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Phylogenetic relationships between Hapalemur species and subspecies based on mitochondrial DNA sequences.

Fausser JL, Prosper P, Donati G, Ramanamanjato JB, Rumpler Y - BMC Evol. Biol. (2002)

Bottom Line: A new chromosomal polymorphic variant from the region of Ranomafana, H. griseus ssp, has been analysed and was found in both clades.Our results support the raising of H. g. meridionalis to the specific rank H. meridionalis, while neither cytogenetic nor molecular evidences support the raising of H. g. alaotrensis to a species rank despite its morphological characteristics.This suggests the existence of an ancestral polymorphism or an introgression of mitochondrial DNA between subspecies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut d'Embryologie, EA3428, 67085 Strasbourg, France. marguerite.lavaux@embryo-ulp.u-strasbg.fr

ABSTRACT

Background: Phylogenetic relationships of the genus Hapalemur remains controversial, particularly within the Hapalemur griseus species group. In order to obtain more information on the taxonomic status within this genus, and particularly in the cytogenetic distinct subspecies group of Hapalemur griseus, 357 bp sequence of cytochrome b and 438 bp of 12S mitochondrial DNAs were analyzed on a sample of animals captured in areas extending from the north to the south-east of Madagascar. This sample covers all cytogenetically defined types recognized of the genus Hapalemur.

Results: Phylogenetic trees and distances analyses demonstrate a first emergence of Hapalemur simus followed by H. aureus which is the sister clade of the H. griseus subspecies. Hapalemur griseus is composed of 4 subspecies separated into two clades. The first contains H. g. griseus, H. g. alaotrensis and H. g. occidentalis. The second consists of H. g. meridionalis. A new chromosomal polymorphic variant from the region of Ranomafana, H. griseus ssp, has been analysed and was found in both clades.

Conclusions: Our results support the raising of H. g. meridionalis to the specific rank H. meridionalis, while neither cytogenetic nor molecular evidences support the raising of H. g. alaotrensis to a species rank despite its morphological characteristics. The new cytotype H. g. ssp which has been previously characterized by cytogenetic studies contains animals clustering either with the group of Hapalemur griseus griseus or with that of Hapalemur meridionalis. This suggests the existence of an ancestral polymorphism or an introgression of mitochondrial DNA between subspecies.

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Map of Madagascar showing the capture locations and/or the origin of the different Hapalemur species and subspecies, a) Analamera, b) Ambato, c) Ambakoany, d) Maroantsetra, e) Alaotra lake, f) Maromiza, g) Tsimbazaza zoo, h) Ranomafana, i) AmbolomavoJ) Kianjavato, k) Andohahela, l) Mandena. Abbreviations : HSI = Hapalemur simus, HAU = H. aureus, HGM = H. griseus meridionalis, HGsspb= H. griseus sspb, HGsspa= H. griseus sspa, HGG = H. g. griseus, HGA = H. g. alaotrensis, HGO = H. g. occidentalis. The first number behind each taxon represents the number of animals captured and the second, the number of haplotype found in this area. *The haplotype HGsspa02 is present in h, i and j; Hgsspa03 in h and j; Hgsspa07 in h and i; HGsspa10 in h and i.
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Figure 1: Map of Madagascar showing the capture locations and/or the origin of the different Hapalemur species and subspecies, a) Analamera, b) Ambato, c) Ambakoany, d) Maroantsetra, e) Alaotra lake, f) Maromiza, g) Tsimbazaza zoo, h) Ranomafana, i) AmbolomavoJ) Kianjavato, k) Andohahela, l) Mandena. Abbreviations : HSI = Hapalemur simus, HAU = H. aureus, HGM = H. griseus meridionalis, HGsspb= H. griseus sspb, HGsspa= H. griseus sspa, HGG = H. g. griseus, HGA = H. g. alaotrensis, HGO = H. g. occidentalis. The first number behind each taxon represents the number of animals captured and the second, the number of haplotype found in this area. *The haplotype HGsspa02 is present in h, i and j; Hgsspa03 in h and j; Hgsspa07 in h and i; HGsspa10 in h and i.

Mentions: A total of 115 animals covering all the genus Hapalemur were captured in bamboo forests extending from the north to the south of Madagascar. The different capture areas of each species and subspecies as the number of animals are indicated in Fig. 1.


Phylogenetic relationships between Hapalemur species and subspecies based on mitochondrial DNA sequences.

Fausser JL, Prosper P, Donati G, Ramanamanjato JB, Rumpler Y - BMC Evol. Biol. (2002)

Map of Madagascar showing the capture locations and/or the origin of the different Hapalemur species and subspecies, a) Analamera, b) Ambato, c) Ambakoany, d) Maroantsetra, e) Alaotra lake, f) Maromiza, g) Tsimbazaza zoo, h) Ranomafana, i) AmbolomavoJ) Kianjavato, k) Andohahela, l) Mandena. Abbreviations : HSI = Hapalemur simus, HAU = H. aureus, HGM = H. griseus meridionalis, HGsspb= H. griseus sspb, HGsspa= H. griseus sspa, HGG = H. g. griseus, HGA = H. g. alaotrensis, HGO = H. g. occidentalis. The first number behind each taxon represents the number of animals captured and the second, the number of haplotype found in this area. *The haplotype HGsspa02 is present in h, i and j; Hgsspa03 in h and j; Hgsspa07 in h and i; HGsspa10 in h and i.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC101410&req=5

Figure 1: Map of Madagascar showing the capture locations and/or the origin of the different Hapalemur species and subspecies, a) Analamera, b) Ambato, c) Ambakoany, d) Maroantsetra, e) Alaotra lake, f) Maromiza, g) Tsimbazaza zoo, h) Ranomafana, i) AmbolomavoJ) Kianjavato, k) Andohahela, l) Mandena. Abbreviations : HSI = Hapalemur simus, HAU = H. aureus, HGM = H. griseus meridionalis, HGsspb= H. griseus sspb, HGsspa= H. griseus sspa, HGG = H. g. griseus, HGA = H. g. alaotrensis, HGO = H. g. occidentalis. The first number behind each taxon represents the number of animals captured and the second, the number of haplotype found in this area. *The haplotype HGsspa02 is present in h, i and j; Hgsspa03 in h and j; Hgsspa07 in h and i; HGsspa10 in h and i.
Mentions: A total of 115 animals covering all the genus Hapalemur were captured in bamboo forests extending from the north to the south of Madagascar. The different capture areas of each species and subspecies as the number of animals are indicated in Fig. 1.

Bottom Line: A new chromosomal polymorphic variant from the region of Ranomafana, H. griseus ssp, has been analysed and was found in both clades.Our results support the raising of H. g. meridionalis to the specific rank H. meridionalis, while neither cytogenetic nor molecular evidences support the raising of H. g. alaotrensis to a species rank despite its morphological characteristics.This suggests the existence of an ancestral polymorphism or an introgression of mitochondrial DNA between subspecies.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institut d'Embryologie, EA3428, 67085 Strasbourg, France. marguerite.lavaux@embryo-ulp.u-strasbg.fr

ABSTRACT

Background: Phylogenetic relationships of the genus Hapalemur remains controversial, particularly within the Hapalemur griseus species group. In order to obtain more information on the taxonomic status within this genus, and particularly in the cytogenetic distinct subspecies group of Hapalemur griseus, 357 bp sequence of cytochrome b and 438 bp of 12S mitochondrial DNAs were analyzed on a sample of animals captured in areas extending from the north to the south-east of Madagascar. This sample covers all cytogenetically defined types recognized of the genus Hapalemur.

Results: Phylogenetic trees and distances analyses demonstrate a first emergence of Hapalemur simus followed by H. aureus which is the sister clade of the H. griseus subspecies. Hapalemur griseus is composed of 4 subspecies separated into two clades. The first contains H. g. griseus, H. g. alaotrensis and H. g. occidentalis. The second consists of H. g. meridionalis. A new chromosomal polymorphic variant from the region of Ranomafana, H. griseus ssp, has been analysed and was found in both clades.

Conclusions: Our results support the raising of H. g. meridionalis to the specific rank H. meridionalis, while neither cytogenetic nor molecular evidences support the raising of H. g. alaotrensis to a species rank despite its morphological characteristics. The new cytotype H. g. ssp which has been previously characterized by cytogenetic studies contains animals clustering either with the group of Hapalemur griseus griseus or with that of Hapalemur meridionalis. This suggests the existence of an ancestral polymorphism or an introgression of mitochondrial DNA between subspecies.

Show MeSH