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Genetically modified parthenocarpic eggplants: improved fruit productivity under both greenhouse and open field cultivation.

Acciarri N, Restaino F, Vitelli G, Perrone D, Zottini M, Pandolfini T, Spena A, Rotino G - BMC Biotechnol. (2002)

Bottom Line: The iaaM gene codes for tryptophan monoxygenase and confers auxin synthesis, while the DefH9 controlling regions drive expression of the gene specifically in the ovules and placenta.The main advantages of DefH9-iaaM eggplants are: i) improved fruit productivity (at least 30-35%) under both greenhouse and open field cultivation; ii) production of good quality (marketable) fruits during different types of cultivation; iii) seedless fruit with improved quality.Such advantages have been achieved without the use of either male or female sterility genes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Institute for Vegetable Crops, Monsampolo del Tronto, AP, Italy. acciarri@libero.it

ABSTRACT

Background: Parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the DefH9-iaaM gene. The iaaM gene codes for tryptophan monoxygenase and confers auxin synthesis, while the DefH9 controlling regions drive expression of the gene specifically in the ovules and placenta. A previous greenhouse trial for winter production of genetically engineered (GM) parthenocarpic eggplants demonstrated a significant increase (an average of 33% increase) in fruit production concomitant with a reduction in cultivation costs.

Results: GM parthenocarpic eggplants have been evaluated in three field trials. Two greenhouse spring trials have shown that these plants outyielded the corresponding untransformed genotypes, while a summer trial has shown that improved fruit productivity in GM eggplants can also be achieved in open field cultivation. Since the fruits were always seedless, the quality of GM eggplant fruits was improved as well. RT-PCR analysis demonstrated that the DefH9-iaaM gene is expressed during late stages of fruit development.

Conclusions: The DefH9-iaaM parthenocarpic gene is a biotechnological tool that enhances the agronomic value of all eggplant genotypes tested. The main advantages of DefH9-iaaM eggplants are: i) improved fruit productivity (at least 30-35%) under both greenhouse and open field cultivation; ii) production of good quality (marketable) fruits during different types of cultivation; iii) seedless fruit with improved quality. Such advantages have been achieved without the use of either male or female sterility genes.

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Eggplant fruits from the open field summer trial. Left, uncut and cut fruit of the transgenic hybrid P10; Right, cut and uncut fruit of the C10 control hybrid.
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Figure 2: Eggplant fruits from the open field summer trial. Left, uncut and cut fruit of the transgenic hybrid P10; Right, cut and uncut fruit of the C10 control hybrid.

Mentions: Consisting of ten harvests, the total production of P1 hybrids was 37% higher than control C1 eggplants (Table 2). The difference in total yield between P1 and control C1 hybrids was statistically significant and due both to the higher number of fruits/plant and to the increased weight of GM fruits. It is noteworthy to point out that when considered individually, neither trait (number of fruits/plant or fruit weight) showed statistically significant differences between the GM and untransformed plants (Table 2). Although higher in P10 hybrids in comparison to its control C10, the total yield (the number and average weight of the fruits) was not significantly different between the two. During the whole harvesting period, fruits from both P1 and P10 parthenocarpic hybrids were always seedless (Fig. 2), whilst control fruits always had seeds. Therefore, under open field cultivation, the DefH9-iaaM gene had a positive influence on fruit quality, as GM DefH9-iaaM fruits were always seedless. Fruit quality affects the economic value of eggplant production.


Genetically modified parthenocarpic eggplants: improved fruit productivity under both greenhouse and open field cultivation.

Acciarri N, Restaino F, Vitelli G, Perrone D, Zottini M, Pandolfini T, Spena A, Rotino G - BMC Biotechnol. (2002)

Eggplant fruits from the open field summer trial. Left, uncut and cut fruit of the transgenic hybrid P10; Right, cut and uncut fruit of the C10 control hybrid.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC101493&req=5

Figure 2: Eggplant fruits from the open field summer trial. Left, uncut and cut fruit of the transgenic hybrid P10; Right, cut and uncut fruit of the C10 control hybrid.
Mentions: Consisting of ten harvests, the total production of P1 hybrids was 37% higher than control C1 eggplants (Table 2). The difference in total yield between P1 and control C1 hybrids was statistically significant and due both to the higher number of fruits/plant and to the increased weight of GM fruits. It is noteworthy to point out that when considered individually, neither trait (number of fruits/plant or fruit weight) showed statistically significant differences between the GM and untransformed plants (Table 2). Although higher in P10 hybrids in comparison to its control C10, the total yield (the number and average weight of the fruits) was not significantly different between the two. During the whole harvesting period, fruits from both P1 and P10 parthenocarpic hybrids were always seedless (Fig. 2), whilst control fruits always had seeds. Therefore, under open field cultivation, the DefH9-iaaM gene had a positive influence on fruit quality, as GM DefH9-iaaM fruits were always seedless. Fruit quality affects the economic value of eggplant production.

Bottom Line: The iaaM gene codes for tryptophan monoxygenase and confers auxin synthesis, while the DefH9 controlling regions drive expression of the gene specifically in the ovules and placenta.The main advantages of DefH9-iaaM eggplants are: i) improved fruit productivity (at least 30-35%) under both greenhouse and open field cultivation; ii) production of good quality (marketable) fruits during different types of cultivation; iii) seedless fruit with improved quality.Such advantages have been achieved without the use of either male or female sterility genes.

View Article: PubMed Central - HTML - PubMed

Affiliation: Research Institute for Vegetable Crops, Monsampolo del Tronto, AP, Italy. acciarri@libero.it

ABSTRACT

Background: Parthenocarpy, or fruit development in the absence of fertilization, has been genetically engineered in eggplant and in other horticultural species by using the DefH9-iaaM gene. The iaaM gene codes for tryptophan monoxygenase and confers auxin synthesis, while the DefH9 controlling regions drive expression of the gene specifically in the ovules and placenta. A previous greenhouse trial for winter production of genetically engineered (GM) parthenocarpic eggplants demonstrated a significant increase (an average of 33% increase) in fruit production concomitant with a reduction in cultivation costs.

Results: GM parthenocarpic eggplants have been evaluated in three field trials. Two greenhouse spring trials have shown that these plants outyielded the corresponding untransformed genotypes, while a summer trial has shown that improved fruit productivity in GM eggplants can also be achieved in open field cultivation. Since the fruits were always seedless, the quality of GM eggplant fruits was improved as well. RT-PCR analysis demonstrated that the DefH9-iaaM gene is expressed during late stages of fruit development.

Conclusions: The DefH9-iaaM parthenocarpic gene is a biotechnological tool that enhances the agronomic value of all eggplant genotypes tested. The main advantages of DefH9-iaaM eggplants are: i) improved fruit productivity (at least 30-35%) under both greenhouse and open field cultivation; ii) production of good quality (marketable) fruits during different types of cultivation; iii) seedless fruit with improved quality. Such advantages have been achieved without the use of either male or female sterility genes.

Show MeSH
Related in: MedlinePlus