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Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses.

Nadelhaft I, Miranda-Sousa AJ, Vera PL - BMC Neurosci (2002)

Bottom Line: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate.This difference also persists in the brain.In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veteran's Administration Medical Center, Bay Pines, FL 33744, USA. inadelha@hsc.usf.edu

ABSTRACT

Background: This work examines the central nervous system distribution of virus-labeled neurons from the rat urinary bladder and the prostate simultaneously within the same tissue sections. Two immunohistochemically distinct pseudorabies virus strains were simultaneously injected into male Sprague Dawley rats (approximately 280 gm). One virus was injected into the bladder and the other into the prostate. After incubation intervals of 2.25, 2.5, 2.75, 3 and 4 days, sections from the spinal cord and brain were processed immunohistochemically to detect cells, within a single section, which were labeled separately by each virus or were labeled by both viruses.

Results: Each strain of virus labeled a separate population of neurons and some neurons were labeled by both strains. The majority of neurons labeled by virus from the urinary bladder were found in the L6-S1 spinal cord segments within the dorsal gray commissure, the intermediolateral area and the superficial dorsal horn. Neurons labeled by virus from the prostate were mainly found in the L1-L2 spinal cord segments in the dorsal gray commissure and the intermediolateral areas. Double-labeled interneurons in L1-L2 were mainly located in the intermediolateral area. In L6-S1 they were divided between the dorsal gray commissure and the intermediolateral area.

Conclusions: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate. This difference also persists in the brain. In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

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Types of neurons in the L1-L2 spinal cord segments as a function of incubation time The number of neurons of each type and at each location in the spinal cord section increases with incubation time. Virus from the prostate (types P, PChAT and DBL) labels the majority of the neurons. Cholinergic prostate neurons (PChAT) are located in the dorsal gray commissure and the intermediolateral area. Their number is approximately constant as a function of time. Cholinergic bladder neurons (BchAT) are found only after three days incubation and these are mainly located in the intermediolateral area. After three days, large numbers of prostate (P) and bladder (B) non-cholinergic neurons (presumably interneurons) are found in the dorsal gray commissure and the intermediolateral area. Double-labeled neurons are confined to the dorsal gray commissure.
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Figure 3: Types of neurons in the L1-L2 spinal cord segments as a function of incubation time The number of neurons of each type and at each location in the spinal cord section increases with incubation time. Virus from the prostate (types P, PChAT and DBL) labels the majority of the neurons. Cholinergic prostate neurons (PChAT) are located in the dorsal gray commissure and the intermediolateral area. Their number is approximately constant as a function of time. Cholinergic bladder neurons (BchAT) are found only after three days incubation and these are mainly located in the intermediolateral area. After three days, large numbers of prostate (P) and bladder (B) non-cholinergic neurons (presumably interneurons) are found in the dorsal gray commissure and the intermediolateral area. Double-labeled neurons are confined to the dorsal gray commissure.

Mentions: Virus-labeled neurons in lumbosacral spinal cord first appeared 2.25 days post-infection. Table 1 and Figures 2, 3 and 4 display the means and standard errors of numbers of labeled neurons found in the L1-L2 and L6-S1 segments of the spinal cord as a function of incubation time. The table and figures demonstrate the following points:


Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses.

Nadelhaft I, Miranda-Sousa AJ, Vera PL - BMC Neurosci (2002)

Types of neurons in the L1-L2 spinal cord segments as a function of incubation time The number of neurons of each type and at each location in the spinal cord section increases with incubation time. Virus from the prostate (types P, PChAT and DBL) labels the majority of the neurons. Cholinergic prostate neurons (PChAT) are located in the dorsal gray commissure and the intermediolateral area. Their number is approximately constant as a function of time. Cholinergic bladder neurons (BchAT) are found only after three days incubation and these are mainly located in the intermediolateral area. After three days, large numbers of prostate (P) and bladder (B) non-cholinergic neurons (presumably interneurons) are found in the dorsal gray commissure and the intermediolateral area. Double-labeled neurons are confined to the dorsal gray commissure.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC117599&req=5

Figure 3: Types of neurons in the L1-L2 spinal cord segments as a function of incubation time The number of neurons of each type and at each location in the spinal cord section increases with incubation time. Virus from the prostate (types P, PChAT and DBL) labels the majority of the neurons. Cholinergic prostate neurons (PChAT) are located in the dorsal gray commissure and the intermediolateral area. Their number is approximately constant as a function of time. Cholinergic bladder neurons (BchAT) are found only after three days incubation and these are mainly located in the intermediolateral area. After three days, large numbers of prostate (P) and bladder (B) non-cholinergic neurons (presumably interneurons) are found in the dorsal gray commissure and the intermediolateral area. Double-labeled neurons are confined to the dorsal gray commissure.
Mentions: Virus-labeled neurons in lumbosacral spinal cord first appeared 2.25 days post-infection. Table 1 and Figures 2, 3 and 4 display the means and standard errors of numbers of labeled neurons found in the L1-L2 and L6-S1 segments of the spinal cord as a function of incubation time. The table and figures demonstrate the following points:

Bottom Line: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate.This difference also persists in the brain.In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

View Article: PubMed Central - HTML - PubMed

Affiliation: Veteran's Administration Medical Center, Bay Pines, FL 33744, USA. inadelha@hsc.usf.edu

ABSTRACT

Background: This work examines the central nervous system distribution of virus-labeled neurons from the rat urinary bladder and the prostate simultaneously within the same tissue sections. Two immunohistochemically distinct pseudorabies virus strains were simultaneously injected into male Sprague Dawley rats (approximately 280 gm). One virus was injected into the bladder and the other into the prostate. After incubation intervals of 2.25, 2.5, 2.75, 3 and 4 days, sections from the spinal cord and brain were processed immunohistochemically to detect cells, within a single section, which were labeled separately by each virus or were labeled by both viruses.

Results: Each strain of virus labeled a separate population of neurons and some neurons were labeled by both strains. The majority of neurons labeled by virus from the urinary bladder were found in the L6-S1 spinal cord segments within the dorsal gray commissure, the intermediolateral area and the superficial dorsal horn. Neurons labeled by virus from the prostate were mainly found in the L1-L2 spinal cord segments in the dorsal gray commissure and the intermediolateral areas. Double-labeled interneurons in L1-L2 were mainly located in the intermediolateral area. In L6-S1 they were divided between the dorsal gray commissure and the intermediolateral area.

Conclusions: Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate. This difference also persists in the brain. In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.

Show MeSH
Related in: MedlinePlus