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Elevated serum immunoglobulin E to Cryptomeria japonica pollen in rats exposed to diesel exhaust during fetal and neonatal periods.

Watanabe N, Ohsawa M - BMC Pregnancy Childbirth (2002)

Bottom Line: There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively).IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each).CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Environmental Health, Tokyo Metropolitan Research Laboratory of Public Health, Tokyo, Japan. nobuew@toky-eiken.go.jp

ABSTRACT
BACKGROUND: The possible participation of diesel exhaust inhalation during the fetal period in the elevation of IgE against pollen in postnatal life was investigated. METHOD: The experiment was conducted using rat pups. Group I; exposed to clean air (Control). Group 2, 3; exposed to total or filtered diesel exhaust during the fetal period (Total-C-C, Filtered-C-C). Group 4, 5; exposed to total or filtered diesel exhaust during the suckling period (C-Total-C, C-Filtered-C). Group 6,7; exposed to total or filtered diesel exhaust during the weaning period (C-C-Total, C-C-Filtered). Total diesel engine exhaust contained 1.73 mg/m3 particulate matter and 0.79 ppm nitrogen dioxide; filtered exhaust contained the same gases as the total exhaust without particulate matter. Intraperitoneal injection of 5 mg crude cedar pollen was performed at 2-week intervals from the 49th day after birth. RESULTS: The mean IgE titers measured by the P-K reaction in the Control, Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered were 64.0 ± 2.7, 469.5 ± 1.6, 332.0 ± 1.7, 380.4 ± 1.7, 394.8 ± 1.7, 115.9 ± 1.3 and 57.0 ± 2.8 respectively after the fourth immunization. There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively). The mean IgE titers by ELISA were 0.47 ± 0.06, 0.79 ± 0.35, 0.86 ± 0.46, 0.80 ± 0.22, 0.56 ± 0.08, 0.46 ± 0.04 and 0.45 ± 0.03, respectively. IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each). CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

No MeSH data available.


Related in: MedlinePlus

Experimental protocol. Control, exposed to clean air; Total-C-C, exposed to total diesel exhaust from the 7th day of gestation until 3 days after birth; Filtered-C-C, exposed to filtered exhaust from the 7th days of gestation until 3 days after birth; C-Total-C, exposed to diesel exhaust from the 4th day after birth through the 22nd day after birth; C-Filtered-C, exposed to filtered exhaust from the 4th day after birth through the 22nd day after birth; C-C-Total, exposed to diesel exhaust from day 23 after birth to day 41; C-C-Filtered, exposed to filtered exhaust from day 23 after birth to day 41.
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Figure 1: Experimental protocol. Control, exposed to clean air; Total-C-C, exposed to total diesel exhaust from the 7th day of gestation until 3 days after birth; Filtered-C-C, exposed to filtered exhaust from the 7th days of gestation until 3 days after birth; C-Total-C, exposed to diesel exhaust from the 4th day after birth through the 22nd day after birth; C-Filtered-C, exposed to filtered exhaust from the 4th day after birth through the 22nd day after birth; C-C-Total, exposed to diesel exhaust from day 23 after birth to day 41; C-C-Filtered, exposed to filtered exhaust from day 23 after birth to day 41.

Mentions: Forty-three pregnant Fischer rats (F344/DuCrj) obtained from Charles River Japan (Kanagawa, Japan) were divided into seven groups on the sixth days of pregnancy. The experiment was conducted using only male pups from those mothers. Group 1 was exposed to clean air during all of the experimental period (Control). Group 2 was exposed to total diesel exhaust from the 7th day of gestation (day of impregnation = day 0) until 3 days after birth, then exposed to clean air until days 49, 82 and 96 after birth. (Total-C-C). Group 3 was exposed to filtered exhaust from the 7th day of gestation until 3 days after birth, then exposed to clean air until days 49, 82 and 96 after birth (Filtered-C-C). Group 4 was exposed to diesel exhaust from the 4th day after birth through the 22nd day after birth. The group was exposed to clean air during the other periods of the experiment (C-Total-C). Group 5 was exposed to filtered exhaust from the 4th day after birth through the 22nd day after birth. The group was exposed to clean air during the other periods of the experiment (C-Filtered-C). Group 6 was exposed to diesel exhaust from day 23 after birth until day 41. The group was exposed to clean air during the other periods of the experiment (C-C-Total). Group 7 was exposed to filtered exhaust from day 23 after birth until day 41. The group was exposed to clean air during the other periods of the experiment (C-C-Filtered). Each group had the same exposure period, 19 days, to diesel exhaust. The number of pregnant rats for groups 1, 2, 3, 4, 5, 6 and 7 were 7, 7, 7, 6, 6, 5 and 5, respectively. Litter sizes ranged from 6 to 11. The total number of pups (male: female) of each group from the mothers were 53 (28:25), 50 (24:26), 54 (25:29), 47 (22:25), 44 (23:21), 43 (22:21) and 45 (20:25), respectively. The experimental protocol is summarized in Figure 1.


Elevated serum immunoglobulin E to Cryptomeria japonica pollen in rats exposed to diesel exhaust during fetal and neonatal periods.

Watanabe N, Ohsawa M - BMC Pregnancy Childbirth (2002)

Experimental protocol. Control, exposed to clean air; Total-C-C, exposed to total diesel exhaust from the 7th day of gestation until 3 days after birth; Filtered-C-C, exposed to filtered exhaust from the 7th days of gestation until 3 days after birth; C-Total-C, exposed to diesel exhaust from the 4th day after birth through the 22nd day after birth; C-Filtered-C, exposed to filtered exhaust from the 4th day after birth through the 22nd day after birth; C-C-Total, exposed to diesel exhaust from day 23 after birth to day 41; C-C-Filtered, exposed to filtered exhaust from day 23 after birth to day 41.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
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Figure 1: Experimental protocol. Control, exposed to clean air; Total-C-C, exposed to total diesel exhaust from the 7th day of gestation until 3 days after birth; Filtered-C-C, exposed to filtered exhaust from the 7th days of gestation until 3 days after birth; C-Total-C, exposed to diesel exhaust from the 4th day after birth through the 22nd day after birth; C-Filtered-C, exposed to filtered exhaust from the 4th day after birth through the 22nd day after birth; C-C-Total, exposed to diesel exhaust from day 23 after birth to day 41; C-C-Filtered, exposed to filtered exhaust from day 23 after birth to day 41.
Mentions: Forty-three pregnant Fischer rats (F344/DuCrj) obtained from Charles River Japan (Kanagawa, Japan) were divided into seven groups on the sixth days of pregnancy. The experiment was conducted using only male pups from those mothers. Group 1 was exposed to clean air during all of the experimental period (Control). Group 2 was exposed to total diesel exhaust from the 7th day of gestation (day of impregnation = day 0) until 3 days after birth, then exposed to clean air until days 49, 82 and 96 after birth. (Total-C-C). Group 3 was exposed to filtered exhaust from the 7th day of gestation until 3 days after birth, then exposed to clean air until days 49, 82 and 96 after birth (Filtered-C-C). Group 4 was exposed to diesel exhaust from the 4th day after birth through the 22nd day after birth. The group was exposed to clean air during the other periods of the experiment (C-Total-C). Group 5 was exposed to filtered exhaust from the 4th day after birth through the 22nd day after birth. The group was exposed to clean air during the other periods of the experiment (C-Filtered-C). Group 6 was exposed to diesel exhaust from day 23 after birth until day 41. The group was exposed to clean air during the other periods of the experiment (C-C-Total). Group 7 was exposed to filtered exhaust from day 23 after birth until day 41. The group was exposed to clean air during the other periods of the experiment (C-C-Filtered). Each group had the same exposure period, 19 days, to diesel exhaust. The number of pregnant rats for groups 1, 2, 3, 4, 5, 6 and 7 were 7, 7, 7, 6, 6, 5 and 5, respectively. Litter sizes ranged from 6 to 11. The total number of pups (male: female) of each group from the mothers were 53 (28:25), 50 (24:26), 54 (25:29), 47 (22:25), 44 (23:21), 43 (22:21) and 45 (20:25), respectively. The experimental protocol is summarized in Figure 1.

Bottom Line: There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively).IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each).CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Environmental Health, Tokyo Metropolitan Research Laboratory of Public Health, Tokyo, Japan. nobuew@toky-eiken.go.jp

ABSTRACT
BACKGROUND: The possible participation of diesel exhaust inhalation during the fetal period in the elevation of IgE against pollen in postnatal life was investigated. METHOD: The experiment was conducted using rat pups. Group I; exposed to clean air (Control). Group 2, 3; exposed to total or filtered diesel exhaust during the fetal period (Total-C-C, Filtered-C-C). Group 4, 5; exposed to total or filtered diesel exhaust during the suckling period (C-Total-C, C-Filtered-C). Group 6,7; exposed to total or filtered diesel exhaust during the weaning period (C-C-Total, C-C-Filtered). Total diesel engine exhaust contained 1.73 mg/m3 particulate matter and 0.79 ppm nitrogen dioxide; filtered exhaust contained the same gases as the total exhaust without particulate matter. Intraperitoneal injection of 5 mg crude cedar pollen was performed at 2-week intervals from the 49th day after birth. RESULTS: The mean IgE titers measured by the P-K reaction in the Control, Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C, C-C-Total and C-C-Filtered were 64.0 ± 2.7, 469.5 ± 1.6, 332.0 ± 1.7, 380.4 ± 1.7, 394.8 ± 1.7, 115.9 ± 1.3 and 57.0 ± 2.8 respectively after the fourth immunization. There were significant differences between Total-C-C, Filtered-C-C, C-Total-C, C-Filtered-C and Control (p < 0.01, p < 0.01, p < 0.05, p < 0.01, respectively). The mean IgE titers by ELISA were 0.47 ± 0.06, 0.79 ± 0.35, 0.86 ± 0.46, 0.80 ± 0.22, 0.56 ± 0.08, 0.46 ± 0.04 and 0.45 ± 0.03, respectively. IgE titers in Filtered-C-C and C-Total-C were significantly higher than in Control (p < 0.05 for each). CONCLUSIONS: Inhalation of diesel exhaust during differentiation of the immune system accelerated the elevation of IgE against pollen.

No MeSH data available.


Related in: MedlinePlus