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Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice.

Hosseinzadeh H, Younesi HM - BMC Pharmacol. (2002)

Bottom Line: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent.The extracts exhibited antinociceptive activity against acetic acid induced writhing.We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran. hosseinzadehh@yahoo.com

ABSTRACT

Background: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent. We aimed to evaluate the antinociceptive and anti-inflammatory activity of saffron extracts in mice.

Results: We used aqueous and ethanolic maceration extracts of Crocus sativus L. stigma and petals. Antinociceptive activity was examined using the hot plate and writhing tests. The effect of extracts against acute inflammation was studied using xylene induced ear edema in mice. The activity of the extracts against chronic inflammation was assessed by formalin-induced edema in the rat paw. In the hot plate tests, intraperitoneal injection of both extracts showed no significant antinociceptive activity in mice. The extracts exhibited antinociceptive activity against acetic acid induced writhing. Naloxone partially blocked only the antinociceptive activity of the stigma aqueous extract. Only the stigma extracts showed weak to moderate effect against acute inflammation. In chronic inflammation, both aqueous and ethanolic stigma extracts, as well as ethanolic petal extract, exerted anti-inflammatory effects.

Conclusions: We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

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Effect of a subcutaneous injection of naloxone on the antinociceptive effect of intraperitoneally administered Crocus sativus petal ethanolic extract, morphine and diclofenac on acetic acid-induced writhing test in mice. Values are the mean ± S.E.M. of writhes number for 8 mice, ***P<0.001, compared to control (normal saline); +++P<0.001, compared to morphine plus naloxone, Tukey-Kramer test.
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Figure 2: Effect of a subcutaneous injection of naloxone on the antinociceptive effect of intraperitoneally administered Crocus sativus petal ethanolic extract, morphine and diclofenac on acetic acid-induced writhing test in mice. Values are the mean ± S.E.M. of writhes number for 8 mice, ***P<0.001, compared to control (normal saline); +++P<0.001, compared to morphine plus naloxone, Tukey-Kramer test.

Mentions: The aqueous and ethanolic extracts of C. sativus petal and stigma significantly reduce the number of mouse abdominal constrictions induced by a 0.7% acetic acid solution in a dose dependent manner (P<0.001, except for the dose of 0.36 g/kg of aqueous stigma which was P<0.01). Morphine and diclofenac induced a protection against abdominal constriction (P<0.001). Naloxone, (2 mg/kg, s.c.) pretreatment after i.p. injection of the extracts practically did not inhibit the antinociceptive activity of both extracts (figures 1, 2, 3, and 4). Only, the antinociceptive effect of aqueous stigma extract, 0.32 g/kg, was partially blocked by naloxone (figure 3). Naloxone completely antagonized the antinociceptive activity of morphine.


Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice.

Hosseinzadeh H, Younesi HM - BMC Pharmacol. (2002)

Effect of a subcutaneous injection of naloxone on the antinociceptive effect of intraperitoneally administered Crocus sativus petal ethanolic extract, morphine and diclofenac on acetic acid-induced writhing test in mice. Values are the mean ± S.E.M. of writhes number for 8 mice, ***P<0.001, compared to control (normal saline); +++P<0.001, compared to morphine plus naloxone, Tukey-Kramer test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC101384&req=5

Figure 2: Effect of a subcutaneous injection of naloxone on the antinociceptive effect of intraperitoneally administered Crocus sativus petal ethanolic extract, morphine and diclofenac on acetic acid-induced writhing test in mice. Values are the mean ± S.E.M. of writhes number for 8 mice, ***P<0.001, compared to control (normal saline); +++P<0.001, compared to morphine plus naloxone, Tukey-Kramer test.
Mentions: The aqueous and ethanolic extracts of C. sativus petal and stigma significantly reduce the number of mouse abdominal constrictions induced by a 0.7% acetic acid solution in a dose dependent manner (P<0.001, except for the dose of 0.36 g/kg of aqueous stigma which was P<0.01). Morphine and diclofenac induced a protection against abdominal constriction (P<0.001). Naloxone, (2 mg/kg, s.c.) pretreatment after i.p. injection of the extracts practically did not inhibit the antinociceptive activity of both extracts (figures 1, 2, 3, and 4). Only, the antinociceptive effect of aqueous stigma extract, 0.32 g/kg, was partially blocked by naloxone (figure 3). Naloxone completely antagonized the antinociceptive activity of morphine.

Bottom Line: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent.The extracts exhibited antinociceptive activity against acetic acid induced writhing.We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran. hosseinzadehh@yahoo.com

ABSTRACT

Background: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent. We aimed to evaluate the antinociceptive and anti-inflammatory activity of saffron extracts in mice.

Results: We used aqueous and ethanolic maceration extracts of Crocus sativus L. stigma and petals. Antinociceptive activity was examined using the hot plate and writhing tests. The effect of extracts against acute inflammation was studied using xylene induced ear edema in mice. The activity of the extracts against chronic inflammation was assessed by formalin-induced edema in the rat paw. In the hot plate tests, intraperitoneal injection of both extracts showed no significant antinociceptive activity in mice. The extracts exhibited antinociceptive activity against acetic acid induced writhing. Naloxone partially blocked only the antinociceptive activity of the stigma aqueous extract. Only the stigma extracts showed weak to moderate effect against acute inflammation. In chronic inflammation, both aqueous and ethanolic stigma extracts, as well as ethanolic petal extract, exerted anti-inflammatory effects.

Conclusions: We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

Show MeSH
Related in: MedlinePlus