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Roots of Withania somnifera Inhibit Forestomach and Skin Carcinogenesis in Mice.

Padmavathi B, Rath PC, Rao AR, Singh RP - Evid Based Complement Alternat Med (2005)

Bottom Line: We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model.Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver.Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

View Article: PubMed Central - PubMed

ABSTRACT
We evaluated the cancer chemopreventive efficacy of the Withania somnifera root, which has been used in the Indian traditional medicine system for many centuries for the treatment of various ailments. Since, studies showing its mechanism-based cancer chemopreventive efficacy are limited, this was investigated in the present study. We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model. Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver. Further, in a long-term tumorigenesis study, Withania inhibited benzo(a)pyrene-induced forestomach papillomagenesis, showing up to 60 and 92% inhibition in tumor incidence and multiplicity, respectively. Similarly, Withania inhibited 7,12-dimethylbenzanthracene-induced skin papillomagenesis, showing up to 45 and 71% inhibition in tumor incidence and multiplicity. In both studies, Withania showed no apparent toxic effects in mice as monitored by the body weight gain profile. Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

No MeSH data available.


Related in: MedlinePlus

Roots of Withania somnifera.
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fig1: Roots of Withania somnifera.

Mentions: The dried roots of W.somnifera were purchased from an authorized dealer in New Delhi, India, and were authenticated by a competent botanist, Dr Raveesha, at the Department of Botany, Mysore University, Mysore, India, and a voucher specimen was preserved in our laboratory (Fig. 1). The major active compounds in roots are reported to be withanolides, glycosides and many different alkaloids. To date, up to 19 withanolide derivatives have been isolated from Withania roots (17). The roots were thoroughly washed with 95% ethanol and with sterile water, and air-dried and powdered. Next, 125 g (2.5%, w/w) and 250 g (5%, w/w) of the powdered root were mixed uniformly with 5 kg of standard laboratory diet each and made into pellets. Mice were subjected to control diet and these experimental diets during the experiment as mentioned. We observed that 2 weeks of dietary feeding of Withania at these doses did not show any loss in body weight or reduction in diet consumption in mice; therefore, the same test diets were used for the study of tumorigenesis.


Roots of Withania somnifera Inhibit Forestomach and Skin Carcinogenesis in Mice.

Padmavathi B, Rath PC, Rao AR, Singh RP - Evid Based Complement Alternat Med (2005)

Roots of Withania somnifera.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC1062157&req=5

fig1: Roots of Withania somnifera.
Mentions: The dried roots of W.somnifera were purchased from an authorized dealer in New Delhi, India, and were authenticated by a competent botanist, Dr Raveesha, at the Department of Botany, Mysore University, Mysore, India, and a voucher specimen was preserved in our laboratory (Fig. 1). The major active compounds in roots are reported to be withanolides, glycosides and many different alkaloids. To date, up to 19 withanolide derivatives have been isolated from Withania roots (17). The roots were thoroughly washed with 95% ethanol and with sterile water, and air-dried and powdered. Next, 125 g (2.5%, w/w) and 250 g (5%, w/w) of the powdered root were mixed uniformly with 5 kg of standard laboratory diet each and made into pellets. Mice were subjected to control diet and these experimental diets during the experiment as mentioned. We observed that 2 weeks of dietary feeding of Withania at these doses did not show any loss in body weight or reduction in diet consumption in mice; therefore, the same test diets were used for the study of tumorigenesis.

Bottom Line: We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model.Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver.Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

View Article: PubMed Central - PubMed

ABSTRACT
We evaluated the cancer chemopreventive efficacy of the Withania somnifera root, which has been used in the Indian traditional medicine system for many centuries for the treatment of various ailments. Since, studies showing its mechanism-based cancer chemopreventive efficacy are limited, this was investigated in the present study. We studied the effect of dietary administration of Withania root on hepatic phase I, phase II and antioxidant enzymes by estimation of its level/activity, as well as in attenuating carcinogen-induced forestomach and skin tumorigenesis in the Swiss albino mouse model. Our findings showed that roots of W.somnifera inhibit phase I, and activates phase II and antioxidant enzymes in the liver. Further, in a long-term tumorigenesis study, Withania inhibited benzo(a)pyrene-induced forestomach papillomagenesis, showing up to 60 and 92% inhibition in tumor incidence and multiplicity, respectively. Similarly, Withania inhibited 7,12-dimethylbenzanthracene-induced skin papillomagenesis, showing up to 45 and 71% inhibition in tumor incidence and multiplicity. In both studies, Withania showed no apparent toxic effects in mice as monitored by the body weight gain profile. Together, these findings suggest that W.somnifera root has chemopreventive efficacy against forestomach and skin carcinogenesis and warrants the identification and isolation of active compounds responsible for its anticancer effects, which may provide the lead for the development of antitumor agents.

No MeSH data available.


Related in: MedlinePlus