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Combined antibiotic and free radical trap treatment is effective at combating Staphylococcus-aureus-induced septic arthritis.

Sakiniene E, Collins LV - Arthritis Res. (2002)

Bottom Line: Although early antibiotic treatment of patients with septic arthritis eradicates bacteria, joint destruction commonly results from the unregulated host inflammatory responses to infection.The spin trap compound phenyl-N-tert-butyl nitrone (PBN) has been shown to have both anti-inflammatory and antioxidant effects.The aim of this study was to assess the effect of combined systemic administration of PBN and cloxacillin on the development of Staphylococcus aureus arthritis.Three days after Naval Medical Research Institute (NMRI) mice were infected intravenously with S. aureus LS-1, daily treatment was started with cloxacillin alone, PBN alone, or cloxacillin and PBN.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology, University of Göteborg, Sweden. Egidija.Sakiniene@rheuma.gu.se

ABSTRACT
Although early antibiotic treatment of patients with septic arthritis eradicates bacteria, joint destruction commonly results from the unregulated host inflammatory responses to infection. The spin trap compound phenyl-N-tert-butyl nitrone (PBN) has been shown to have both anti-inflammatory and antioxidant effects. The aim of this study was to assess the effect of combined systemic administration of PBN and cloxacillin on the development of Staphylococcus aureus arthritis.Three days after Naval Medical Research Institute (NMRI) mice were infected intravenously with S. aureus LS-1, daily treatment was started with cloxacillin alone, PBN alone, or cloxacillin and PBN. Arthritis, weight loss and general condition were evaluated for each mouse, and joints were analyzed histopathologically. Systemic administration of PBN in conjunction with cloxacillin ameliorated the course of experimental S. aureus arthritis, as evidenced by an increased cure rate. Thus, combinatorial antioxidant plus antibiotic anti-inflammatory therapies represent a potentially efficacious approach to the management of septic arthritis.

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The effects of different treatments on the number of bacteria isolated from the kidneys of NMRI mice, 10 days after they were inoculated intravenously with S. aureus strain LS-1 (n = 9–19). **P < 0.005; NS, not significant (*P < 0.05). CFU, colony-forming units; CLOX, cloxacillin; PBN, phenyl-N-tert-butyl nitrone.
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Figure 4: The effects of different treatments on the number of bacteria isolated from the kidneys of NMRI mice, 10 days after they were inoculated intravenously with S. aureus strain LS-1 (n = 9–19). **P < 0.005; NS, not significant (*P < 0.05). CFU, colony-forming units; CLOX, cloxacillin; PBN, phenyl-N-tert-butyl nitrone.

Mentions: All of the control and PBN-alone treated mice harbored bacteria in the kidneys 10 days after infection, compared to 58% of mice treated with combined PBN-cloxacillin therapy, and 68% of mice treated with cloxacillin alone. The numbers of bacteria in kidneys were significantly lower in the combined PBN-cloxacillin and cloxacillin-alone groups than in the control or PBN-alone treatment groups (Fig. 4).


Combined antibiotic and free radical trap treatment is effective at combating Staphylococcus-aureus-induced septic arthritis.

Sakiniene E, Collins LV - Arthritis Res. (2002)

The effects of different treatments on the number of bacteria isolated from the kidneys of NMRI mice, 10 days after they were inoculated intravenously with S. aureus strain LS-1 (n = 9–19). **P < 0.005; NS, not significant (*P < 0.05). CFU, colony-forming units; CLOX, cloxacillin; PBN, phenyl-N-tert-butyl nitrone.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC111022&req=5

Figure 4: The effects of different treatments on the number of bacteria isolated from the kidneys of NMRI mice, 10 days after they were inoculated intravenously with S. aureus strain LS-1 (n = 9–19). **P < 0.005; NS, not significant (*P < 0.05). CFU, colony-forming units; CLOX, cloxacillin; PBN, phenyl-N-tert-butyl nitrone.
Mentions: All of the control and PBN-alone treated mice harbored bacteria in the kidneys 10 days after infection, compared to 58% of mice treated with combined PBN-cloxacillin therapy, and 68% of mice treated with cloxacillin alone. The numbers of bacteria in kidneys were significantly lower in the combined PBN-cloxacillin and cloxacillin-alone groups than in the control or PBN-alone treatment groups (Fig. 4).

Bottom Line: Although early antibiotic treatment of patients with septic arthritis eradicates bacteria, joint destruction commonly results from the unregulated host inflammatory responses to infection.The spin trap compound phenyl-N-tert-butyl nitrone (PBN) has been shown to have both anti-inflammatory and antioxidant effects.The aim of this study was to assess the effect of combined systemic administration of PBN and cloxacillin on the development of Staphylococcus aureus arthritis.Three days after Naval Medical Research Institute (NMRI) mice were infected intravenously with S. aureus LS-1, daily treatment was started with cloxacillin alone, PBN alone, or cloxacillin and PBN.

View Article: PubMed Central - PubMed

Affiliation: Department of Rheumatology, University of Göteborg, Sweden. Egidija.Sakiniene@rheuma.gu.se

ABSTRACT
Although early antibiotic treatment of patients with septic arthritis eradicates bacteria, joint destruction commonly results from the unregulated host inflammatory responses to infection. The spin trap compound phenyl-N-tert-butyl nitrone (PBN) has been shown to have both anti-inflammatory and antioxidant effects. The aim of this study was to assess the effect of combined systemic administration of PBN and cloxacillin on the development of Staphylococcus aureus arthritis.Three days after Naval Medical Research Institute (NMRI) mice were infected intravenously with S. aureus LS-1, daily treatment was started with cloxacillin alone, PBN alone, or cloxacillin and PBN. Arthritis, weight loss and general condition were evaluated for each mouse, and joints were analyzed histopathologically. Systemic administration of PBN in conjunction with cloxacillin ameliorated the course of experimental S. aureus arthritis, as evidenced by an increased cure rate. Thus, combinatorial antioxidant plus antibiotic anti-inflammatory therapies represent a potentially efficacious approach to the management of septic arthritis.

Show MeSH
Related in: MedlinePlus