Limits...
Roots of Withania somnifera Inhibit Forestomach and Skin Carcinogenesis in Mice.

Padmavathi B, Rath PC, Rao AR, Singh RP - Evid Based Complement Alternat Med (2005)

Bottom Line: We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model.Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver.Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

View Article: PubMed Central - PubMed

ABSTRACT
We evaluated the cancer chemopreventive efficacy of the Withania somnifera root, which has been used in the Indian traditional medicine system for many centuries for the treatment of various ailments. Since, studies showing its mechanism-based cancer chemopreventive efficacy are limited, this was investigated in the present study. We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model. Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver. Further, in a long-term tumorigenesis study, Withania inhibited benzo(a)pyrene-induced forestomach papillomagenesis, showing up to 60 and 92% inhibition in tumor incidence and multiplicity, respectively. Similarly, Withania inhibited 7,12-dimethylbenzanthracene-induced skin papillomagenesis, showing up to 45 and 71% inhibition in tumor incidence and multiplicity. In both studies, Withania showed no apparent toxic effects in mice as monitored by the body weight gain profile. Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

No MeSH data available.


Related in: MedlinePlus

Histopathology of tumor samples from forestomach and skin papillomagenesis studies. Tissues samples were harvested at the end of each experiment and processed for hematoxylin and eosin staining as mentioned in Methods. A representative picture is shown for (A) forestomach tumor and (B) skin tumor at 200× magnification in each case.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC1062157&req=5

fig7: Histopathology of tumor samples from forestomach and skin papillomagenesis studies. Tissues samples were harvested at the end of each experiment and processed for hematoxylin and eosin staining as mentioned in Methods. A representative picture is shown for (A) forestomach tumor and (B) skin tumor at 200× magnification in each case.

Mentions: During forestomach papillomagenesis, we observed that dietary feeding of Withania root did not produce any decease in body weight gain (Fig. 4A) or diet consumption. The control group treated with B(a)P alone showed a 100% incidence of forestomach papillomas (% of mice bearing tumors) at the end of 180 days (Fig. 4B). Estimation of the number of papillomas showed 11.1 ± 2.68 papillomas/animal in the control group (Fig. 4C). The mice fed with the 2.5% Withania diet for 6 weeks [2 weeks before, 4 weeks during and 2 weeks after B(a)P treatment] showed only 40% tumor incidence (Fig. 4B), and a strong and significant (P < 0.001) decrease in mean number of papillomas/animal, which was only 0.89 ± 1.08 (Fig. 4C). Though the tumor incidence was 87% in the HD group, the mean number of papillomas/animal was significantly (P < 0.001) reduced to only 2.61 ± 1.72 (Fig. 4C). We also observed a slight increase in the body weight of the mice fed with Withania diets compared with the control group, indicating no apparent side effects or toxicity in the mice (Fig. 4A). Further, forestomach tumors (Fig. 7A) were processed for histopathological evaluation by hematoxylin and eosin staining, showing that keratinized pearl was more prevalent in tumors of the control group of mice, representing an advanced stage in tumor development of epithelial origin. Together, these results showed that Withania root has preventive efficacy against carcinogen-induced forestomach papillomagenesis, and the lower dose used in the present study showed slightly more preventive efficacy as compared with a higher dose in this regard.


Roots of Withania somnifera Inhibit Forestomach and Skin Carcinogenesis in Mice.

Padmavathi B, Rath PC, Rao AR, Singh RP - Evid Based Complement Alternat Med (2005)

Histopathology of tumor samples from forestomach and skin papillomagenesis studies. Tissues samples were harvested at the end of each experiment and processed for hematoxylin and eosin staining as mentioned in Methods. A representative picture is shown for (A) forestomach tumor and (B) skin tumor at 200× magnification in each case.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1062157&req=5

fig7: Histopathology of tumor samples from forestomach and skin papillomagenesis studies. Tissues samples were harvested at the end of each experiment and processed for hematoxylin and eosin staining as mentioned in Methods. A representative picture is shown for (A) forestomach tumor and (B) skin tumor at 200× magnification in each case.
Mentions: During forestomach papillomagenesis, we observed that dietary feeding of Withania root did not produce any decease in body weight gain (Fig. 4A) or diet consumption. The control group treated with B(a)P alone showed a 100% incidence of forestomach papillomas (% of mice bearing tumors) at the end of 180 days (Fig. 4B). Estimation of the number of papillomas showed 11.1 ± 2.68 papillomas/animal in the control group (Fig. 4C). The mice fed with the 2.5% Withania diet for 6 weeks [2 weeks before, 4 weeks during and 2 weeks after B(a)P treatment] showed only 40% tumor incidence (Fig. 4B), and a strong and significant (P < 0.001) decrease in mean number of papillomas/animal, which was only 0.89 ± 1.08 (Fig. 4C). Though the tumor incidence was 87% in the HD group, the mean number of papillomas/animal was significantly (P < 0.001) reduced to only 2.61 ± 1.72 (Fig. 4C). We also observed a slight increase in the body weight of the mice fed with Withania diets compared with the control group, indicating no apparent side effects or toxicity in the mice (Fig. 4A). Further, forestomach tumors (Fig. 7A) were processed for histopathological evaluation by hematoxylin and eosin staining, showing that keratinized pearl was more prevalent in tumors of the control group of mice, representing an advanced stage in tumor development of epithelial origin. Together, these results showed that Withania root has preventive efficacy against carcinogen-induced forestomach papillomagenesis, and the lower dose used in the present study showed slightly more preventive efficacy as compared with a higher dose in this regard.

Bottom Line: We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model.Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver.Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

View Article: PubMed Central - PubMed

ABSTRACT
We evaluated the cancer chemopreventive efficacy of the Withania somnifera root, which has been used in the Indian traditional medicine system for many centuries for the treatment of various ailments. Since, studies showing its mechanism-based cancer chemopreventive efficacy are limited, this was investigated in the present study. We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model. Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver. Further, in a long-term tumorigenesis study, Withania inhibited benzo(a)pyrene-induced forestomach papillomagenesis, showing up to 60 and 92% inhibition in tumor incidence and multiplicity, respectively. Similarly, Withania inhibited 7,12-dimethylbenzanthracene-induced skin papillomagenesis, showing up to 45 and 71% inhibition in tumor incidence and multiplicity. In both studies, Withania showed no apparent toxic effects in mice as monitored by the body weight gain profile. Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

No MeSH data available.


Related in: MedlinePlus