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Somatic Embryogenesis, Rhizogenesis, and Morphinan Alkaloids Production in Two Species of Opium Poppy.

Kassem MA, Jacquin A - J. Biomed. Biotechnol. (2001)

Bottom Line: For rhizogenesis, the most important response is on the cotyledons and leaves in these two species.Morphine was only detected in aerial parts of Papaver somniferum album.These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
A study of somatic embryogenesis and rhizogenesis and their influence on production of morphinan alkaloids on two species of opium poppy is presented. We identified the ratios of auxin and cytokinin that caused somatic embryogenesis and rhizogenesis in hypocotyl and cotyledons of Papaver somniferum album and Papaver orientale splendidissimum. The hypocotyls and cotyledons both show somatic embryogenesis in Papaver somniferum album whereas only the cotyledons were embryogenic in Papaver orientale splendidissimum. For rhizogenesis, the most important response is on the cotyledons and leaves in these two species. Histology showed characteristic stages of somatic embryo: Globular, cotyledonous, and heart cotyledonary. High performance liquid chromatography analysis showed that the roots of both species synthesized codeine, thebaine, and papaverine. Morphine was only detected in aerial parts of Papaver somniferum album. Codeine and thebaine were detected in the rhizogenous but no embryonic callus. These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

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Somatic embryogenesis obtained from roots, cotyledons, and hypocotyls of Papaver somniferum album, with two plant growth regulator combinations (NAA/kinetin = 0.2/0.1 and 0.5/0.1 mg/l), after fourmonths of culture.
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Figure 3: Somatic embryogenesis obtained from roots, cotyledons, and hypocotyls of Papaver somniferum album, with two plant growth regulator combinations (NAA/kinetin = 0.2/0.1 and 0.5/0.1 mg/l), after fourmonths of culture.

Mentions: We observed that after four months of culture, cotyledon was the explant that gave the highest percentages of somatic embryos, 16% with NAA/kinetin = 0.2/0.1 mg/l and 15% with NAA/kinetin = 0.5/0.1 mg/l. Embryogenesis from hypocotyl occurs in 5% of explants with NAA/kinetin = 0.2/0.1 mg/l and 1.5% with NAA/kinetin = 0.5/0.1 mg/l. Roots did not produce any somatic embryos. For both cotyledons and hypocotyls NAA/kinetin = 0.2/0.1 mg/l and 0.5/0.1 mg/l were the optimal plant growth regulator combinations (Figure 3).


Somatic Embryogenesis, Rhizogenesis, and Morphinan Alkaloids Production in Two Species of Opium Poppy.

Kassem MA, Jacquin A - J. Biomed. Biotechnol. (2001)

Somatic embryogenesis obtained from roots, cotyledons, and hypocotyls of Papaver somniferum album, with two plant growth regulator combinations (NAA/kinetin = 0.2/0.1 and 0.5/0.1 mg/l), after fourmonths of culture.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC113780&req=5

Figure 3: Somatic embryogenesis obtained from roots, cotyledons, and hypocotyls of Papaver somniferum album, with two plant growth regulator combinations (NAA/kinetin = 0.2/0.1 and 0.5/0.1 mg/l), after fourmonths of culture.
Mentions: We observed that after four months of culture, cotyledon was the explant that gave the highest percentages of somatic embryos, 16% with NAA/kinetin = 0.2/0.1 mg/l and 15% with NAA/kinetin = 0.5/0.1 mg/l. Embryogenesis from hypocotyl occurs in 5% of explants with NAA/kinetin = 0.2/0.1 mg/l and 1.5% with NAA/kinetin = 0.5/0.1 mg/l. Roots did not produce any somatic embryos. For both cotyledons and hypocotyls NAA/kinetin = 0.2/0.1 mg/l and 0.5/0.1 mg/l were the optimal plant growth regulator combinations (Figure 3).

Bottom Line: For rhizogenesis, the most important response is on the cotyledons and leaves in these two species.Morphine was only detected in aerial parts of Papaver somniferum album.These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
A study of somatic embryogenesis and rhizogenesis and their influence on production of morphinan alkaloids on two species of opium poppy is presented. We identified the ratios of auxin and cytokinin that caused somatic embryogenesis and rhizogenesis in hypocotyl and cotyledons of Papaver somniferum album and Papaver orientale splendidissimum. The hypocotyls and cotyledons both show somatic embryogenesis in Papaver somniferum album whereas only the cotyledons were embryogenic in Papaver orientale splendidissimum. For rhizogenesis, the most important response is on the cotyledons and leaves in these two species. Histology showed characteristic stages of somatic embryo: Globular, cotyledonous, and heart cotyledonary. High performance liquid chromatography analysis showed that the roots of both species synthesized codeine, thebaine, and papaverine. Morphine was only detected in aerial parts of Papaver somniferum album. Codeine and thebaine were detected in the rhizogenous but no embryonic callus. These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

No MeSH data available.


Related in: MedlinePlus