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Response of Escherichia coli Containing Mycobacterial Carotene Genes to UV Radiation.

Houssaini-Iraqui M, Khamlichi N, El Yamani J, Rastogi N - J. Biomed. Biotechnol. (2001)

Bottom Line: The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned.After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51).E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

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ABSTRACT
The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

No MeSH data available.


Related in: MedlinePlus

The spectrum (a) and concentrations (b) of pigments extracted from E. coli containing pC51. Symbols; 1, lycopene; 2, neurosporene.
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Figure 3: The spectrum (a) and concentrations (b) of pigments extracted from E. coli containing pC51. Symbols; 1, lycopene; 2, neurosporene.

Mentions: Indeed, this spectrum was similar to both the commercial lycopene and also the lycopene extracted from the wild-type M. aurum A+ (Figure 3). The identity of this major pigment was also verified by cochromatography with authentic sample. E. coli harboring plasmid pC51 accumulated large amounts of lycopene; its yield was 0.25 μg/mg dry wt after 60 h of incubation at 37°C (Figure 3). Furthermore, a yellow pigment was isolated and was confirmed to be neurosporene by its spectrum (Figure 3). This pigment is present in similar amount as compared to lycopene as it is converted to lycopene (Figure 3). However, phytoene, phytofluene and ζ-carotene (Figure 1) were not detected in extract of E. coli transformed by pC51.


Response of Escherichia coli Containing Mycobacterial Carotene Genes to UV Radiation.

Houssaini-Iraqui M, Khamlichi N, El Yamani J, Rastogi N - J. Biomed. Biotechnol. (2001)

The spectrum (a) and concentrations (b) of pigments extracted from E. coli containing pC51. Symbols; 1, lycopene; 2, neurosporene.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC113779&req=5

Figure 3: The spectrum (a) and concentrations (b) of pigments extracted from E. coli containing pC51. Symbols; 1, lycopene; 2, neurosporene.
Mentions: Indeed, this spectrum was similar to both the commercial lycopene and also the lycopene extracted from the wild-type M. aurum A+ (Figure 3). The identity of this major pigment was also verified by cochromatography with authentic sample. E. coli harboring plasmid pC51 accumulated large amounts of lycopene; its yield was 0.25 μg/mg dry wt after 60 h of incubation at 37°C (Figure 3). Furthermore, a yellow pigment was isolated and was confirmed to be neurosporene by its spectrum (Figure 3). This pigment is present in similar amount as compared to lycopene as it is converted to lycopene (Figure 3). However, phytoene, phytofluene and ζ-carotene (Figure 1) were not detected in extract of E. coli transformed by pC51.

Bottom Line: The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned.After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51).E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

No MeSH data available.


Related in: MedlinePlus