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Calculated 3D structure of the endochitinase NrChit1 from Nepenthes rafflesiana. The structure was suggested by the SWISS-MODEL web server upon comparative modelling using an endochitinase sequence from Hevea brasiliensis (NCPI, CAA09110; Swiss Prot, P23472) as the homologous template structure. The modelled residue range is from amino acids 1 to 266. A and B represent different views of the modelled structure.

Functional characterization of a class III acid endochitinase from the traps of the carnivorous pitcher plant genus, Nepenthes.

Rottloff S et al. - J. Exp. Bot. (2011)

Calculated 3D structure of the endochitinase NrChit1 from Nepenthes rafflesiana. The structure was suggested by the SWISS-MODEL web server upon comparative modelling using an endochitinase...

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getmorefigures.php?pmc=3170555&rFormat=json&query=the&uniq=1&req=5

Mainstream cigarette smoke apparatus in rats.

Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats.

Damasceno DC et al. - Diabetol Metab Syndr (2011)

Mainstream cigarette smoke apparatus in rats.

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getmorefigures.php?pmc=3170573&rFormat=json&query=the&uniq=1&req=5

Physical interaction between Tax and CYLD. (A) Cell lysates were prepared from the HTLV1-transformed C8166 cell line and subjected to IP using either a control pre-immune serum or anti-CYLD. The precipitated CYLD and its associated Tax were analyzed by IB. (B) HEK293 cells were transfected with HA-CYLD and Tax either in the absence (-) or presence (+) of IKKγ. The cell lysates were subjected to IP using anti-CYLD followed by IB to detect the precipitated CYLD and its associated Tax (top two panels). The cell lysates were also subjected to direct IB to monitor the expression of IKKγ and Tax. (C) HEK293 cells were transfected with HA-CYLD and Tax. The cells were stained with anti-HA (Y-11) and a mouse monoclonal anti-Tax antibody, followed with Texas red-conjugated donkey anti-rabbit Ig and FITC-conjugated donkey anti-mouse Ig. Cells were also counterstained with Hoechst 33258 for nuclear visualization. The expression of CYLD, Tax, Tax-CYLD merge, and nucleus (Hoechst) are shown. Note that the cytoplasmic, but not nuclear, Tax was colocalized with CYLD (Tax-CYLD merge, yellow color).

Mutual regulation between deubiquitinase CYLD and retroviral oncoprotein Tax.

Wu X et al. - Cell Biosci (2011)

Physical interaction between Tax and CYLD. (A) Cell lysates were prepared from the HTLV1-transformed C8166 cell line and subjected to IP using either a control pre-immune serum or anti-CYLD....

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getmorefigures.php?pmc=3170579&rFormat=json&query=the&uniq=1&req=5

Effect of CLE peptides on in vitro callus and root formation in M. truncatula. Leaf explants from line 2HA were plated onto P4 medium containing 10 μM NAA with or without 4 μM BAP and with or without further addition of CLE peptides at 10 μM. All CLE36 derivatives inhibited root initiation and growth except the biologically inactive 10 amino acid derivative. In addition, TDIF and CLV3 also inhibited root initiation and growth from the explants. Callus formation was also inhibited by CLE36 and CLV3, but not TDIF. The identity of the peptides and length in parentheses are indicated. Scale bar=10 mm.

Border sequences of Medicago truncatula CLE36 are specifically cleaved by endoproteases common to the extracellular fluids of Medicago and soybean.

Djordjevic MA et al. - J. Exp. Bot. (2011)

Effect of CLE peptides on in vitro callus and root formation in M. truncatula. Leaf explants from line 2HA were plated onto P4 medium containing 10 μM NAA with or without 4 μM BAP and...

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getmorefigures.php?pmc=3170558&rFormat=json&query=the&uniq=1&req=5

CT findings of the tumor. The mass in the right lung field with its inferior border abutting the diaphragm (arrow) increased in size over time. A At the first admission. B At 3 months, and C 12 months after the operation for lung cancer.

Post-traumatic diaphragmatic herniation of the liver, examined by positron emission tomography: case report.

Sato K et al. - World J Emerg Surg (2011)

CT findings of the tumor. The mass in the right lung field with its inferior border abutting the diaphragm (arrow) increased in size over time. A At the first admission. B At 3 months,...

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getmorefigures.php?pmc=3170580&rFormat=json&query=the&uniq=1&req=5

Subcellular localization of OsGMST1-GFP transiently expressed in rice protoplasts. (A, B) A rice protoplast cell expressing OsGMST1-GFP (A) and its DIC image (B), showing a punctuate expression pattern. Bars=10 μm. (C, D) A rice protoplast cell expressing GFP (C) as control and its DIC image (D), showing its distribution in nucleus, membrane, and cytoplasm. Bars=10 μm. (E–H) A rice protoplast cell expressing OsGMST1-GFP (E), Golgi localized ST-RFP (F) ,a merged image (G), and its DIC image (H). Bars=10 μm. (I–L) A rice protoplast expressing OsGMST1-GFP (I), stained by mitochondria dye MitoTracker Red (J), a merged image (K), and its DIC image (L). Bars=10 μm. (M–P) A rice protoplast cell expressing OsGMST1-GFP (M), the chlorophyll autofluorescence (N), a merged image (O), and its DIC image (P). Bars=10 μm. (This figure is available in colour at JXB online.)

Reduced expression of a gene encoding a Golgi localized monosaccharide transporter (OsGMST1) confers hypersensitivity to salt in rice (Oryza sativa).

Cao H et al. - J. Exp. Bot. (2011)

Subcellular localization of OsGMST1-GFP transiently expressed in rice protoplasts. (A, B) A rice protoplast cell expressing OsGMST1-GFP (A) and its DIC image (B), showing a punctuate...

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getmorefigures.php?pmc=3170556&rFormat=json&query=the&uniq=1&req=5

Comparison of wild-type and eve1-D plants at various developmental stages. (A–F) Phenotypes of 5-day-old wild-type (A) and eve1-D mutant (D) plants, 10-day-old wild-type (B) and eve1-D mutant (E) plants, and 15-day-old wild-type (C) and eve1-D mutant (F) plants. (G) A 25-day-old wild-type plant. (H) A 40-day-old wild-type plant. (I) A 25-day-old eve1-D plant. (J) A 40-day-old eve1-D plant. Bars=100mm in A–J.

Overexpression of EVE1, a novel ubiquitin family protein, arrests inflorescence stem development in Arabidopsis.

Hwang HJ et al. - J. Exp. Bot. (2011)

Comparison of wild-type and eve1-D plants at various developmental stages. (A–F) Phenotypes of 5-day-old wild-type (A) and eve1-D mutant (D) plants, 10-day-old wild-type (B) and eve1-D...

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getmorefigures.php?pmc=3170552&rFormat=json&query=the&uniq=1&req=5

Macroscopic, histochemical, and immunochemical analyses of NRCN formation. Germination and growth of maize caryopses (Merit) in (A) and (B) were under D-LS conditions with a heat shock of 40 °C at 84 h, as described in the Materials and methods. (A) Typical aspect of a 6.5-day-old seedling after growth under D-LS conditions and heat shock treatment. (B) Detail of the seedling in (A) showing two nodal roots protruding from the coleoptilar node. Germination and growth of maize caryopses in (C), (D), (E), and (F) was similar to the experiments described in Fig. 6B. At the end of the experiments, segments of tissue around the coleoptilar node were dissected for histochemical (C, D) or immunochemical (E, F) analysis as described in the Materials and methods. Images show transverse sections from: (C) a typical non-induced seedling lacking NR primordia in the region just above the coleoptilar node; (D) an induced seedling displaying one root primordium in the region just above the coleoptilar node; (E) a non-induced seedling showing the distribution of HSP101 and lacking any signs of NR primordia in the region just above the coleptilar node; (F) an induced seedling showing the distribution of HSP101 and displaying two NR primordia and one NR protruding from the coleoptile in the region just above the coleoptilar node. Notice that the entire NR that is protruding from the coleoptile is in the same plane of the tissue section allowing the appreciation of its procambial origin. Black arrows point to one of the vascular bundles of the coleoptile; white arrows show procambium of the second internode; c, coleoptile; m, medullar region. The scale bar in (C), (D), (E), and (F) represents 1 mm. For a more complete description of the tissue sections, see text in the Results section.

Role of HSP101 in the stimulation of nodal root development from the coleoptilar node by light and temperature in maize (Zea mays L.) seedlings.

López-Frías G et al. - J. Exp. Bot. (2011)

Macroscopic, histochemical, and immunochemical analyses of NRCN formation. Germination and growth of maize caryopses (Merit) in (A) and (B) were under D-LS conditions with a heat shock...

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getmorefigures.php?pmc=3170559&rFormat=json&query=the&uniq=1&req=5

Habitat of I. australis (a), uprooted specimen (b), leaf with sporangium (c), and transverse hand-cut sections of the green tips (d, e) and white bases (f, g) of leaves. Each granite outcrop supports numerous shallow pools that typically fill and evaporate throughout the winter and early spring (June to October), and then completely dry out in late spring/early summer, depending on rainfall patterns. Some pools host dense populations (>5000 plants m−2, see Fig. 3) of I. australis. Each plant typically had 6–10 leaves (b) with a white base (achlorophyllous) supporting the sporangium (c) and green tips (chlorophyllous). Cross-section micrographs of the leaves under bright field illumination (d, e) and ultraviolet light excitation, resulting in red chlorophyll autofluorescence (e, g), illustrate that in the green tips most cells contained chlorophyll (except the epidermal cells and those in the vascular bundles) and a well-developed cuticle (e), whereas there was no chlorophyll autofluorescence and no indication of a cuticle in the white bases (g).

In situ O2 dynamics in submerged Isoetes australis: varied leaf gas permeability influences underwater photosynthesis and internal O2.

Pedersen O et al. - J. Exp. Bot. (2011)

Habitat of I. australis (a), uprooted specimen (b), leaf with sporangium (c), and transverse hand-cut sections of the green tips (d, e) and white bases (f, g) of leaves. Each granite...

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getmorefigures.php?pmc=3170561&rFormat=json&query=the&uniq=1&req=5

Expression analysis of ENT1 by the use of corresponding promoter–GUS lines. (A–C) Two-d-old seedlings. (A) Whole seedling, (B) root tip, (C) cross-section of root elongation zone, (D) 10-d-old seedling, root hair zone; (E) developed leaf, arrows showing stained hydathodes, (F) flower, (G) anther with pollen, (H) germinated pollen.

Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis.

Bernard C et al. - J. Exp. Bot. (2011)

Expression analysis of ENT1 by the use of corresponding promoter–GUS lines. (A–C) Two-d-old seedlings. (A) Whole seedling, (B) root tip, (C) cross-section of root elongation zone,...

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getmorefigures.php?pmc=3170557&rFormat=json&query=the&uniq=1&req=5

Calculated 3D structure of the endochitinase NrChit1 from Nepenthes rafflesiana. The structure was suggested by the SWISS-MODEL web server upon comparative modelling using an endochitinase sequence from Hevea brasiliensis (NCPI, CAA09110; Swiss Prot, P23472) as the homologous template structure. The modelled residue range is from amino acids 1 to 266. A and B represent different views of the modelled structure.

Functional characterization of a class III acid endochitinase from the traps of the carnivorous pitcher plant genus, Nepenthes.

Rottloff S, Stieber R, Maischak H, Turini FG, Heubl G, Mithöfer A - J. Exp. Bot. (2011)

Calculated 3D structure of the endochitinase NrChit1 from Nepenthes rafflesiana. The structure was suggested by the SWISS-MODEL web server upon comparative modelling using an endochitinase...
Mainstream cigarette smoke apparatus in rats.

Effects of exposure to cigarette smoke prior to pregnancy in diabetic rats.

Damasceno DC, Sinzato YK, Lima PH, de Souza MS, Campos KE, Dallaqua B, Calderon IM, Rudge MV, Volpato GT - Diabetol Metab Syndr (2011)

Mainstream cigarette smoke apparatus in rats.
Physical interaction between Tax and CYLD. (A) Cell lysates were prepared from the HTLV1-transformed C8166 cell line and subjected to IP using either a control pre-immune serum or anti-CYLD. The precipitated CYLD and its associated Tax were analyzed by IB. (B) HEK293 cells were transfected with HA-CYLD and Tax either in the absence (-) or presence (+) of IKKγ. The cell lysates were subjected to IP using anti-CYLD followed by IB to detect the precipitated CYLD and its associated Tax (top two panels). The cell lysates were also subjected to direct IB to monitor the expression of IKKγ and Tax. (C) HEK293 cells were transfected with HA-CYLD and Tax. The cells were stained with anti-HA (Y-11) and a mouse monoclonal anti-Tax antibody, followed with Texas red-conjugated donkey anti-rabbit Ig and FITC-conjugated donkey anti-mouse Ig. Cells were also counterstained with Hoechst 33258 for nuclear visualization. The expression of CYLD, Tax, Tax-CYLD merge, and nucleus (Hoechst) are shown. Note that the cytoplasmic, but not nuclear, Tax was colocalized with CYLD (Tax-CYLD merge, yellow color).

Mutual regulation between deubiquitinase CYLD and retroviral oncoprotein Tax.

Wu X, Zhang M, Sun SC - Cell Biosci (2011)

Physical interaction between Tax and CYLD. (A) Cell lysates were prepared from the HTLV1-transformed C8166 cell line and subjected to IP using either a control pre-immune serum or anti-CYLD....
Effect of CLE peptides on in vitro callus and root formation in M. truncatula. Leaf explants from line 2HA were plated onto P4 medium containing 10 μM NAA with or without 4 μM BAP and with or without further addition of CLE peptides at 10 μM. All CLE36 derivatives inhibited root initiation and growth except the biologically inactive 10 amino acid derivative. In addition, TDIF and CLV3 also inhibited root initiation and growth from the explants. Callus formation was also inhibited by CLE36 and CLV3, but not TDIF. The identity of the peptides and length in parentheses are indicated. Scale bar=10 mm.

Border sequences of Medicago truncatula CLE36 are specifically cleaved by endoproteases common to the extracellular fluids of Medicago and soybean.

Djordjevic MA, Oakes M, Wong CE, Singh M, Bhalla P, Kusumawati L, Imin N - J. Exp. Bot. (2011)

Effect of CLE peptides on in vitro callus and root formation in M. truncatula. Leaf explants from line 2HA were plated onto P4 medium containing 10 μM NAA with or without 4 μM BAP and...
CT findings of the tumor. The mass in the right lung field with its inferior border abutting the diaphragm (arrow) increased in size over time. A At the first admission. B At 3 months, and C 12 months after the operation for lung cancer.

Post-traumatic diaphragmatic herniation of the liver, examined by positron emission tomography: case report.

Sato K, Orihashi K, Hamanaka Y, Mitsui N, Hirai S, Chatani N, Nishisaka T - World J Emerg Surg (2011)

CT findings of the tumor. The mass in the right lung field with its inferior border abutting the diaphragm (arrow) increased in size over time. A At the first admission. B At 3 months,...
Subcellular localization of OsGMST1-GFP transiently expressed in rice protoplasts. (A, B) A rice protoplast cell expressing OsGMST1-GFP (A) and its DIC image (B), showing a punctuate expression pattern. Bars=10 μm. (C, D) A rice protoplast cell expressing GFP (C) as control and its DIC image (D), showing its distribution in nucleus, membrane, and cytoplasm. Bars=10 μm. (E–H) A rice protoplast cell expressing OsGMST1-GFP (E), Golgi localized ST-RFP (F) ,a merged image (G), and its DIC image (H). Bars=10 μm. (I–L) A rice protoplast expressing OsGMST1-GFP (I), stained by mitochondria dye MitoTracker Red (J), a merged image (K), and its DIC image (L). Bars=10 μm. (M–P) A rice protoplast cell expressing OsGMST1-GFP (M), the chlorophyll autofluorescence (N), a merged image (O), and its DIC image (P). Bars=10 μm. (This figure is available in colour at JXB online.)

Reduced expression of a gene encoding a Golgi localized monosaccharide transporter (OsGMST1) confers hypersensitivity to salt in rice (Oryza sativa).

Cao H, Guo S, Xu Y, Jiang K, Jones AM, Chong K - J. Exp. Bot. (2011)

Subcellular localization of OsGMST1-GFP transiently expressed in rice protoplasts. (A, B) A rice protoplast cell expressing OsGMST1-GFP (A) and its DIC image (B), showing a punctuate...
Comparison of wild-type and eve1-D plants at various developmental stages. (A–F) Phenotypes of 5-day-old wild-type (A) and eve1-D mutant (D) plants, 10-day-old wild-type (B) and eve1-D mutant (E) plants, and 15-day-old wild-type (C) and eve1-D mutant (F) plants. (G) A 25-day-old wild-type plant. (H) A 40-day-old wild-type plant. (I) A 25-day-old eve1-D plant. (J) A 40-day-old eve1-D plant. Bars=100mm in A–J.

Overexpression of EVE1, a novel ubiquitin family protein, arrests inflorescence stem development in Arabidopsis.

Hwang HJ, Kim H, Jeong YM, Choi MY, Lee SY, Kim SG - J. Exp. Bot. (2011)

Comparison of wild-type and eve1-D plants at various developmental stages. (A–F) Phenotypes of 5-day-old wild-type (A) and eve1-D mutant (D) plants, 10-day-old wild-type (B) and eve1-D...
Macroscopic, histochemical, and immunochemical analyses of NRCN formation. Germination and growth of maize caryopses (Merit) in (A) and (B) were under D-LS conditions with a heat shock of 40 °C at 84 h, as described in the Materials and methods. (A) Typical aspect of a 6.5-day-old seedling after growth under D-LS conditions and heat shock treatment. (B) Detail of the seedling in (A) showing two nodal roots protruding from the coleoptilar node. Germination and growth of maize caryopses in (C), (D), (E), and (F) was similar to the experiments described in Fig. 6B. At the end of the experiments, segments of tissue around the coleoptilar node were dissected for histochemical (C, D) or immunochemical (E, F) analysis as described in the Materials and methods. Images show transverse sections from: (C) a typical non-induced seedling lacking NR primordia in the region just above the coleoptilar node; (D) an induced seedling displaying one root primordium in the region just above the coleoptilar node; (E) a non-induced seedling showing the distribution of HSP101 and lacking any signs of NR primordia in the region just above the coleptilar node; (F) an induced seedling showing the distribution of HSP101 and displaying two NR primordia and one NR protruding from the coleoptile in the region just above the coleoptilar node. Notice that the entire NR that is protruding from the coleoptile is in the same plane of the tissue section allowing the appreciation of its procambial origin. Black arrows point to one of the vascular bundles of the coleoptile; white arrows show procambium of the second internode; c, coleoptile; m, medullar region. The scale bar in (C), (D), (E), and (F) represents 1 mm. For a more complete description of the tissue sections, see text in the Results section.

Role of HSP101 in the stimulation of nodal root development from the coleoptilar node by light and temperature in maize (Zea mays L.) seedlings.

López-Frías G, Martínez LM, Ponce G, Cassab GI, Nieto-Sotelo J - J. Exp. Bot. (2011)

Macroscopic, histochemical, and immunochemical analyses of NRCN formation. Germination and growth of maize caryopses (Merit) in (A) and (B) were under D-LS conditions with a heat shock...
Habitat of I. australis (a), uprooted specimen (b), leaf with sporangium (c), and transverse hand-cut sections of the green tips (d, e) and white bases (f, g) of leaves. Each granite outcrop supports numerous shallow pools that typically fill and evaporate throughout the winter and early spring (June to October), and then completely dry out in late spring/early summer, depending on rainfall patterns. Some pools host dense populations (>5000 plants m−2, see Fig. 3) of I. australis. Each plant typically had 6–10 leaves (b) with a white base (achlorophyllous) supporting the sporangium (c) and green tips (chlorophyllous). Cross-section micrographs of the leaves under bright field illumination (d, e) and ultraviolet light excitation, resulting in red chlorophyll autofluorescence (e, g), illustrate that in the green tips most cells contained chlorophyll (except the epidermal cells and those in the vascular bundles) and a well-developed cuticle (e), whereas there was no chlorophyll autofluorescence and no indication of a cuticle in the white bases (g).

In situ O2 dynamics in submerged Isoetes australis: varied leaf gas permeability influences underwater photosynthesis and internal O2.

Pedersen O, Pulido C, Rich SM, Colmer TD - J. Exp. Bot. (2011)

Habitat of I. australis (a), uprooted specimen (b), leaf with sporangium (c), and transverse hand-cut sections of the green tips (d, e) and white bases (f, g) of leaves. Each granite...
Expression analysis of ENT1 by the use of corresponding promoter–GUS lines. (A–C) Two-d-old seedlings. (A) Whole seedling, (B) root tip, (C) cross-section of root elongation zone, (D) 10-d-old seedling, root hair zone; (E) developed leaf, arrows showing stained hydathodes, (F) flower, (G) anther with pollen, (H) germinated pollen.

Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis.

Bernard C, Traub M, Kunz HH, Hach S, Trentmann O, Möhlmann T - J. Exp. Bot. (2011)

Expression analysis of ENT1 by the use of corresponding promoter–GUS lines. (A–C) Two-d-old seedlings. (A) Whole seedling, (B) root tip, (C) cross-section of root elongation zone,...

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