or low levels of IgE anti-SWAP.

Results: Our analysis showed that high and low IgE-producers responded equally to schistosomiasis antigens as determined by proliferation. Moreover, patients from both groups displayed similar percentages of circulating lymphocytes. However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers. Moreover, intracellular cytokine analysis, after short-term stimulation with anti-CD3/CD28 mAbs, showed that IgE high-producers display an increase in the percentage of T lymphocytes expressing IL-4 and IL-5 as compared to IgE low-responders. A coordinate control of the frequency of IL-4 and IL-5 producing lymphocytes in IgE high, but not IgE low-responders, was observed.

Conclusions: High IgE phenotype human schistosomiasis patients exhibit a coordinate regulation of IL-4 and IL-5 producing cells and the lymphocyte derived IL-4 comes from true polarized Th2 like cells, in the absence of measurable Th0 cells as measured by co-production of IL-4 and IFN-gamma.

Show MeSH

Related in: MedlinePlus

Ex vivo lymphocyte profile using flow cytometry revealed a higher percent of activated CD4+ T cells in the IgE high responder group. Lymphocytes freshly isolated from whole blood were stained for the indicated markers and the percent of cells in the lymphocyte gate expressing each of the shown markers are diagrammed as mean with the standard error. * p < 0.05 using the students T test.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC117775&req=5

Figure 2: Ex vivo lymphocyte profile using flow cytometry revealed a higher percent of activated CD4+ T cells in the IgE high responder group. Lymphocytes freshly isolated from whole blood were stained for the indicated markers and the percent of cells in the lymphocyte gate expressing each of the shown markers are diagrammed as mean with the standard error. * p < 0.05 using the students T test.

Mentions: Lymphocytes from patients of both groups were compared for percentage of cells expressing CD4, CD8, CD19, and for co-expression of the activation marker HLA-DR in the CD4+ and CD8+ T cell populations. Figure 2 demonstrates that the percentage of CD4+, CD8+, or CD19+ cells were not different between the two groups. However, the percentage of activated CD4+ T cells was significantly higher in the IgE high responder group.


Polarized Th2 like cells, in the absence of Th0 cells, are responsible for lymphocyte produced IL-4 in high IgE-producer schistosomiasis patients.

Dutra WO, Correa-Oliveira R, Dunne D, Cecchini LF, Fraga L, Roberts M, Soares-Silveira AM, Webster M, Yssel H, Gollob KJ - BMC Immunol. (2002)

Ex vivo lymphocyte profile using flow cytometry revealed a higher percent of activated CD4+ T cells in the IgE high responder group. Lymphocytes freshly isolated from whole blood were stained for the indicated markers and the percent of cells in the lymphocyte gate expressing each of the shown markers are diagrammed as mean with the standard error. * p < 0.05 using the students T test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC117775&req=5

Figure 2: Ex vivo lymphocyte profile using flow cytometry revealed a higher percent of activated CD4+ T cells in the IgE high responder group. Lymphocytes freshly isolated from whole blood were stained for the indicated markers and the percent of cells in the lymphocyte gate expressing each of the shown markers are diagrammed as mean with the standard error. * p < 0.05 using the students T test.
Mentions: Lymphocytes from patients of both groups were compared for percentage of cells expressing CD4, CD8, CD19, and for co-expression of the activation marker HLA-DR in the CD4+ and CD8+ T cell populations. Figure 2 demonstrates that the percentage of CD4+, CD8+, or CD19+ cells were not different between the two groups. However, the percentage of activated CD4+ T cells was significantly higher in the IgE high responder group.

Bottom Line: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE.Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs.However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Departamento de Bioquímica-Imunologia, ICB-UFMG, Belo Horizonte, MG, Brazil. waldutra@mono.icb.ufmg.br

ABSTRACT

Background: Human resistance to re-infection with S. mansoni is correlated with high levels of anti-soluble adult worm antigens (SWAP) IgE. Although it has been shown that IL-4 and IL-5 are crucial in establishing IgE responses in vitro, the active in vivo production of these cytokines by T cells, and the degree of polarization of Th2 vs. Th0 in human schistosomiasis is not known. To address this question, we determined the frequency of IL-4 and IFN-gamma or IL-5 and IL-2 producing lymphocytes from schistosomiasis patients with high or low levels of IgE anti-SWAP.

Results: Our analysis showed that high and low IgE-producers responded equally to schistosomiasis antigens as determined by proliferation. Moreover, patients from both groups displayed similar percentages of circulating lymphocytes. However, high IgE-producers had an increased percentage of activated CD4+ T cells as compared to the low IgE-producers. Moreover, intracellular cytokine analysis, after short-term stimulation with anti-CD3/CD28 mAbs, showed that IgE high-producers display an increase in the percentage of T lymphocytes expressing IL-4 and IL-5 as compared to IgE low-responders. A coordinate control of the frequency of IL-4 and IL-5 producing lymphocytes in IgE high, but not IgE low-responders, was observed.

Conclusions: High IgE phenotype human schistosomiasis patients exhibit a coordinate regulation of IL-4 and IL-5 producing cells and the lymphocyte derived IL-4 comes from true polarized Th2 like cells, in the absence of measurable Th0 cells as measured by co-production of IL-4 and IFN-gamma.

Show MeSH
Related in: MedlinePlus