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Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses.

Nadelhaft I, Miranda-Sousa AJ, Vera PL - BMC Neurosci (2002)

Bottom Line: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate.This difference also persists in the brain.In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veteran's Administration Medical Center, Bay Pines, FL 33744, USA. inadelha@hsc.usf.edu

ABSTRACT

Background: This work examines the central nervous system distribution of virus-labeled neurons from the rat urinary bladder and the prostate simultaneously within the same tissue sections. Two immunohistochemically distinct pseudorabies virus strains were simultaneously injected into male Sprague Dawley rats (approximately 280 gm). One virus was injected into the bladder and the other into the prostate. After incubation intervals of 2.25, 2.5, 2.75, 3 and 4 days, sections from the spinal cord and brain were processed immunohistochemically to detect cells, within a single section, which were labeled separately by each virus or were labeled by both viruses.

Results: Each strain of virus labeled a separate population of neurons and some neurons were labeled by both strains. The majority of neurons labeled by virus from the urinary bladder were found in the L6-S1 spinal cord segments within the dorsal gray commissure, the intermediolateral area and the superficial dorsal horn. Neurons labeled by virus from the prostate were mainly found in the L1-L2 spinal cord segments in the dorsal gray commissure and the intermediolateral areas. Double-labeled interneurons in L1-L2 were mainly located in the intermediolateral area. In L6-S1 they were divided between the dorsal gray commissure and the intermediolateral area.

Conclusions: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate. This difference also persists in the brain. In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

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Neurons labeled from either the bladder or the prostate three days post infection Maps of sections from the lumbosacral spinal cord showing neurons labeled from either the bladder or the prostate three days post infection. Neurons labeled by virus that infected the bladder are red ("x" or "o") and those that are labeled by virus that infected the prostate are green ("x" or "o"). The "o" means that the neuron is also cholinergic (preganglionic). Blue neurons ("x") are cholinergic neurons that are not labeled by either virus. A: Section from the L6-S1 spinal cord. Cholinergic bladder neurons (parasympathetic preganglionic) are restricted to the intermediolateral area. Bladder neurons that are not cholinergic are found in the IML and DGC (interneurons) and in SDH. Cholinergic neurons that are not labeled by either virus (blue "x") are found in the ventral horn (somatic motor neurons), the intermediolateral area (preganglionic motor neurons that innervate other visceral organs) and around the central canal. Note that, in this particular section, there are no neurons labeled by virus that was injected into the prostate. B: Section from the L1-L2 spinal cord. Note that there are neurons labelled by virus that infected the bladder as well as the prostate. There are cholinergic (preganglionic, located in the intermediolateral and commissural areas) and non-cholinergic neurons labeled by virus from both organs. Bar: 1 mm.
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Figure 5: Neurons labeled from either the bladder or the prostate three days post infection Maps of sections from the lumbosacral spinal cord showing neurons labeled from either the bladder or the prostate three days post infection. Neurons labeled by virus that infected the bladder are red ("x" or "o") and those that are labeled by virus that infected the prostate are green ("x" or "o"). The "o" means that the neuron is also cholinergic (preganglionic). Blue neurons ("x") are cholinergic neurons that are not labeled by either virus. A: Section from the L6-S1 spinal cord. Cholinergic bladder neurons (parasympathetic preganglionic) are restricted to the intermediolateral area. Bladder neurons that are not cholinergic are found in the IML and DGC (interneurons) and in SDH. Cholinergic neurons that are not labeled by either virus (blue "x") are found in the ventral horn (somatic motor neurons), the intermediolateral area (preganglionic motor neurons that innervate other visceral organs) and around the central canal. Note that, in this particular section, there are no neurons labeled by virus that was injected into the prostate. B: Section from the L1-L2 spinal cord. Note that there are neurons labelled by virus that infected the bladder as well as the prostate. There are cholinergic (preganglionic, located in the intermediolateral and commissural areas) and non-cholinergic neurons labeled by virus from both organs. Bar: 1 mm.

Mentions: 3) Neurons located in the superficial dorsal horn (475) were only labeled from injections into the bladder and were only found in L6-S1 (Figure 5A) and only after 2.75 days.


Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses.

Nadelhaft I, Miranda-Sousa AJ, Vera PL - BMC Neurosci (2002)

Neurons labeled from either the bladder or the prostate three days post infection Maps of sections from the lumbosacral spinal cord showing neurons labeled from either the bladder or the prostate three days post infection. Neurons labeled by virus that infected the bladder are red ("x" or "o") and those that are labeled by virus that infected the prostate are green ("x" or "o"). The "o" means that the neuron is also cholinergic (preganglionic). Blue neurons ("x") are cholinergic neurons that are not labeled by either virus. A: Section from the L6-S1 spinal cord. Cholinergic bladder neurons (parasympathetic preganglionic) are restricted to the intermediolateral area. Bladder neurons that are not cholinergic are found in the IML and DGC (interneurons) and in SDH. Cholinergic neurons that are not labeled by either virus (blue "x") are found in the ventral horn (somatic motor neurons), the intermediolateral area (preganglionic motor neurons that innervate other visceral organs) and around the central canal. Note that, in this particular section, there are no neurons labeled by virus that was injected into the prostate. B: Section from the L1-L2 spinal cord. Note that there are neurons labelled by virus that infected the bladder as well as the prostate. There are cholinergic (preganglionic, located in the intermediolateral and commissural areas) and non-cholinergic neurons labeled by virus from both organs. Bar: 1 mm.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC117599&req=5

Figure 5: Neurons labeled from either the bladder or the prostate three days post infection Maps of sections from the lumbosacral spinal cord showing neurons labeled from either the bladder or the prostate three days post infection. Neurons labeled by virus that infected the bladder are red ("x" or "o") and those that are labeled by virus that infected the prostate are green ("x" or "o"). The "o" means that the neuron is also cholinergic (preganglionic). Blue neurons ("x") are cholinergic neurons that are not labeled by either virus. A: Section from the L6-S1 spinal cord. Cholinergic bladder neurons (parasympathetic preganglionic) are restricted to the intermediolateral area. Bladder neurons that are not cholinergic are found in the IML and DGC (interneurons) and in SDH. Cholinergic neurons that are not labeled by either virus (blue "x") are found in the ventral horn (somatic motor neurons), the intermediolateral area (preganglionic motor neurons that innervate other visceral organs) and around the central canal. Note that, in this particular section, there are no neurons labeled by virus that was injected into the prostate. B: Section from the L1-L2 spinal cord. Note that there are neurons labelled by virus that infected the bladder as well as the prostate. There are cholinergic (preganglionic, located in the intermediolateral and commissural areas) and non-cholinergic neurons labeled by virus from both organs. Bar: 1 mm.
Mentions: 3) Neurons located in the superficial dorsal horn (475) were only labeled from injections into the bladder and were only found in L6-S1 (Figure 5A) and only after 2.75 days.

Bottom Line: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate.This difference also persists in the brain.In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veteran's Administration Medical Center, Bay Pines, FL 33744, USA. inadelha@hsc.usf.edu

ABSTRACT

Background: This work examines the central nervous system distribution of virus-labeled neurons from the rat urinary bladder and the prostate simultaneously within the same tissue sections. Two immunohistochemically distinct pseudorabies virus strains were simultaneously injected into male Sprague Dawley rats (approximately 280 gm). One virus was injected into the bladder and the other into the prostate. After incubation intervals of 2.25, 2.5, 2.75, 3 and 4 days, sections from the spinal cord and brain were processed immunohistochemically to detect cells, within a single section, which were labeled separately by each virus or were labeled by both viruses.

Results: Each strain of virus labeled a separate population of neurons and some neurons were labeled by both strains. The majority of neurons labeled by virus from the urinary bladder were found in the L6-S1 spinal cord segments within the dorsal gray commissure, the intermediolateral area and the superficial dorsal horn. Neurons labeled by virus from the prostate were mainly found in the L1-L2 spinal cord segments in the dorsal gray commissure and the intermediolateral areas. Double-labeled interneurons in L1-L2 were mainly located in the intermediolateral area. In L6-S1 they were divided between the dorsal gray commissure and the intermediolateral area.

Conclusions: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate. This difference also persists in the brain. In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

Show MeSH
Related in: MedlinePlus