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Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice.

Hosseinzadeh H, Younesi HM - BMC Pharmacol. (2002)

Bottom Line: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent.The extracts exhibited antinociceptive activity against acetic acid induced writhing.We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran. hosseinzadehh@yahoo.com

ABSTRACT

Background: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent. We aimed to evaluate the antinociceptive and anti-inflammatory activity of saffron extracts in mice.

Results: We used aqueous and ethanolic maceration extracts of Crocus sativus L. stigma and petals. Antinociceptive activity was examined using the hot plate and writhing tests. The effect of extracts against acute inflammation was studied using xylene induced ear edema in mice. The activity of the extracts against chronic inflammation was assessed by formalin-induced edema in the rat paw. In the hot plate tests, intraperitoneal injection of both extracts showed no significant antinociceptive activity in mice. The extracts exhibited antinociceptive activity against acetic acid induced writhing. Naloxone partially blocked only the antinociceptive activity of the stigma aqueous extract. Only the stigma extracts showed weak to moderate effect against acute inflammation. In chronic inflammation, both aqueous and ethanolic stigma extracts, as well as ethanolic petal extract, exerted anti-inflammatory effects.

Conclusions: We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

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Related in: MedlinePlus

Effect of stigma Crocus sativus aqueous and ethanolic extracts and diclofenac on formaldehyde induced arthritis in hind paw of rats. The inflammation was produced by subaponeurotic injection of 0.1 ml of 2% formaldehyde in the right hind paw of the rats on the first and third day. The animals were treated daily with the extracts or diclofenac intraperitoneally for 10 days. All agents were administered intraperitoneally. Each point represents the mean ± S.E.M. of change of paw size for 6 rats. Both extracts and diclofenac were effective compared to control (normal saline), Tukey-Kramer.
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Figure 10: Effect of stigma Crocus sativus aqueous and ethanolic extracts and diclofenac on formaldehyde induced arthritis in hind paw of rats. The inflammation was produced by subaponeurotic injection of 0.1 ml of 2% formaldehyde in the right hind paw of the rats on the first and third day. The animals were treated daily with the extracts or diclofenac intraperitoneally for 10 days. All agents were administered intraperitoneally. Each point represents the mean ± S.E.M. of change of paw size for 6 rats. Both extracts and diclofenac were effective compared to control (normal saline), Tukey-Kramer.

Mentions: In the chronic inflammation (formalin test), the aqueous petal extracts did not exhibit significant anti-inflammatory activity (P>0.05) but the ethanolic petal extract showed significant activity (figure 9). Both extracts of the stigma exerted significant anti-inflammatory activity (figure 10). In diclofenac and the ethanolic stigma extract (1.4 g/kg) groups; the hind paw edema of rat disappeared after 6 days (P<0.001) (figure 10). Diclofenac as well as the aqueous (0.8 g/kg) and ethanolic stigma extract (1.4 g/kg) showed anti-inflammatory effect on day one (P<0.05) (figure 10). On days 4 and 5, both diclofenac and the extracts did not demonstrate anti-inflammatory activity (P>0.05).


Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice.

Hosseinzadeh H, Younesi HM - BMC Pharmacol. (2002)

Effect of stigma Crocus sativus aqueous and ethanolic extracts and diclofenac on formaldehyde induced arthritis in hind paw of rats. The inflammation was produced by subaponeurotic injection of 0.1 ml of 2% formaldehyde in the right hind paw of the rats on the first and third day. The animals were treated daily with the extracts or diclofenac intraperitoneally for 10 days. All agents were administered intraperitoneally. Each point represents the mean ± S.E.M. of change of paw size for 6 rats. Both extracts and diclofenac were effective compared to control (normal saline), Tukey-Kramer.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC101384&req=5

Figure 10: Effect of stigma Crocus sativus aqueous and ethanolic extracts and diclofenac on formaldehyde induced arthritis in hind paw of rats. The inflammation was produced by subaponeurotic injection of 0.1 ml of 2% formaldehyde in the right hind paw of the rats on the first and third day. The animals were treated daily with the extracts or diclofenac intraperitoneally for 10 days. All agents were administered intraperitoneally. Each point represents the mean ± S.E.M. of change of paw size for 6 rats. Both extracts and diclofenac were effective compared to control (normal saline), Tukey-Kramer.
Mentions: In the chronic inflammation (formalin test), the aqueous petal extracts did not exhibit significant anti-inflammatory activity (P>0.05) but the ethanolic petal extract showed significant activity (figure 9). Both extracts of the stigma exerted significant anti-inflammatory activity (figure 10). In diclofenac and the ethanolic stigma extract (1.4 g/kg) groups; the hind paw edema of rat disappeared after 6 days (P<0.001) (figure 10). Diclofenac as well as the aqueous (0.8 g/kg) and ethanolic stigma extract (1.4 g/kg) showed anti-inflammatory effect on day one (P<0.05) (figure 10). On days 4 and 5, both diclofenac and the extracts did not demonstrate anti-inflammatory activity (P>0.05).

Bottom Line: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent.The extracts exhibited antinociceptive activity against acetic acid induced writhing.We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, PO Box 91775-1365, Mashhad, Iran. hosseinzadehh@yahoo.com

ABSTRACT

Background: Crocus sativus L. (saffron) is used in folk medicine, for example as an antiedematogenic agent. We aimed to evaluate the antinociceptive and anti-inflammatory activity of saffron extracts in mice.

Results: We used aqueous and ethanolic maceration extracts of Crocus sativus L. stigma and petals. Antinociceptive activity was examined using the hot plate and writhing tests. The effect of extracts against acute inflammation was studied using xylene induced ear edema in mice. The activity of the extracts against chronic inflammation was assessed by formalin-induced edema in the rat paw. In the hot plate tests, intraperitoneal injection of both extracts showed no significant antinociceptive activity in mice. The extracts exhibited antinociceptive activity against acetic acid induced writhing. Naloxone partially blocked only the antinociceptive activity of the stigma aqueous extract. Only the stigma extracts showed weak to moderate effect against acute inflammation. In chronic inflammation, both aqueous and ethanolic stigma extracts, as well as ethanolic petal extract, exerted anti-inflammatory effects.

Conclusions: We conclude that aqueous and ethanolic extracts of saffron stigma and petal have an antinociceptive effect, as well as acute and/or chronic anti-inflammatory activity.

Show MeSH
Related in: MedlinePlus