Limits...
Effects of dexamethasone on TNF-alpha-induced release of cytokines from purified human blood eosinophils.

Uings I, Puxeddu I, Temkin V, Smith SJ, Fattah D, Ray KP, Levi-Schaffer F - Clin Mol Allergy (2005)

Bottom Line: METHODS: The effect of dexamethasone on TNF-alpha induced eosinophils survival, degranulation (ECP), cytokines release (IL-8, GM-CSF) and adhesion to VCAM-1, ICAM-1 and IgG coated wells (EPO release) were evaluated.RESULTS: The drug inhibited IL-8 and GM-CSF production, but not viability, degranulation or adhesion in human peripheral blood eosinophils.CONCLUSION: These results indicate that part of the activity of glucocorticosteroids on eosinophils may be mediated by their ability to inhibit cytokine secretion that in turn is important for the perpetuation of the allergic inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel. fls@cc.huji.ac.il.

ABSTRACT
SUMMARY: BACKGROUND: TNF-alpha is an important mediator in allergy also for its effects on eosinophils. METHODS: The effect of dexamethasone on TNF-alpha induced eosinophils survival, degranulation (ECP), cytokines release (IL-8, GM-CSF) and adhesion to VCAM-1, ICAM-1 and IgG coated wells (EPO release) were evaluated. RESULTS: The drug inhibited IL-8 and GM-CSF production, but not viability, degranulation or adhesion in human peripheral blood eosinophils. CONCLUSION: These results indicate that part of the activity of glucocorticosteroids on eosinophils may be mediated by their ability to inhibit cytokine secretion that in turn is important for the perpetuation of the allergic inflammation.

No MeSH data available.


Related in: MedlinePlus

Effect of TNF-α on eosinophil viability in vitro. Eosinophils were incubated with different concentrations of TNF-α for 22 hrs. Eosinophil viability was evaluated by Trypan blue exclusion test. Values are expressed as percentage of survival increase in the presence of TNF-α vs medium alone. Values are mean ± SEM (n = 14).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC1131914&req=5

Figure 1: Effect of TNF-α on eosinophil viability in vitro. Eosinophils were incubated with different concentrations of TNF-α for 22 hrs. Eosinophil viability was evaluated by Trypan blue exclusion test. Values are expressed as percentage of survival increase in the presence of TNF-α vs medium alone. Values are mean ± SEM (n = 14).

Mentions: TNF-α significantly increased eosinophil viability in a concentration-dependent fashion, compared to culture in medium alone, with a maximal effect at 20 ng/ml (Figure 1). This effect was not influenced by the addition of dexamethasone in the culture medium (13.3% vs 11.5%).


Effects of dexamethasone on TNF-alpha-induced release of cytokines from purified human blood eosinophils.

Uings I, Puxeddu I, Temkin V, Smith SJ, Fattah D, Ray KP, Levi-Schaffer F - Clin Mol Allergy (2005)

Effect of TNF-α on eosinophil viability in vitro. Eosinophils were incubated with different concentrations of TNF-α for 22 hrs. Eosinophil viability was evaluated by Trypan blue exclusion test. Values are expressed as percentage of survival increase in the presence of TNF-α vs medium alone. Values are mean ± SEM (n = 14).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC1131914&req=5

Figure 1: Effect of TNF-α on eosinophil viability in vitro. Eosinophils were incubated with different concentrations of TNF-α for 22 hrs. Eosinophil viability was evaluated by Trypan blue exclusion test. Values are expressed as percentage of survival increase in the presence of TNF-α vs medium alone. Values are mean ± SEM (n = 14).
Mentions: TNF-α significantly increased eosinophil viability in a concentration-dependent fashion, compared to culture in medium alone, with a maximal effect at 20 ng/ml (Figure 1). This effect was not influenced by the addition of dexamethasone in the culture medium (13.3% vs 11.5%).

Bottom Line: METHODS: The effect of dexamethasone on TNF-alpha induced eosinophils survival, degranulation (ECP), cytokines release (IL-8, GM-CSF) and adhesion to VCAM-1, ICAM-1 and IgG coated wells (EPO release) were evaluated.RESULTS: The drug inhibited IL-8 and GM-CSF production, but not viability, degranulation or adhesion in human peripheral blood eosinophils.CONCLUSION: These results indicate that part of the activity of glucocorticosteroids on eosinophils may be mediated by their ability to inhibit cytokine secretion that in turn is important for the perpetuation of the allergic inflammation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacology, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel. fls@cc.huji.ac.il.

ABSTRACT
SUMMARY: BACKGROUND: TNF-alpha is an important mediator in allergy also for its effects on eosinophils. METHODS: The effect of dexamethasone on TNF-alpha induced eosinophils survival, degranulation (ECP), cytokines release (IL-8, GM-CSF) and adhesion to VCAM-1, ICAM-1 and IgG coated wells (EPO release) were evaluated. RESULTS: The drug inhibited IL-8 and GM-CSF production, but not viability, degranulation or adhesion in human peripheral blood eosinophils. CONCLUSION: These results indicate that part of the activity of glucocorticosteroids on eosinophils may be mediated by their ability to inhibit cytokine secretion that in turn is important for the perpetuation of the allergic inflammation.

No MeSH data available.


Related in: MedlinePlus