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Anti-tumorigenic and Pro-apoptotic effects of CKBM on gastric cancer growth in nude mice.

Shin VY, So WH, Liu ES, Wu YJ, Pang SF, Cho CH - Int J Med Sci (2004)

Bottom Line: Results showed that higher doses of CKBM (0.4 or 0.8 ml/mouse/day) produced a dose-dependent inhibitory effect on gastric tumor growth after 28-day drug treatment.This was associated with a decrease of cellular proliferation by 30% with concomitant increase in apoptosis by 97% in gastric tumor cells when compared with the control group.In contrast, CKBM showed no effect on angiogenesis in gastric tumors.

View Article: PubMed Central - PubMed

Affiliation: 1 Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, Hong Kong, HKSAR, China.

ABSTRACT
Natural botanical products can be integrated with western medicine to optimize the treatment outcome, increase immune function and minimize the side effects from western drug treatment. CKBM is a combination of herbs and yeasts formulated based on traditional Chinese medicinal principles. Previous study has demonstrated that CKBM is capable of improving immune responsiveness through the induction of cytokine mediators, such as TNF-alpha and IL-6. In this study, we aimed to investigate the effect of this immunomodulatory drug on gastric cancer growth using a human xenograft model. Gastric cancer tissues were implanted subcutaneously into athymic nude mice followed by a 14-day or 28-day of CKBM treatment. Results showed that higher doses of CKBM (0.4 or 0.8 ml/mouse/day) produced a dose-dependent inhibitory effect on gastric tumor growth after 28-day drug treatment. This was associated with a decrease of cellular proliferation by 30% with concomitant increase in apoptosis by 97% in gastric tumor cells when compared with the control group. In contrast, CKBM showed no effect on angiogenesis in gastric tumors. This study demonstrates the anti-tumorigenic action of CKBM on gastric cancer probably via inhibition of cell proliferation and induction of apoptosis, and provides future potential targets of this drug candidate on cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on gastric tumor growth in Balb/c nude mice (*P<0.05 when compared with the corresponding control group). Values are means ± SEM of 10-14 mice per group.
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Figure 1: Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on gastric tumor growth in Balb/c nude mice (*P<0.05 when compared with the corresponding control group). Values are means ± SEM of 10-14 mice per group.

Mentions: All the control (water vehicle-treated) and CKBM-treated mice developed subcutaneous tumor after gastric cancer tissue implantation. Figures 1A & 1B show the effect of CKBM on tumor growth 14 days or 28 days after treatment. The tumor growth of the control group increased steadily during the 14- and 28-day experimental periods. Results showed that CKBM exhibited a dose-dependent inhibition on tumor growth starting from Day 7 onwards. There was a significant 50% reduction on tumor growth in the CKBM-treated groups at doses of 0.4 and 0.8 ml/mouse on Day 21 and Day 28 after drug treatment (Figure 1B) when compared with the corresponding control group. CKBM did not affect the body weight changes in these animals during the experimental period.


Anti-tumorigenic and Pro-apoptotic effects of CKBM on gastric cancer growth in nude mice.

Shin VY, So WH, Liu ES, Wu YJ, Pang SF, Cho CH - Int J Med Sci (2004)

Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on gastric tumor growth in Balb/c nude mice (*P<0.05 when compared with the corresponding control group). Values are means ± SEM of 10-14 mice per group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1074708&req=5

Figure 1: Effect of CKBM treatment (0.2, 0.4 or 0.8 ml/mouse, given i.g. once daily) for (A) 14 days; (B) 28 days on gastric tumor growth in Balb/c nude mice (*P<0.05 when compared with the corresponding control group). Values are means ± SEM of 10-14 mice per group.
Mentions: All the control (water vehicle-treated) and CKBM-treated mice developed subcutaneous tumor after gastric cancer tissue implantation. Figures 1A & 1B show the effect of CKBM on tumor growth 14 days or 28 days after treatment. The tumor growth of the control group increased steadily during the 14- and 28-day experimental periods. Results showed that CKBM exhibited a dose-dependent inhibition on tumor growth starting from Day 7 onwards. There was a significant 50% reduction on tumor growth in the CKBM-treated groups at doses of 0.4 and 0.8 ml/mouse on Day 21 and Day 28 after drug treatment (Figure 1B) when compared with the corresponding control group. CKBM did not affect the body weight changes in these animals during the experimental period.

Bottom Line: Results showed that higher doses of CKBM (0.4 or 0.8 ml/mouse/day) produced a dose-dependent inhibitory effect on gastric tumor growth after 28-day drug treatment.This was associated with a decrease of cellular proliferation by 30% with concomitant increase in apoptosis by 97% in gastric tumor cells when compared with the control group.In contrast, CKBM showed no effect on angiogenesis in gastric tumors.

View Article: PubMed Central - PubMed

Affiliation: 1 Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, Hong Kong, HKSAR, China.

ABSTRACT
Natural botanical products can be integrated with western medicine to optimize the treatment outcome, increase immune function and minimize the side effects from western drug treatment. CKBM is a combination of herbs and yeasts formulated based on traditional Chinese medicinal principles. Previous study has demonstrated that CKBM is capable of improving immune responsiveness through the induction of cytokine mediators, such as TNF-alpha and IL-6. In this study, we aimed to investigate the effect of this immunomodulatory drug on gastric cancer growth using a human xenograft model. Gastric cancer tissues were implanted subcutaneously into athymic nude mice followed by a 14-day or 28-day of CKBM treatment. Results showed that higher doses of CKBM (0.4 or 0.8 ml/mouse/day) produced a dose-dependent inhibitory effect on gastric tumor growth after 28-day drug treatment. This was associated with a decrease of cellular proliferation by 30% with concomitant increase in apoptosis by 97% in gastric tumor cells when compared with the control group. In contrast, CKBM showed no effect on angiogenesis in gastric tumors. This study demonstrates the anti-tumorigenic action of CKBM on gastric cancer probably via inhibition of cell proliferation and induction of apoptosis, and provides future potential targets of this drug candidate on cancer therapy.

No MeSH data available.


Related in: MedlinePlus