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Roots of Withania somnifera Inhibit Forestomach and Skin Carcinogenesis in Mice.

Padmavathi B, Rath PC, Rao AR, Singh RP - Evid Based Complement Alternat Med (2005)

Bottom Line: We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model.Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver.Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

View Article: PubMed Central - PubMed

ABSTRACT
We evaluated the cancer chemopreventive efficacy of the Withania somnifera root, which has been used in the Indian traditional medicine system for many centuries for the treatment of various ailments. Since, studies showing its mechanism-based cancer chemopreventive efficacy are limited, this was investigated in the present study. We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model. Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver. Further, in a long-term tumorigenesis study, Withania inhibited benzo(a)pyrene-induced forestomach papillomagenesis, showing up to 60 and 92% inhibition in tumor incidence and multiplicity, respectively. Similarly, Withania inhibited 7,12-dimethylbenzanthracene-induced skin papillomagenesis, showing up to 45 and 71% inhibition in tumor incidence and multiplicity. In both studies, Withania showed no apparent toxic effects in mice as monitored by the body weight gain profile. Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

No MeSH data available.


Related in: MedlinePlus

Effect of roots of Withania on DMBA-induced papillomas in mouse skin. Pictures were taken at the end of the experiment and are representative for each group. Treatments were as indicated in the figure.
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fig5: Effect of roots of Withania on DMBA-induced papillomas in mouse skin. Pictures were taken at the end of the experiment and are representative for each group. Treatments were as indicated in the figure.

Mentions: A representative picture of mice bearing skin papillomas in each group is shown in Fig. 5. A single topical application of 50 μg of DMBA/animal in acetone to the dorsal skin followed by croton oil treatment induced tumors in 80% of the control group of mice (12 out of 15) after 12 weeks of DMBA treatment (Fig. 6A). The mean number of papillomas in the control group was 3.17 ± 2.4 papillomas/animal (Fig. 6B). The experimental group fed with the lower dose (2.5%, w/w) Withania diet (2 weeks before and 2 weeks after the DMBA treatment) showed a 53% tumor incidence (Fig. 6A) and 1.1 ± 1.6 papillomas/animal at the end of the experiment (Fig. 6B). However, we observed no increase in tumor multiplicity after 9 weeks of DMBA treatment. Mice fed with the higher dose (5%, w/w) Withania diet showed only a 44% tumor incidence (Fig. 6A) and 0.93 ± 1.58 papillomas/animal (Fig. 6B). We also observed that the high dose of Withania diet delayed tumor appearance by 2 weeks (Fig. 6B). Further, skin tumors (Fig. 7B) were processed for histopathological evaluation by hematoxylin and eosin staining, in which tumors of the control group of mice showed a relatively progressive stage of skin tumor development as characterized by their cellular and nuclear morphology as well as dermal invasiveness. These results demonstrate the preventive efficacy of Withania root against carcinogen-induced skin tumorigenesis in mice.


Roots of Withania somnifera Inhibit Forestomach and Skin Carcinogenesis in Mice.

Padmavathi B, Rath PC, Rao AR, Singh RP - Evid Based Complement Alternat Med (2005)

Effect of roots of Withania on DMBA-induced papillomas in mouse skin. Pictures were taken at the end of the experiment and are representative for each group. Treatments were as indicated in the figure.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1062157&req=5

fig5: Effect of roots of Withania on DMBA-induced papillomas in mouse skin. Pictures were taken at the end of the experiment and are representative for each group. Treatments were as indicated in the figure.
Mentions: A representative picture of mice bearing skin papillomas in each group is shown in Fig. 5. A single topical application of 50 μg of DMBA/animal in acetone to the dorsal skin followed by croton oil treatment induced tumors in 80% of the control group of mice (12 out of 15) after 12 weeks of DMBA treatment (Fig. 6A). The mean number of papillomas in the control group was 3.17 ± 2.4 papillomas/animal (Fig. 6B). The experimental group fed with the lower dose (2.5%, w/w) Withania diet (2 weeks before and 2 weeks after the DMBA treatment) showed a 53% tumor incidence (Fig. 6A) and 1.1 ± 1.6 papillomas/animal at the end of the experiment (Fig. 6B). However, we observed no increase in tumor multiplicity after 9 weeks of DMBA treatment. Mice fed with the higher dose (5%, w/w) Withania diet showed only a 44% tumor incidence (Fig. 6A) and 0.93 ± 1.58 papillomas/animal (Fig. 6B). We also observed that the high dose of Withania diet delayed tumor appearance by 2 weeks (Fig. 6B). Further, skin tumors (Fig. 7B) were processed for histopathological evaluation by hematoxylin and eosin staining, in which tumors of the control group of mice showed a relatively progressive stage of skin tumor development as characterized by their cellular and nuclear morphology as well as dermal invasiveness. These results demonstrate the preventive efficacy of Withania root against carcinogen-induced skin tumorigenesis in mice.

Bottom Line: We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model.Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver.Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

View Article: PubMed Central - PubMed

ABSTRACT
We evaluated the cancer chemopreventive efficacy of the Withania somnifera root, which has been used in the Indian traditional medicine system for many centuries for the treatment of various ailments. Since, studies showing its mechanism-based cancer chemopreventive efficacy are limited, this was investigated in the present study. We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model. Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver. Further, in a long-term tumorigenesis study, Withania inhibited benzo(a)pyrene-induced forestomach papillomagenesis, showing up to 60 and 92% inhibition in tumor incidence and multiplicity, respectively. Similarly, Withania inhibited 7,12-dimethylbenzanthracene-induced skin papillomagenesis, showing up to 45 and 71% inhibition in tumor incidence and multiplicity. In both studies, Withania showed no apparent toxic effects in mice as monitored by the body weight gain profile. Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

No MeSH data available.


Related in: MedlinePlus