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Somatic Embryogenesis, Rhizogenesis, and Morphinan Alkaloids Production in Two Species of Opium Poppy.

Kassem MA, Jacquin A - J. Biomed. Biotechnol. (2001)

Bottom Line: For rhizogenesis, the most important response is on the cotyledons and leaves in these two species.Morphine was only detected in aerial parts of Papaver somniferum album.These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
A study of somatic embryogenesis and rhizogenesis and their influence on production of morphinan alkaloids on two species of opium poppy is presented. We identified the ratios of auxin and cytokinin that caused somatic embryogenesis and rhizogenesis in hypocotyl and cotyledons of Papaver somniferum album and Papaver orientale splendidissimum. The hypocotyls and cotyledons both show somatic embryogenesis in Papaver somniferum album whereas only the cotyledons were embryogenic in Papaver orientale splendidissimum. For rhizogenesis, the most important response is on the cotyledons and leaves in these two species. Histology showed characteristic stages of somatic embryo: Globular, cotyledonous, and heart cotyledonary. High performance liquid chromatography analysis showed that the roots of both species synthesized codeine, thebaine, and papaverine. Morphine was only detected in aerial parts of Papaver somniferum album. Codeine and thebaine were detected in the rhizogenous but no embryonic callus. These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

No MeSH data available.


Related in: MedlinePlus

Somatic embryogenesis from a cell suspension of Papaver somniferum album cultivated on liquid media S1 (2,4-D 0.2 mg/l and kinetin 0.1 mg/l) and S1PGRF (plant growth regulators free). NAA, naphtaleneacetic acid; BA, benzyladenine; LS, Linsmaier and Skoog medium.
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Figure 1: Somatic embryogenesis from a cell suspension of Papaver somniferum album cultivated on liquid media S1 (2,4-D 0.2 mg/l and kinetin 0.1 mg/l) and S1PGRF (plant growth regulators free). NAA, naphtaleneacetic acid; BA, benzyladenine; LS, Linsmaier and Skoog medium.

Mentions: We observed that 14.5% of explants derived from callus grown inliquid medium S1HF gave somatic embryo and 18% of somaticproembryo when transferred to solid medium LS-NAA/BA-S30. When transferred to solid medium LS-NAA/BA-S60, however, callus derived from liquid medium S1 produced no somatic embryos but only 2.5% of somatic proembryos after transfer to solid media LS-NAA/BA-S30 and LS-NAA/BA-S60. We also observed that callus derived from S1HF and from S1 produced no embryonic structures when transferred to medium LS-S30 without plant growth regulators (Figure 1). The presence of some small proembryos on solid medium LS-NAA/BA-S30 after transfer of callus from themedium S1 (containing 2,4-D and kinetin) confirmed that removal from plant growth regulators improves somatic embryogenesis.


Somatic Embryogenesis, Rhizogenesis, and Morphinan Alkaloids Production in Two Species of Opium Poppy.

Kassem MA, Jacquin A - J. Biomed. Biotechnol. (2001)

Somatic embryogenesis from a cell suspension of Papaver somniferum album cultivated on liquid media S1 (2,4-D 0.2 mg/l and kinetin 0.1 mg/l) and S1PGRF (plant growth regulators free). NAA, naphtaleneacetic acid; BA, benzyladenine; LS, Linsmaier and Skoog medium.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC113780&req=5

Figure 1: Somatic embryogenesis from a cell suspension of Papaver somniferum album cultivated on liquid media S1 (2,4-D 0.2 mg/l and kinetin 0.1 mg/l) and S1PGRF (plant growth regulators free). NAA, naphtaleneacetic acid; BA, benzyladenine; LS, Linsmaier and Skoog medium.
Mentions: We observed that 14.5% of explants derived from callus grown inliquid medium S1HF gave somatic embryo and 18% of somaticproembryo when transferred to solid medium LS-NAA/BA-S30. When transferred to solid medium LS-NAA/BA-S60, however, callus derived from liquid medium S1 produced no somatic embryos but only 2.5% of somatic proembryos after transfer to solid media LS-NAA/BA-S30 and LS-NAA/BA-S60. We also observed that callus derived from S1HF and from S1 produced no embryonic structures when transferred to medium LS-S30 without plant growth regulators (Figure 1). The presence of some small proembryos on solid medium LS-NAA/BA-S30 after transfer of callus from themedium S1 (containing 2,4-D and kinetin) confirmed that removal from plant growth regulators improves somatic embryogenesis.

Bottom Line: For rhizogenesis, the most important response is on the cotyledons and leaves in these two species.Morphine was only detected in aerial parts of Papaver somniferum album.These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
A study of somatic embryogenesis and rhizogenesis and their influence on production of morphinan alkaloids on two species of opium poppy is presented. We identified the ratios of auxin and cytokinin that caused somatic embryogenesis and rhizogenesis in hypocotyl and cotyledons of Papaver somniferum album and Papaver orientale splendidissimum. The hypocotyls and cotyledons both show somatic embryogenesis in Papaver somniferum album whereas only the cotyledons were embryogenic in Papaver orientale splendidissimum. For rhizogenesis, the most important response is on the cotyledons and leaves in these two species. Histology showed characteristic stages of somatic embryo: Globular, cotyledonous, and heart cotyledonary. High performance liquid chromatography analysis showed that the roots of both species synthesized codeine, thebaine, and papaverine. Morphine was only detected in aerial parts of Papaver somniferum album. Codeine and thebaine were detected in the rhizogenous but no embryonic callus. These results suggest that root organogenesis is causally related to alkaloid biosynthesis.

No MeSH data available.


Related in: MedlinePlus