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Aminoadipate reductase gene: a new fungal-specific gene for comparative evolutionary analyses.

An KD, Nishida H, Miura Y, Yokota A - BMC Evol. Biol. (2002)

Bottom Line: In fungi, aminoadipate reductase converts 2-aminoadipate to 2-aminoadipate 6-semialdehyde.These trees are consistent with current ascomycete systematics and demonstrate the potential utility of the aminoadipete reductase gene for phylogenetic analyses of fungi.We believe that the comparison of aminoadipate reductase among species will be useful for molecular ecological and evolutionary studies of fungi, because this enzyme-encoding gene is a fungal-specific gene and generally appears to be single copy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. aa17122@mail.ecc.u-tokyo.ac.jp

ABSTRACT

Background: In fungi, aminoadipate reductase converts 2-aminoadipate to 2-aminoadipate 6-semialdehyde. However, other organisms have no homologue to the aminoadipate reductase gene and this pathway appears to be restricted to fungi. In this study, we designed degenerate primers for polymerase chain reaction (PCR) amplification of a large fragment of the aminoadipate reductase gene for divergent fungi.

Results: Using these primers, we amplified DNA fragments from the archiascomycetous yeast Saitoella complicata and the black-koji mold Aspergillus awamori. Based on an alignment of the deduced amino acid sequences, we constructed phylogenetic trees. These trees are consistent with current ascomycete systematics and demonstrate the potential utility of the aminoadipete reductase gene for phylogenetic analyses of fungi.

Conclusions: We believe that the comparison of aminoadipate reductase among species will be useful for molecular ecological and evolutionary studies of fungi, because this enzyme-encoding gene is a fungal-specific gene and generally appears to be single copy.

Show MeSH
Reduction of 2-aminoadipate by aminoadipte reductase in fungi.
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Figure 1: Reduction of 2-aminoadipate by aminoadipte reductase in fungi.

Mentions: In the lysine biosynthetic pathway of the ascomycete yeast Saccharomyces cerevisiae, a complex of LYS2 (1392 aa) and LYS5 (272 aa) serves as aminoadipate reductase, which converts 2-aminoadipate into 2-aminoadipate 6-semialdehyde via an adenosylated derivative (Fig. 1). Before this report, seven coding regions of the large subunit of the aminoadipate reductase were deposited in the international DNA/protein database [10-15]. The Saccharomyces cerevisiae lys2 gene is a single-copy gene. Comparisons of single-copy genes of large size can provide good phylogenetic resolution and offer advantages over multi-copy genes [16]. According to the seven ascomycetous aminoadipate reductases, fungal the coding regions of lys2 genes are more than 4,000 nucleotides-long.


Aminoadipate reductase gene: a new fungal-specific gene for comparative evolutionary analyses.

An KD, Nishida H, Miura Y, Yokota A - BMC Evol. Biol. (2002)

Reduction of 2-aminoadipate by aminoadipte reductase in fungi.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC103663&req=5

Figure 1: Reduction of 2-aminoadipate by aminoadipte reductase in fungi.
Mentions: In the lysine biosynthetic pathway of the ascomycete yeast Saccharomyces cerevisiae, a complex of LYS2 (1392 aa) and LYS5 (272 aa) serves as aminoadipate reductase, which converts 2-aminoadipate into 2-aminoadipate 6-semialdehyde via an adenosylated derivative (Fig. 1). Before this report, seven coding regions of the large subunit of the aminoadipate reductase were deposited in the international DNA/protein database [10-15]. The Saccharomyces cerevisiae lys2 gene is a single-copy gene. Comparisons of single-copy genes of large size can provide good phylogenetic resolution and offer advantages over multi-copy genes [16]. According to the seven ascomycetous aminoadipate reductases, fungal the coding regions of lys2 genes are more than 4,000 nucleotides-long.

Bottom Line: In fungi, aminoadipate reductase converts 2-aminoadipate to 2-aminoadipate 6-semialdehyde.These trees are consistent with current ascomycete systematics and demonstrate the potential utility of the aminoadipete reductase gene for phylogenetic analyses of fungi.We believe that the comparison of aminoadipate reductase among species will be useful for molecular ecological and evolutionary studies of fungi, because this enzyme-encoding gene is a fungal-specific gene and generally appears to be single copy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan. aa17122@mail.ecc.u-tokyo.ac.jp

ABSTRACT

Background: In fungi, aminoadipate reductase converts 2-aminoadipate to 2-aminoadipate 6-semialdehyde. However, other organisms have no homologue to the aminoadipate reductase gene and this pathway appears to be restricted to fungi. In this study, we designed degenerate primers for polymerase chain reaction (PCR) amplification of a large fragment of the aminoadipate reductase gene for divergent fungi.

Results: Using these primers, we amplified DNA fragments from the archiascomycetous yeast Saitoella complicata and the black-koji mold Aspergillus awamori. Based on an alignment of the deduced amino acid sequences, we constructed phylogenetic trees. These trees are consistent with current ascomycete systematics and demonstrate the potential utility of the aminoadipete reductase gene for phylogenetic analyses of fungi.

Conclusions: We believe that the comparison of aminoadipate reductase among species will be useful for molecular ecological and evolutionary studies of fungi, because this enzyme-encoding gene is a fungal-specific gene and generally appears to be single copy.

Show MeSH