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Elevated serum immunoglobulin E to Cryptomeria japonica pollen in rats exposed to diesel exhaust during fetal and neonatal periods.

Watanabe N, Ohsawa M - BMC Pregnancy Childbirth (2002)

Bottom Line: There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively).IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each).CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Environmental Health, Tokyo Metropolitan Research Laboratory of Public Health, Tokyo, Japan. nobuew@toky-eiken.go.jp

ABSTRACT
BACKGROUND: The possible participation of diesel exhaust inhalation during the fetal period in the elevation of IgE against pollen in postnatal life was investigated. METHOD: The experiment was conducted using rat pups. Group I; exposed to clean air (Control). Group 2, 3; exposed to total or filtered diesel exhaust during the fetal period (Total-C-C, Filtered-C-C). Group 4, 5; exposed to total or filtered diesel exhaust during the suckling period (C-Total-C, C-Filtered-C). Group 6,7; exposed to total or filtered diesel exhaust during the weaning period (C-C-Total, C-C-Filtered). Total diesel engine exhaust contained 1.73 mg/m3 particulate matter and 0.79 ppm nitrogen dioxide; filtered exhaust contained the same gases as the total exhaust without particulate matter. Intraperitoneal injection of 5 mg crude cedar pollen was performed at 2-week intervals from the 49th day after birth. RESULTS: The mean IgE titers measured by the P-K reaction in the Control, Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered were 64.0 ± 2.7, 469.5 ± 1.6, 332.0 ± 1.7, 380.4 ± 1.7, 394.8 ± 1.7, 115.9 ± 1.3 and 57.0 ± 2.8 respectively after the fourth immunization. There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively). The mean IgE titers by ELISA were 0.47 ± 0.06, 0.79 ± 0.35, 0.86 ± 0.46, 0.80 ± 0.22, 0.56 ± 0.08, 0.46 ± 0.04 and 0.45 ± 0.03, respectively. IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each). CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

No MeSH data available.


Related in: MedlinePlus

Anti-Japanese cedar pollen IgE titers measured by P-K reaction. • IgE titers in the individual rat; * geometrical means of each group
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Figure 2: Anti-Japanese cedar pollen IgE titers measured by P-K reaction. • IgE titers in the individual rat; * geometrical means of each group

Mentions: The individual anti-Japanese cedar pollen IgE antibody titer measured by the Prausnitz-Kustner reaction (P-K reaction) and the mean IgE titers in Control, Total-C-C, Filtered-C-C. C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered after the third and fourth immunization are shown in Figure 2. The geometrical means of IgE titers in Control, Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered were 32.0 ± 6.9, 181.0 ± 1.5, 166.0 ± 1.4, 49.4 ± 4.0, 43.1 ± 6.5, 4.0 ± 1.0 and 6.3 ± 2.0 after the third immunization, and 64.0 ± 2.7, 469.5 ± 1.6, 332.0 ± 1.7, 380.4 ± 1.7, 394.8 ± 1.7, 115.9 ± 1.3 and 57.0 ± 2.8 after the fourth immunization, respectively. Log2 P-K IgE titers of each group are shown in Table 3. There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control after the fourth immunization (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively). There were no differences between the total-exposure and filtered-exposure conditions.


Elevated serum immunoglobulin E to Cryptomeria japonica pollen in rats exposed to diesel exhaust during fetal and neonatal periods.

Watanabe N, Ohsawa M - BMC Pregnancy Childbirth (2002)

Anti-Japanese cedar pollen IgE titers measured by P-K reaction. • IgE titers in the individual rat; * geometrical means of each group
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC101391&req=5

Figure 2: Anti-Japanese cedar pollen IgE titers measured by P-K reaction. • IgE titers in the individual rat; * geometrical means of each group
Mentions: The individual anti-Japanese cedar pollen IgE antibody titer measured by the Prausnitz-Kustner reaction (P-K reaction) and the mean IgE titers in Control, Total-C-C, Filtered-C-C. C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered after the third and fourth immunization are shown in Figure 2. The geometrical means of IgE titers in Control, Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered were 32.0 ± 6.9, 181.0 ± 1.5, 166.0 ± 1.4, 49.4 ± 4.0, 43.1 ± 6.5, 4.0 ± 1.0 and 6.3 ± 2.0 after the third immunization, and 64.0 ± 2.7, 469.5 ± 1.6, 332.0 ± 1.7, 380.4 ± 1.7, 394.8 ± 1.7, 115.9 ± 1.3 and 57.0 ± 2.8 after the fourth immunization, respectively. Log2 P-K IgE titers of each group are shown in Table 3. There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control after the fourth immunization (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively). There were no differences between the total-exposure and filtered-exposure conditions.

Bottom Line: There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively).IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each).CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Environmental Health, Tokyo Metropolitan Research Laboratory of Public Health, Tokyo, Japan. nobuew@toky-eiken.go.jp

ABSTRACT
BACKGROUND: The possible participation of diesel exhaust inhalation during the fetal period in the elevation of IgE against pollen in postnatal life was investigated. METHOD: The experiment was conducted using rat pups. Group I; exposed to clean air (Control). Group 2, 3; exposed to total or filtered diesel exhaust during the fetal period (Total-C-C, Filtered-C-C). Group 4, 5; exposed to total or filtered diesel exhaust during the suckling period (C-Total-C, C-Filtered-C). Group 6,7; exposed to total or filtered diesel exhaust during the weaning period (C-C-Total, C-C-Filtered). Total diesel engine exhaust contained 1.73 mg/m3 particulate matter and 0.79 ppm nitrogen dioxide; filtered exhaust contained the same gases as the total exhaust without particulate matter. Intraperitoneal injection of 5 mg crude cedar pollen was performed at 2-week intervals from the 49th day after birth. RESULTS: The mean IgE titers measured by the P-K reaction in the Control, Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered were 64.0 ± 2.7, 469.5 ± 1.6, 332.0 ± 1.7, 380.4 ± 1.7, 394.8 ± 1.7, 115.9 ± 1.3 and 57.0 ± 2.8 respectively after the fourth immunization. There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively). The mean IgE titers by ELISA were 0.47 ± 0.06, 0.79 ± 0.35, 0.86 ± 0.46, 0.80 ± 0.22, 0.56 ± 0.08, 0.46 ± 0.04 and 0.45 ± 0.03, respectively. IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each). CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

No MeSH data available.


Related in: MedlinePlus