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Subcellular localization of VCP/p97 in undifferentiated controls (U937), differentiated populations (3d, 7d, 14d) and retrodifferentiated U937 cells (Retro) by immunofluorescence. A. VCP/p97 was visualized by a secondary FITC-conjugated rabbit anti-goat antibody (VCP-Ab). The nuclei were stained with the DNA-intercalating dye Hoechst 33258. B. The VCP/p97 antibody was neutralized with a specific blocking peptide and incubated with a secondary FITC-conjugated rabbit anti-goat antibody (VCP-Ab + VCP-peptide). The nuclei were stained with the DNA-intercalating dye Hoechst 33258.

The differentiation/retrodifferentiation program of human U937 leukemia cells is accompanied by changes of VCP/p97.

Bertram C et al. - (2008)

Subcellular localization of VCP/p97 in undifferentiated controls (U937), differentiated populations (3d, 7d, 14d) and retrodifferentiated U937 cells (Retro) by immunofluorescence. A....

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getmorefigures.php?pmc=2277395&rFormat=json&query=the&uniq=1&req=5

Characterization of RBC and nRBC cellular fractions. (A) Appearance of cell populations following density gradient centrifugation, along with control density marker beads. (B) FACSAria (BD Biosciences) profile of RBC and nRBCs after propidium iodide labeling of dead cells. The number of small (FSC), agranular (SSC) cells is greater in the nRBC fraction; >90% of cells from both populations are viable based on propidium iodide exclusion and Trypan blue exclusion (not shown). (C) nRBCs have a large nuclear volume, and smaller size (Giemsa), are benzidine negative and PAS positive. Bar = 20 μm.

Expression profiling of circulating non-red blood cells in embryonic blood.

McIntyre BA et al. - (2008)

Characterization of RBC and nRBC cellular fractions. (A) Appearance of cell populations following density gradient centrifugation, along with control density marker beads. (B) FACSAria...

Related in: Results Collection MedlinePlus

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getmorefigures.php?pmc=2277405&rFormat=json&query=the&uniq=1&req=5

(a&b) – CT Scan: Bilateral hydronephrosis with associated caecal mass (arrow showing hydronephrosis).

Adenocarcinoma of the appendix presenting as bilateral ureteric obstruction.

Ahmed K et al. - (2008)

(a&b) – CT Scan: Bilateral hydronephrosis with associated caecal mass (arrow showing hydronephrosis).

Related in: Results Collection MedlinePlus

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getmorefigures.php?pmc=2277416&rFormat=json&query=the&uniq=1&req=5

Magnetic resonance image of intraneural hemangioma. (A) Sagittal T1 (B) T2 (C) axial T1 and (D) T2 (E) fat suppression images demonstrating an 3 × 2 × 1.5 cm lesion in the volar aspect of the right wrist.

Intraneural hemangioma of the median nerve: A case report.

Doğramaci Y et al. - (2008)

Magnetic resonance image of intraneural hemangioma. (A) Sagittal T1 (B) T2 (C) axial T1 and (D) T2 (E) fat suppression images demonstrating an 3 × 2 × 1.5 cm lesion in the volar aspect...

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getmorefigures.php?pmc=2277418&rFormat=json&query=the&uniq=1&req=5

Lbx2 expression in embryonic an neonatal testis. Six μm paraffin sections of paraformaldehyde-fixed mouse testis were probed with DIG-labeled antisense (B and D) and sense (A) Lbx2 cRNA probes in in situ hybridization experiments. Lbx2 mRNA was detected by immunostaining using an alkaline phosphatase-coupled anti-DIG antibody (appears as a blue-purplish staining). Tissues were counterstained with Neutral Red to visualize nuclei. Lbx2 expression in the testis was assessed at E18 (B) and P1 (D). Immunohistochemistry for the Sertoli cell marker MIS was performed on testis sections at E18 (C) and P1 (E) as described in Methods and revealed using AEC (shows as a red-brownish staining). Expression of Lbx2 is evident within the cytoplasm of Sertoli cells by comparison with MIS staining. A weak but consistent Lbx2 staining is also observed in interstitial cells. Gonocytes are not labeled. No significant signal was detected with the sense probe (A). Go: gonocytes, S: Sertoli cells, I: interstitial cells. Magnifications: 200× (D, E), 400× (A-C).

Expression of the Ladybird-like homeobox 2 transcription factor in the developing mouse testis and epididymis.

Moisan V et al. - (2008)

Lbx2 expression in embryonic an neonatal testis. Six μm paraffin sections of paraformaldehyde-fixed mouse testis were probed with DIG-labeled antisense (B and D) and sense (A) Lbx2 cRNA...

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getmorefigures.php?pmc=2277406&rFormat=json&query=the&uniq=1&req=5

Micrographs depicting SSeCKS colocalization with myelination markers (CNPase and Pzero). (A) SSeCKS (red) and CNPase (green) in the lumbar spinal cord dorsal horn. The labeling appears discrete with minimal colocalization. (B) SSeCKS (red) and Pzero (green) in the L4 dorsal root ganglia. A lack of co-localization is observed and Pzero can be seen localized to putative axonal elements (arrow). (C) SSeCKS (red) and Pzero (green) in the sciatic nerve. As in the dorsal root ganglia, a lack of co-localization is observed. Both SSeCKS and Pzero can be seen localized to axonal elements. (D) SSeCKS (red) and Pzero (green) in glabrous skin of the hind-paw, fibers displaying colocalization (yellow) can be observed (arrow).

Localization of SSeCKS in unmyelinated primary sensory neurons.

Irmen CP et al. - (2008)

Micrographs depicting SSeCKS colocalization with myelination markers (CNPase and Pzero). (A) SSeCKS (red) and CNPase (green) in the lumbar spinal cord dorsal horn. The labeling appears...

Related in: Results Collection MedlinePlus

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getmorefigures.php?pmc=2277419&rFormat=json&query=the&uniq=1&req=5

Microscopic photographs of cells exposed to selected doxorubicin concentrations. Photographs are taken at a magnification of ten times. C1 = 0.01 μg ml−1, C2 = 0.04 μg ml−1, C3 = 0.5 μg ml−1, C4 = 3.0 μg ml−1, C5 = 10 μg ml−1.

Effects of temperature and doxorubicin exposure on keratinocyte damage in vitro.

Janssen FP et al. - (2008)

Microscopic photographs of cells exposed to selected doxorubicin concentrations. Photographs are taken at a magnification of ten times. C1 = 0.01 μg ml−1, C2 = 0.04 μg ml−1,...

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getmorefigures.php?pmc=2277440&rFormat=json&query=the&uniq=1&req=5

Hematoxylin and eosin stained slides of formalin-fixed, paraffin-embedded tissue sample blocks of breast tumor tissue and metastatic breast tumor tissue in lymph node. Panel A (father): metastatic ductal carcinoma of breast in axillary lymph node. The tumor is almost completely replacing the normal tissue in this 2-cm node. Note the rim of residual subcapsular lymphoid tissue. Panel B (mother): invasive moderately differentiated ductal carcinoma of breast. Note the prominent lymphocytic response. Panel C (daughter): invasive and in situ lobular carcinoma of breast. Only a portion of the round edge of a lobule containing lobular carcinoma in situ is seen here. Magnification is 200X.

Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter.

Etkind PR et al. - (2008)

Hematoxylin and eosin stained slides of formalin-fixed, paraffin-embedded tissue sample blocks of breast tumor tissue and metastatic breast tumor tissue in lymph node. Panel A (father):...

Related in: Results Collection MedlinePlus

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getmorefigures.php?pmc=2277433&rFormat=json&query=the&uniq=1&req=5

(A) After intraoperative injection of Patent blue dye around liver metastasis in patient 18, the metinel node in the hepatoduodenal ligament is blue. (B) Preoperative percutaneous injection of 4 × 15 MBq Tc-nanokolloid around ovarian cancer groin local recurrence (patient 8) demonstrates a medially and distally situated metinel node after 10 minutes.

Metinel node--the first lymph node draining a metastasis--contains tumor-reactive lymphocytes.

Dahl K et al. - (2008)

(A) After intraoperative injection of Patent blue dye around liver metastasis in patient 18, the metinel node in the hepatoduodenal ligament is blue. (B) Preoperative percutaneous injection...

Related in: Results Collection MedlinePlus

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getmorefigures.php?pmc=2277445&rFormat=json&query=the&uniq=1&req=5

Expression of Hey2 in the developing inner ear. Otocyst (A-B) or cochlear sections (C-J) from E11.5 (A-B), E14.5 (C-J) were hybridized with RNA probes for Isl1 (A,C), Hey2 (B,D,I), Hes1 (J), Hes6 (E), Hey1 (F), and Oto90 (G). Arrowheads in A-B indicate the domain for sensory and neuronal lineages in the otocyst; the bracket in B marks the expression domain of Hey2; brackets in C-J indicate the primordial organ of Corti at E14.5. (K-M) Cochlear sections from an E14.5 Math1-/- embryo were probed for Hes6 (K), Isl1 (L), and Hey2 (M). Brackets indicate the developing sensory organ that was determined by careful comparison of adjacent sections with molecular markers for the region. The sides of the cochlear duct toward the center and the periphery of the spiraled cochlea are designated as the medial and lateral sides, respectively, and are indicated. Scale bars: 10 μm for A-B, C-G, H-J, K-M.

Hey2 functions in parallel with Hes1 and Hes5 for mammalian auditory sensory organ development.

Li S et al. - (2008)

Expression of Hey2 in the developing inner ear. Otocyst (A-B) or cochlear sections (C-J) from E11.5 (A-B), E14.5 (C-J) were hybridized with RNA probes for Isl1 (A,C), Hey2 (B,D,I), Hes1...

Related in: Results Collection MedlinePlus

View Article: PubMed Central HTML PubMed

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getmorefigures.php?pmc=2277407&rFormat=json&query=the&uniq=1&req=5

Subcellular localization of VCP/p97 in undifferentiated controls (U937), differentiated populations (3d, 7d, 14d) and retrodifferentiated U937 cells (Retro) by immunofluorescence. A. VCP/p97 was visualized by a secondary FITC-conjugated rabbit anti-goat antibody (VCP-Ab). The nuclei were stained with the DNA-intercalating dye Hoechst 33258. B. The VCP/p97 antibody was neutralized with a specific blocking peptide and incubated with a secondary FITC-conjugated rabbit anti-goat antibody (VCP-Ab + VCP-peptide). The nuclei were stained with the DNA-intercalating dye Hoechst 33258.

The differentiation/retrodifferentiation program of human U937 leukemia cells is accompanied by changes of VCP/p97.

Bertram C, von Neuhoff N, Skawran B, Steinemann D, Schlegelberger B, Hass R - (2008)

Subcellular localization of VCP/p97 in undifferentiated controls (U937), differentiated populations (3d, 7d, 14d) and retrodifferentiated U937 cells (Retro) by immunofluorescence. A....
Characterization of RBC and nRBC cellular fractions. (A) Appearance of cell populations following density gradient centrifugation, along with control density marker beads. (B) FACSAria (BD Biosciences) profile of RBC and nRBCs after propidium iodide labeling of dead cells. The number of small (FSC), agranular (SSC) cells is greater in the nRBC fraction; >90% of cells from both populations are viable based on propidium iodide exclusion and Trypan blue exclusion (not shown). (C) nRBCs have a large nuclear volume, and smaller size (Giemsa), are benzidine negative and PAS positive. Bar = 20 μm.

Expression profiling of circulating non-red blood cells in embryonic blood.

McIntyre BA, Alev C, Tarui H, Jakt LM, Sheng G - (2008)

Characterization of RBC and nRBC cellular fractions. (A) Appearance of cell populations following density gradient centrifugation, along with control density marker beads. (B) FACSAria...
(a&b) – CT Scan: Bilateral hydronephrosis with associated caecal mass (arrow showing hydronephrosis).

Adenocarcinoma of the appendix presenting as bilateral ureteric obstruction.

Ahmed K, Hoque R, El-Tawil S, Khan MS, George ML - (2008)

(a&b) – CT Scan: Bilateral hydronephrosis with associated caecal mass (arrow showing hydronephrosis).
Magnetic resonance image of intraneural hemangioma. (A) Sagittal T1 (B) T2 (C) axial T1 and (D) T2 (E) fat suppression images demonstrating an 3 × 2 × 1.5 cm lesion in the volar aspect of the right wrist.

Intraneural hemangioma of the median nerve: A case report.

Doğramaci Y, Kalaci A, Sevinç TT, Yanat AN - (2008)

Magnetic resonance image of intraneural hemangioma. (A) Sagittal T1 (B) T2 (C) axial T1 and (D) T2 (E) fat suppression images demonstrating an 3 × 2 × 1.5 cm lesion in the volar aspect...
Lbx2 expression in embryonic an neonatal testis. Six μm paraffin sections of paraformaldehyde-fixed mouse testis were probed with DIG-labeled antisense (B and D) and sense (A) Lbx2 cRNA probes in in situ hybridization experiments. Lbx2 mRNA was detected by immunostaining using an alkaline phosphatase-coupled anti-DIG antibody (appears as a blue-purplish staining). Tissues were counterstained with Neutral Red to visualize nuclei. Lbx2 expression in the testis was assessed at E18 (B) and P1 (D). Immunohistochemistry for the Sertoli cell marker MIS was performed on testis sections at E18 (C) and P1 (E) as described in Methods and revealed using AEC (shows as a red-brownish staining). Expression of Lbx2 is evident within the cytoplasm of Sertoli cells by comparison with MIS staining. A weak but consistent Lbx2 staining is also observed in interstitial cells. Gonocytes are not labeled. No significant signal was detected with the sense probe (A). Go: gonocytes, S: Sertoli cells, I: interstitial cells. Magnifications: 200× (D, E), 400× (A-C).

Expression of the Ladybird-like homeobox 2 transcription factor in the developing mouse testis and epididymis.

Moisan V, Bomgardner D, Tremblay JJ - (2008)

Lbx2 expression in embryonic an neonatal testis. Six μm paraffin sections of paraformaldehyde-fixed mouse testis were probed with DIG-labeled antisense (B and D) and sense (A) Lbx2 cRNA...
Micrographs depicting SSeCKS colocalization with myelination markers (CNPase and Pzero). (A) SSeCKS (red) and CNPase (green) in the lumbar spinal cord dorsal horn. The labeling appears discrete with minimal colocalization. (B) SSeCKS (red) and Pzero (green) in the L4 dorsal root ganglia. A lack of co-localization is observed and Pzero can be seen localized to putative axonal elements (arrow). (C) SSeCKS (red) and Pzero (green) in the sciatic nerve. As in the dorsal root ganglia, a lack of co-localization is observed. Both SSeCKS and Pzero can be seen localized to axonal elements. (D) SSeCKS (red) and Pzero (green) in glabrous skin of the hind-paw, fibers displaying colocalization (yellow) can be observed (arrow).

Localization of SSeCKS in unmyelinated primary sensory neurons.

Irmen CP, Siegel SM, Carr PA - (2008)

Micrographs depicting SSeCKS colocalization with myelination markers (CNPase and Pzero). (A) SSeCKS (red) and CNPase (green) in the lumbar spinal cord dorsal horn. The labeling appears...
Microscopic photographs of cells exposed to selected doxorubicin concentrations. Photographs are taken at a magnification of ten times. C1 = 0.01 μg ml−1, C2 = 0.04 μg ml−1, C3 = 0.5 μg ml−1, C4 = 3.0 μg ml−1, C5 = 10 μg ml−1.

Effects of temperature and doxorubicin exposure on keratinocyte damage in vitro.

Janssen FP, Bouten CV, van Leeuwen GM, van Steenhoven AA - (2008)

Microscopic photographs of cells exposed to selected doxorubicin concentrations. Photographs are taken at a magnification of ten times. C1 = 0.01 μg ml−1, C2 = 0.04 μg ml−1,...
Hematoxylin and eosin stained slides of formalin-fixed, paraffin-embedded tissue sample blocks of breast tumor tissue and metastatic breast tumor tissue in lymph node. Panel A (father): metastatic ductal carcinoma of breast in axillary lymph node. The tumor is almost completely replacing the normal tissue in this 2-cm node. Note the rim of residual subcapsular lymphoid tissue. Panel B (mother): invasive moderately differentiated ductal carcinoma of breast. Note the prominent lymphocytic response. Panel C (daughter): invasive and in situ lobular carcinoma of breast. Only a portion of the round edge of a lobule containing lobular carcinoma in situ is seen here. Magnification is 200X.

Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter.

Etkind PR, Stewart AF, Wiernik PH - (2008)

Hematoxylin and eosin stained slides of formalin-fixed, paraffin-embedded tissue sample blocks of breast tumor tissue and metastatic breast tumor tissue in lymph node. Panel A (father):...
(A) After intraoperative injection of Patent blue dye around liver metastasis in patient 18, the metinel node in the hepatoduodenal ligament is blue. (B) Preoperative percutaneous injection of 4 × 15 MBq Tc-nanokolloid around ovarian cancer groin local recurrence (patient 8) demonstrates a medially and distally situated metinel node after 10 minutes.

Metinel node--the first lymph node draining a metastasis--contains tumor-reactive lymphocytes.

Dahl K, Karlsson M, Marits P, Hoffstedt A, Winqvist O, Thörn M - (2008)

(A) After intraoperative injection of Patent blue dye around liver metastasis in patient 18, the metinel node in the hepatoduodenal ligament is blue. (B) Preoperative percutaneous injection...
Expression of Hey2 in the developing inner ear. Otocyst (A-B) or cochlear sections (C-J) from E11.5 (A-B), E14.5 (C-J) were hybridized with RNA probes for Isl1 (A,C), Hey2 (B,D,I), Hes1 (J), Hes6 (E), Hey1 (F), and Oto90 (G). Arrowheads in A-B indicate the domain for sensory and neuronal lineages in the otocyst; the bracket in B marks the expression domain of Hey2; brackets in C-J indicate the primordial organ of Corti at E14.5. (K-M) Cochlear sections from an E14.5 Math1-/- embryo were probed for Hes6 (K), Isl1 (L), and Hey2 (M). Brackets indicate the developing sensory organ that was determined by careful comparison of adjacent sections with molecular markers for the region. The sides of the cochlear duct toward the center and the periphery of the spiraled cochlea are designated as the medial and lateral sides, respectively, and are indicated. Scale bars: 10 μm for A-B, C-G, H-J, K-M.

Hey2 functions in parallel with Hes1 and Hes5 for mammalian auditory sensory organ development.

Li S, Mark S, Radde-Gallwitz K, Schlisner R, Chin MT, Chen P - (2008)

Expression of Hey2 in the developing inner ear. Otocyst (A-B) or cochlear sections (C-J) from E11.5 (A-B), E14.5 (C-J) were hybridized with RNA probes for Isl1 (A,C), Hey2 (B,D,I), Hes1...

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