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Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice.

Hosseinzadeh H, Younesi HM - BMC Pharmacol. (2002)

Bottom Line: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent.The extracts exhibited antinociceptive activity against acetic acid induced writhing.We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran. hosseinzadehh@yahoo.com

ABSTRACT

Background: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent. We aimed to evaluate the antinociceptive and anti-inflammatory activity of saffron extracts in mice.

Results: We used aqueous and ethanolic maceration extracts of Crocus sativus L. stigma and petals. Antinociceptive activity was examined using the hot plate and writhing tests. The effect of extracts against acute inflammation was studied using xylene induced ear edema in mice. The activity of the extracts against chronic inflammation was assessed by formalin-induced edema in the rat paw. In the hot plate tests, intraperitoneal injection of both extracts showed no significant antinociceptive activity in mice. The extracts exhibited antinociceptive activity against acetic acid induced writhing. Naloxone partially blocked only the antinociceptive activity of the stigma aqueous extract. Only the stigma extracts showed weak to moderate effect against acute inflammation. In chronic inflammation, both aqueous and ethanolic stigma extracts, as well as ethanolic petal extract, exerted anti-inflammatory effects.

Conclusions: We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

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Effect of the ethanolic extract of Crocus sativus petal and morphine (i.p.) on pain threshold of mice in the hot-plate test. Each point represents the mean ± S.E.M. of reaction time for n = 8 experiments on mice. ***P<0.001, compared to control (normal saline), Tukey-Kramer test.
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Figure 6: Effect of the ethanolic extract of Crocus sativus petal and morphine (i.p.) on pain threshold of mice in the hot-plate test. Each point represents the mean ± S.E.M. of reaction time for n = 8 experiments on mice. ***P<0.001, compared to control (normal saline), Tukey-Kramer test.

Mentions: In hot plate test, aqueous (1.4, 2.5 and 3.6 g/kg, i.p.) and ethanolic (0.8, 3.2 and 5.6 g/kg, i.p.) petal extracts showed no significant antinociceptive activity (P>0.05)(figures 5 and 6). The aqueous (0.32, 0.56 and 0.8 g/kg, i.p.) and ethanolic (0.8, 1.4 and 2 g/kg, i.p.) stigma extracts also exerted no significant analgesic activity (P>0.05)(figures 7 and 8). Morphine, a positive reference, (10 mg/kg, i.p.) showed significant analgesic effect in the hot-plate test beginning 30 min after treatment (P<0.001). In the xylene induced ear edema, the aqueous and ethanolic extracts of petal showed no significant anti-inflammatory activity but diclofenac and dexamethasone reduced the edema about 50% (Tables 2 and 3). In higher doses, the aqueous (0.56 g/kg, P<0.05; 0.8 g/kg, P<0.01) and ethanolic (1.4 g/kg, P<0.05; 2 g/kg, P<0.05) extracts of stigma showed significant activity against the acute inflammation (Tables 4 and 5).


Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice.

Hosseinzadeh H, Younesi HM - BMC Pharmacol. (2002)

Effect of the ethanolic extract of Crocus sativus petal and morphine (i.p.) on pain threshold of mice in the hot-plate test. Each point represents the mean ± S.E.M. of reaction time for n = 8 experiments on mice. ***P<0.001, compared to control (normal saline), Tukey-Kramer test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC101384&req=5

Figure 6: Effect of the ethanolic extract of Crocus sativus petal and morphine (i.p.) on pain threshold of mice in the hot-plate test. Each point represents the mean ± S.E.M. of reaction time for n = 8 experiments on mice. ***P<0.001, compared to control (normal saline), Tukey-Kramer test.
Mentions: In hot plate test, aqueous (1.4, 2.5 and 3.6 g/kg, i.p.) and ethanolic (0.8, 3.2 and 5.6 g/kg, i.p.) petal extracts showed no significant antinociceptive activity (P>0.05)(figures 5 and 6). The aqueous (0.32, 0.56 and 0.8 g/kg, i.p.) and ethanolic (0.8, 1.4 and 2 g/kg, i.p.) stigma extracts also exerted no significant analgesic activity (P>0.05)(figures 7 and 8). Morphine, a positive reference, (10 mg/kg, i.p.) showed significant analgesic effect in the hot-plate test beginning 30 min after treatment (P<0.001). In the xylene induced ear edema, the aqueous and ethanolic extracts of petal showed no significant anti-inflammatory activity but diclofenac and dexamethasone reduced the edema about 50% (Tables 2 and 3). In higher doses, the aqueous (0.56 g/kg, P<0.05; 0.8 g/kg, P<0.01) and ethanolic (1.4 g/kg, P<0.05; 2 g/kg, P<0.05) extracts of stigma showed significant activity against the acute inflammation (Tables 4 and 5).

Bottom Line: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent.The extracts exhibited antinociceptive activity against acetic acid induced writhing.We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran. hosseinzadehh@yahoo.com

ABSTRACT

Background: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent. We aimed to evaluate the antinociceptive and anti-inflammatory activity of saffron extracts in mice.

Results: We used aqueous and ethanolic maceration extracts of Crocus sativus L. stigma and petals. Antinociceptive activity was examined using the hot plate and writhing tests. The effect of extracts against acute inflammation was studied using xylene induced ear edema in mice. The activity of the extracts against chronic inflammation was assessed by formalin-induced edema in the rat paw. In the hot plate tests, intraperitoneal injection of both extracts showed no significant antinociceptive activity in mice. The extracts exhibited antinociceptive activity against acetic acid induced writhing. Naloxone partially blocked only the antinociceptive activity of the stigma aqueous extract. Only the stigma extracts showed weak to moderate effect against acute inflammation. In chronic inflammation, both aqueous and ethanolic stigma extracts, as well as ethanolic petal extract, exerted anti-inflammatory effects.

Conclusions: We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

Show MeSH
Related in: MedlinePlus