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The antimicrobial susceptibility of Stenotrophomonas maltophilia isolates using three different methods and their genetic relatedness.

Tatman-Otkun M, Gürcan S, Ozer B, Aydoslu B, Bukavaz S - BMC Microbiol. (2005)

Bottom Line: Although trimethoprim/sulfamethoxazole (SXT) and ciprofloxacin have the lowest MIC values, SXT (98.1%) and ticarcillin/clavulanate (TLc) (73.1%) were found to be the most effective antimicrobials by agar dilution method, which was in accordance with the breakpoints established by NCCLS.By using the NCCLS criteria, disc diffusion and E-test were unreliable alternative methods for S. maltophilia, except for SXT.However, the significance of these data should be confirmed by further experimental and clinical studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Medical Faculty, Department of Microbiology and Clinical Microbiology, Trakya University, Turkey. otkunm@trakya.edu.tr

ABSTRACT

Background: Stenotrophomonas maltophilia is inherently resistant to many antimicrobials. So far, antimicrobial susceptibility tests for S. maltophilia have not been fully standardized. The purpose of the study was to compare the susceptibility of S. maltophilia isolates against seven different antimicrobials using three different methods and to investigate their genetic relatedness.

Results: Although trimethoprim/sulfamethoxazole (SXT) and ciprofloxacin have the lowest MIC values, SXT (98.1%) and ticarcillin/clavulanate (TLc) (73.1%) were found to be the most effective antimicrobials by agar dilution method, which was in accordance with the breakpoints established by NCCLS. Disc diffusion and E-test was in agreement with agar dilution method for SXT. When the isolation dates, clinics, antibiotyping, and AP-PCR data were investigated, two small outbreaks consisting of five and three cases were determined.

Conclusion: By using the NCCLS criteria, disc diffusion and E-test were unreliable alternative methods for S. maltophilia, except for SXT. However, the significance of these data should be confirmed by further experimental and clinical studies.

Show MeSH
AP-PCR profiles of S. maltophilia strins including statistical analysis and dendrogram showing the genetic relationship between strains.
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Figure 1: AP-PCR profiles of S. maltophilia strins including statistical analysis and dendrogram showing the genetic relationship between strains.

Mentions: A total of 44 different patterns of 52 strains were obtained by The arbitrarily primed PCR (AP-PCR). Three small clusters were observed. All of five strains in pattern l were isolated in the Neurology clinic between March and September 2002. All three strains in pattern ll were isolated in the Paediatrics clinic between January and March 2001, and three strains in pattern lll were isolated in the Paediatrics, Nephrology and Chest Diseases clinics between January and November 2001. The antimicrobial susceptibility results supported the AP-PCR method for patterns l and ll, but not for pattern III. The strains in pattern I were resistant to ceftazidime (CAZ), cefepime (CPM), piperacillin (PIP) and piperacillin/tazobactam (PTZ), and susceptible to TLc, CIP, and SXT. The strains in pattern II were susceptible to all antimicrobial except for CIP. The dendrogram showed a Dice similarity coefficient ranging from14.8 to 100% (Figure 1).


The antimicrobial susceptibility of Stenotrophomonas maltophilia isolates using three different methods and their genetic relatedness.

Tatman-Otkun M, Gürcan S, Ozer B, Aydoslu B, Bukavaz S - BMC Microbiol. (2005)

AP-PCR profiles of S. maltophilia strins including statistical analysis and dendrogram showing the genetic relationship between strains.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1131904&req=5

Figure 1: AP-PCR profiles of S. maltophilia strins including statistical analysis and dendrogram showing the genetic relationship between strains.
Mentions: A total of 44 different patterns of 52 strains were obtained by The arbitrarily primed PCR (AP-PCR). Three small clusters were observed. All of five strains in pattern l were isolated in the Neurology clinic between March and September 2002. All three strains in pattern ll were isolated in the Paediatrics clinic between January and March 2001, and three strains in pattern lll were isolated in the Paediatrics, Nephrology and Chest Diseases clinics between January and November 2001. The antimicrobial susceptibility results supported the AP-PCR method for patterns l and ll, but not for pattern III. The strains in pattern I were resistant to ceftazidime (CAZ), cefepime (CPM), piperacillin (PIP) and piperacillin/tazobactam (PTZ), and susceptible to TLc, CIP, and SXT. The strains in pattern II were susceptible to all antimicrobial except for CIP. The dendrogram showed a Dice similarity coefficient ranging from14.8 to 100% (Figure 1).

Bottom Line: Although trimethoprim/sulfamethoxazole (SXT) and ciprofloxacin have the lowest MIC values, SXT (98.1%) and ticarcillin/clavulanate (TLc) (73.1%) were found to be the most effective antimicrobials by agar dilution method, which was in accordance with the breakpoints established by NCCLS.By using the NCCLS criteria, disc diffusion and E-test were unreliable alternative methods for S. maltophilia, except for SXT.However, the significance of these data should be confirmed by further experimental and clinical studies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Medical Faculty, Department of Microbiology and Clinical Microbiology, Trakya University, Turkey. otkunm@trakya.edu.tr

ABSTRACT

Background: Stenotrophomonas maltophilia is inherently resistant to many antimicrobials. So far, antimicrobial susceptibility tests for S. maltophilia have not been fully standardized. The purpose of the study was to compare the susceptibility of S. maltophilia isolates against seven different antimicrobials using three different methods and to investigate their genetic relatedness.

Results: Although trimethoprim/sulfamethoxazole (SXT) and ciprofloxacin have the lowest MIC values, SXT (98.1%) and ticarcillin/clavulanate (TLc) (73.1%) were found to be the most effective antimicrobials by agar dilution method, which was in accordance with the breakpoints established by NCCLS. Disc diffusion and E-test was in agreement with agar dilution method for SXT. When the isolation dates, clinics, antibiotyping, and AP-PCR data were investigated, two small outbreaks consisting of five and three cases were determined.

Conclusion: By using the NCCLS criteria, disc diffusion and E-test were unreliable alternative methods for S. maltophilia, except for SXT. However, the significance of these data should be confirmed by further experimental and clinical studies.

Show MeSH