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Response of Escherichia coli Containing Mycobacterial Carotene Genes to UV Radiation.

Houssaini-Iraqui M, Khamlichi N, El Yamani J, Rastogi N - J. Biomed. Biotechnol. (2001)

Bottom Line: The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned.After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51).E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

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ABSTRACT
The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

No MeSH data available.


Related in: MedlinePlus

Susceptibility of various E. coli strains to UV radiations. Symbols; 1, E. coli; 2, E. coli containing pC51; 3, E. coli containing pHLD69; d, distance separating the bacterial culture from the UV lamp.
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Figure 4: Susceptibility of various E. coli strains to UV radiations. Symbols; 1, E. coli; 2, E. coli containing pC51; 3, E. coli containing pHLD69; d, distance separating the bacterial culture from the UV lamp.

Mentions: To analyze the role of the carotenoids in protection against UVirradiation, E. coli (pC51) was exposed to UV rays as described in materials and methods section. Briefly, strains were incubated at 37°C for 60 h prior to UV exposure. As indicated in Figure 3, this incubation time was intentionally chosen as it permits the maximal synthesis of the carotenes. At a distance of 14 or 50 cm from the UV lamp, all strains had the same sensitivity to the UV light (Figure 4). However, at a distance of 77 cm, cells of E. coli (pC51) were more resistant than the parallel controls (E. coli and E. coli containing pHLD69; Figure 4). This result indicates that the carotenoid pigments are able to prevent the lethal action of UV irradiation on bacterial cells.


Response of Escherichia coli Containing Mycobacterial Carotene Genes to UV Radiation.

Houssaini-Iraqui M, Khamlichi N, El Yamani J, Rastogi N - J. Biomed. Biotechnol. (2001)

Susceptibility of various E. coli strains to UV radiations. Symbols; 1, E. coli; 2, E. coli containing pC51; 3, E. coli containing pHLD69; d, distance separating the bacterial culture from the UV lamp.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC113779&req=5

Figure 4: Susceptibility of various E. coli strains to UV radiations. Symbols; 1, E. coli; 2, E. coli containing pC51; 3, E. coli containing pHLD69; d, distance separating the bacterial culture from the UV lamp.
Mentions: To analyze the role of the carotenoids in protection against UVirradiation, E. coli (pC51) was exposed to UV rays as described in materials and methods section. Briefly, strains were incubated at 37°C for 60 h prior to UV exposure. As indicated in Figure 3, this incubation time was intentionally chosen as it permits the maximal synthesis of the carotenes. At a distance of 14 or 50 cm from the UV lamp, all strains had the same sensitivity to the UV light (Figure 4). However, at a distance of 77 cm, cells of E. coli (pC51) were more resistant than the parallel controls (E. coli and E. coli containing pHLD69; Figure 4). This result indicates that the carotenoid pigments are able to prevent the lethal action of UV irradiation on bacterial cells.

Bottom Line: The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned.After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51).E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

No MeSH data available.


Related in: MedlinePlus