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Response of Escherichia coli Containing Mycobacterial Carotene Genes to UV Radiation.

Houssaini-Iraqui M, Khamlichi N, El Yamani J, Rastogi N - J. Biomed. Biotechnol. (2001)

Bottom Line: The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned.After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51).E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

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ABSTRACT
The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

No MeSH data available.


Related in: MedlinePlus

Pathway of lycopene biosynthesis in non-photosyntheticbacteria [2, 4, 13, 14]. Symbols; IPP, isopentenyl pyrophosphate; FPP, farnesyl pyrophosphate; GGPP, geranylgeranyl pyrophosphate.
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Figure 1: Pathway of lycopene biosynthesis in non-photosyntheticbacteria [2, 4, 13, 14]. Symbols; IPP, isopentenyl pyrophosphate; FPP, farnesyl pyrophosphate; GGPP, geranylgeranyl pyrophosphate.

Mentions: The synthesis of lycopene can be divided into two stages: the synthesis of phytoene and its desaturation (dehydrogenation) [11]. The precursor of carotene is isopentenyl pyrophosphate (IPP, C5) obtained from mevalonic acid. IPP is converted to farnesyl pyrophosphate (FPP, C15). The condensation of IPP and FPP are required for the synthesis of the geranylgeranyl pyrophosphate (GGPP, C20). This reaction is catalyzed by GGPP synthase. The gene coding for this enzyme has been cloned in several species [2, 4, 6, 12]. The dimerization of GGPP produces prephytoene pyrphosphate (PPPP, C40) that is converted into phytoene (C40 colorless hydrocarbon). These reactions are catalysed by phytoene synthase [13]. The gene coding for this enzyme was cloned in Erwinia herbicola and leads to the synthesis phytoene from GGPP in Escherichia coli [2, 4, 14]. Four sequential desaturations are required to convert phytoene into lycopene by phytoene desaturase (dehydrogenase); the intermediate precursors are successively phytofluene, ζ-carotene and neurosporene (Figure 1) [11, 13]. In non-photosynthetic organisms such as Erwinia herbicola and Erwinia uredorora, phytoene desaturase is the product of a single gene that is capable to express in E. coli [2, 4, 13, 15].


Response of Escherichia coli Containing Mycobacterial Carotene Genes to UV Radiation.

Houssaini-Iraqui M, Khamlichi N, El Yamani J, Rastogi N - J. Biomed. Biotechnol. (2001)

Pathway of lycopene biosynthesis in non-photosyntheticbacteria [2, 4, 13, 14]. Symbols; IPP, isopentenyl pyrophosphate; FPP, farnesyl pyrophosphate; GGPP, geranylgeranyl pyrophosphate.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC113779&req=5

Figure 1: Pathway of lycopene biosynthesis in non-photosyntheticbacteria [2, 4, 13, 14]. Symbols; IPP, isopentenyl pyrophosphate; FPP, farnesyl pyrophosphate; GGPP, geranylgeranyl pyrophosphate.
Mentions: The synthesis of lycopene can be divided into two stages: the synthesis of phytoene and its desaturation (dehydrogenation) [11]. The precursor of carotene is isopentenyl pyrophosphate (IPP, C5) obtained from mevalonic acid. IPP is converted to farnesyl pyrophosphate (FPP, C15). The condensation of IPP and FPP are required for the synthesis of the geranylgeranyl pyrophosphate (GGPP, C20). This reaction is catalyzed by GGPP synthase. The gene coding for this enzyme has been cloned in several species [2, 4, 6, 12]. The dimerization of GGPP produces prephytoene pyrphosphate (PPPP, C40) that is converted into phytoene (C40 colorless hydrocarbon). These reactions are catalysed by phytoene synthase [13]. The gene coding for this enzyme was cloned in Erwinia herbicola and leads to the synthesis phytoene from GGPP in Escherichia coli [2, 4, 14]. Four sequential desaturations are required to convert phytoene into lycopene by phytoene desaturase (dehydrogenase); the intermediate precursors are successively phytofluene, ζ-carotene and neurosporene (Figure 1) [11, 13]. In non-photosynthetic organisms such as Erwinia herbicola and Erwinia uredorora, phytoene desaturase is the product of a single gene that is capable to express in E. coli [2, 4, 13, 15].

Bottom Line: The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned.After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51).E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

View Article: PubMed Central - HTML - PubMed

ABSTRACT
The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.

No MeSH data available.


Related in: MedlinePlus