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Expansion of antibody reactivity in the cerebrospinal fluid of multiple sclerosis patients - follow-up and clinical implications.

Petereit HF, Reske D - Cerebrospinal Fluid Res (2005)

Bottom Line: Serum and cerebrospinal fluid sample pairs were obtained from 1 to 5 consecutive lumbar punctures.Results were analysed with regard to clinical characteristics of the patients.Furthermore, an intrathecal measles antibody production was associated with a rapid progression of the disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurology, University of Cologne, Kerpener Str, 62, D-50924 Cologne, Germany. hela.petereit@medizin.uni-koeln.de

ABSTRACT

Background: An intrathecal polyspecific antibody response is a well known finding in multiple sclerosis. However, little is known about the evolution of intrathecal antibodies over time and their impact on the disease progress. Therefore, we focused in this study on the intrathecal polyspecific antibody response in multiple sclerosis.

Methods: Here we present a follow-up study of 70 patients with multiple sclerosis over 1 to 106 months. Serum and cerebrospinal fluid sample pairs were obtained from 1 to 5 consecutive lumbar punctures. CSF cell count, the IgG index, local IgG synthesis, oligoclonal bands and the antibody index for measles, rubella or varicella zoster were calculated. Results were analysed with regard to clinical characteristics of the patients.

Results: Once an intrathecal antibody response was established, it persisted. De novo antibody response against measles virus developed in 7% of the patients between the first and the second spinal tap. In two of seven patients where 5 consecutive CSF samples were available, the intrathecal antibody response expanded from one to three antigens. Furthermore, an intrathecal measles antibody production was associated with a rapid progression of the disease.

Conclusion: These data stress the importance of activated B cells for the disease process and the clinical outcome in multiple sclerosis.

No MeSH data available.


Related in: MedlinePlus

Percentage of patients with an intrathecal antibody synthesis against measles, rubella, and varicella zoster virus at five successive spinal taps (LP, n = 70 for LP 1 and 2, n = 26 for LP 3, n = 12 for LP4 and n = 7 for LP5). There was a significant increase (p <0.05) in patients positive for antibody synthesis against measles virus over time. There were no significant changes in rubella or varicella zoster antibody synthesis.
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Figure 1: Percentage of patients with an intrathecal antibody synthesis against measles, rubella, and varicella zoster virus at five successive spinal taps (LP, n = 70 for LP 1 and 2, n = 26 for LP 3, n = 12 for LP4 and n = 7 for LP5). There was a significant increase (p <0.05) in patients positive for antibody synthesis against measles virus over time. There were no significant changes in rubella or varicella zoster antibody synthesis.

Mentions: As expected, the presence of OCB in the CSF was more sensitive in the detection of an intrathecal IgG production than a positive IgG index [22,23]. For instance, at the 1st LP 94% of patients had positive oligoclonal CSF bands whereas 88% of patients had a positive IgG index. Once positive the OCB bands remained positive during the follow-up. The percentage of patients who were positive for oligoclonal bands in the CSF increased after the 1st LP from 94 to 100 %. Likewise, the relative number of patients with an intrathecal antibody synthesis against measles virus increased significantly over time (Figure 1). No obvious change in rubella and varicella zoster antibody positive patients over time was observed. This significant increase in patients with an intrathecal antibody production against measles was seen in both patient subgroups irrespective of whether or not they were treated with any immunomodulatory treatment mentioned above at the 2nd LP or not (Table 2).


Expansion of antibody reactivity in the cerebrospinal fluid of multiple sclerosis patients - follow-up and clinical implications.

Petereit HF, Reske D - Cerebrospinal Fluid Res (2005)

Percentage of patients with an intrathecal antibody synthesis against measles, rubella, and varicella zoster virus at five successive spinal taps (LP, n = 70 for LP 1 and 2, n = 26 for LP 3, n = 12 for LP4 and n = 7 for LP5). There was a significant increase (p <0.05) in patients positive for antibody synthesis against measles virus over time. There were no significant changes in rubella or varicella zoster antibody synthesis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1173126&req=5

Figure 1: Percentage of patients with an intrathecal antibody synthesis against measles, rubella, and varicella zoster virus at five successive spinal taps (LP, n = 70 for LP 1 and 2, n = 26 for LP 3, n = 12 for LP4 and n = 7 for LP5). There was a significant increase (p <0.05) in patients positive for antibody synthesis against measles virus over time. There were no significant changes in rubella or varicella zoster antibody synthesis.
Mentions: As expected, the presence of OCB in the CSF was more sensitive in the detection of an intrathecal IgG production than a positive IgG index [22,23]. For instance, at the 1st LP 94% of patients had positive oligoclonal CSF bands whereas 88% of patients had a positive IgG index. Once positive the OCB bands remained positive during the follow-up. The percentage of patients who were positive for oligoclonal bands in the CSF increased after the 1st LP from 94 to 100 %. Likewise, the relative number of patients with an intrathecal antibody synthesis against measles virus increased significantly over time (Figure 1). No obvious change in rubella and varicella zoster antibody positive patients over time was observed. This significant increase in patients with an intrathecal antibody production against measles was seen in both patient subgroups irrespective of whether or not they were treated with any immunomodulatory treatment mentioned above at the 2nd LP or not (Table 2).

Bottom Line: Serum and cerebrospinal fluid sample pairs were obtained from 1 to 5 consecutive lumbar punctures.Results were analysed with regard to clinical characteristics of the patients.Furthermore, an intrathecal measles antibody production was associated with a rapid progression of the disease.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Neurology, University of Cologne, Kerpener Str, 62, D-50924 Cologne, Germany. hela.petereit@medizin.uni-koeln.de

ABSTRACT

Background: An intrathecal polyspecific antibody response is a well known finding in multiple sclerosis. However, little is known about the evolution of intrathecal antibodies over time and their impact on the disease progress. Therefore, we focused in this study on the intrathecal polyspecific antibody response in multiple sclerosis.

Methods: Here we present a follow-up study of 70 patients with multiple sclerosis over 1 to 106 months. Serum and cerebrospinal fluid sample pairs were obtained from 1 to 5 consecutive lumbar punctures. CSF cell count, the IgG index, local IgG synthesis, oligoclonal bands and the antibody index for measles, rubella or varicella zoster were calculated. Results were analysed with regard to clinical characteristics of the patients.

Results: Once an intrathecal antibody response was established, it persisted. De novo antibody response against measles virus developed in 7% of the patients between the first and the second spinal tap. In two of seven patients where 5 consecutive CSF samples were available, the intrathecal antibody response expanded from one to three antigens. Furthermore, an intrathecal measles antibody production was associated with a rapid progression of the disease.

Conclusion: These data stress the importance of activated B cells for the disease process and the clinical outcome in multiple sclerosis.

No MeSH data available.


Related in: MedlinePlus