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Observation of flocs by SEM. Mixed flocculation of Chlorella sorokiniana with CSSB-3 (a). (b) is a magnification of (a).
Imase M, Watanabe K, Aoyagi H, Tanaka H - FEMS Microbiol. Ecol. (2008)
Observation of flocs by SEM. Mixed flocculation of Chlorella sorokiniana with CSSB-3 (a). (b) is a magnification of (a).
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getmorefigures.php?pmc=2259099&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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Immunohistochemical staining for EphB4 and ephrinB2 in ovarian cancers (original magnification × 200). A representative case of clear cell carcinoma of the right ovary is shown. Rabbit EphB4 and ephrinB2 antibodies (Santa Cruz) were used at dilutions of 1 : 100 and 1 : 75, respectively, as primary antibodies. Dark brown staining represents positive for EphB4 and ephrinB2 antigen. Bars=100 μm.
Alam SM, Fujimoto J, Jahan I, Sato E, Tamaya T - Br. J. Cancer (2008)
Immunohistochemical staining for EphB4 and ephrinB2 in ovarian cancers (original magnification Γ— 200). A representative case of clear cell carcinoma of the right ovary is shown. Rabbit...
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getmorefigures.php?pmc=2259170&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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Immunohistochemical analysis of LAT1 in NSCLC. (A) Positive staining of LAT1 expression in squamous cell carcinoma. The score of LAT1 immunostaining was grade 4 and its immunostaining pattern was membranous. (B) LAT1 expression in squamous cell carcinoma with staining score of grade 2. (C) LAT1 expression in adenocarcinoma with staining score of grade 3. (D) LAT1 expression in large cell carcinoma with staining score of grade 4.
Kaira K, Oriuchi N, Imai H, Shimizu K, Yanagitani N, Sunaga N, Hisada T, et al. - Br. J. Cancer (2008)
Immunohistochemical analysis of LAT1 in NSCLC. (A) Positive staining of LAT1 expression in squamous cell carcinoma. The score of LAT1 immunostaining was grade 4 and its immunostaining...
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getmorefigures.php?pmc=2259171&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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CD44+CD24− cells express genes common to other stem-like cells and are genotypically distinct from both total LNCaP and CD44+CD24−-depleted cells. (A) CD44+CD24− cells, CD44+CD24−-depleted cells, and total LNCaP cells were analysed using microarrays for genes expressed by other stem-like cells. CD44+CD24− cells show higher expression of the genes analysed. (B) The relationship of CD44+CD24− cells, CD44+CD24−-depleted cells, and total LNCaP cells based on gene expression of 5713 genes is shown. While total LNCaP and CD44+CD24−-depleted cells remain together on a single branch, CD44+CD24− cells form a distinct branch, indicating a high degree of difference in their expression patterns for these genes. (C) The top 20 functional categories of the genes showing an equal or greater than two-fold change in CD44+CD24− cells as compared with both CD44+CD24−-depleted cells and the total LNCaP cells. The number of genes in each category is indicated in parentheses.
Hurt EM, Kawasaki BT, Klarmann GJ, Thomas SB, Farrar WL - Br. J. Cancer (2008)
CD44+CD24βˆ’ cells express genes common to other stem-like cells and are genotypically distinct from both total LNCaP and CD44+CD24βˆ’-depleted cells. (A) CD44+CD24βˆ’ cells, CD44+CD24βˆ’-depleted...
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getmorefigures.php?pmc=2259168&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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(A) Dkk-1 expression in cells distant from tumoral foci (T) in bone with osteoblasts (arrow) and stromal cells (arrow head) exhibiting membrane and cytoplasmic labelling. Other cells appearing in black at the top of the image are siderophages (S). (B) the negative control section stained with the same concentration of control antibody, note that a group of osteoblasts (arrows) is negative. (C) RANKL expression near tumour foci in bone. Osteoblasts (arrow) and stromal cells (arrow head) are RANKL+. Tumour foci are RANKL negative. (D) The negative control section stained with the same concentration of control antibody, note that a group of osteoblasts (arrow) is negative. (E) Cathepsin K expression near tumoral foci (T) in bone. Cathepsin K+ multi- and mononucleated cells (arrow head) have a diffuse and faint cytoplasmic labelling. Both can be found in marrow spaces at a distance from bone surfaces. A siderophage (iron-loaded macrophage) is also evidenced (S). (F) The negative control section stained with the same concentration of control antibody; note that an osteoclasts (arrow) is negative. For all images, the original magnification is × 400.
Blouin S, BaslΓ© MF, Chappard D - Br. J. Cancer (2008)
(A) Dkk-1 expression in cells distant from tumoral foci (T) in bone with osteoblasts (arrow) and stromal cells (arrow head) exhibiting membrane and cytoplasmic labelling. Other cells...
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getmorefigures.php?pmc=2259183&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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Pattern of positive staining of TPO, HBME, and DPP4. (A) Staining of TPO in benign follicular adenoma: brown granular deposit all over the cytoplasm and around the nuclei. (B) Staining of HBME in papillary cancer: brown reaction predominant on cellular membranes. (C) Staining of DPP4 in papillary cancer: contrasted red reaction within cytoplasm and along membranes.
de Micco C, Savchenko V, Giorgi R, Sebag F, Henry JF - Br. J. Cancer (2008)
Pattern of positive staining of TPO, HBME, and DPP4. (A) Staining of TPO in benign follicular adenoma: brown granular deposit all over the cytoplasm and around the nuclei. (B) Staining...
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getmorefigures.php?pmc=2259194&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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Histopathological and clinical analyses of 4NQO-treated tongues from MMP-8 KO and C57BL/6 mice. (A) Normal C57BL/6 male mouse mucosa stained with hematoxylin and eosin. Clinical tongue on the right. (B) MMP-8 KO male with dysplasia. (C) MMP-8 KO females with invasive SCC. (D) MMP-8 KO females with invasive SCC. Scale bar=200 μm.
Korpi JT, Kervinen V, MÀklin H, VÀÀnÀnen A, Lahtinen M, LÀÀrÀ E, et al. - Br. J. Cancer (2008)
Histopathological and clinical analyses of 4NQO-treated tongues from MMP-8 KO and C57BL/6 mice. (A) Normal C57BL/6 male mouse mucosa stained with hematoxylin and eosin. Clinical tongue...
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getmorefigures.php?pmc=2259187&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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Anchoring paralogous sequences onto the physical map by 6-D pools and confirmed by FISH. (a) Paralogous sequences of PCR product. The pink arrow shows nucleotide variations in a sequence read. (b) Alignment between homoeologous BACs. Blue lines show some regions with inconsistent alignment between the two BACs (c) FISH mapping of homoeologous BACs (Gm_WBb0130B22 and Gm_WBb0060P19) anchored by marker 13617. The leftmost panel shows the entire karyotype of a chromosome spread prepared from mitotic root tip cells. The probe for BAC Gm_WBb0130B22 (abbreviated as 130B22) was labeled with green fluorophore and Gm_WBb0060P19 (abbreviated as 60P19) was labeled with red fluorophore; overlap therefore appears as yellow. Right panels are enlarged fields in which the left panels show the merged channels, the middle panels show the green (60P19) channel and the right panels show the red (130B22) channel. The 60P19 probe localizes to two pairs of strong spots (corresponding to the duplicated chromatids of homologous chromosomes) as well as to two pairs of less intense signal that overlap with the 130B22 loci. The yellow arrows indicate overlapping spots.
Wu X, Zhong G, Findley SD, Cregan P, Stacey G, Nguyen HT - BMC Genomics (2008)
Anchoring paralogous sequences onto the physical map by 6-D pools and confirmed by FISH. (a) Paralogous sequences of PCR product. The pink arrow shows nucleotide variations in a sequence...
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getmorefigures.php?pmc=2259328&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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Representative gel view and MCA using sex-specific primers of S. c. hoya. PCR products with 185- and 112-bp amplified by (A) CHD-W-F/R and (B) CHD-Z-F/R primers in different PCR-wells were run in 4% agarose gel, respectively. The arrow of Fig. 4A indicates the remaining primers or primer-dimers of PCR. Non-specific products of W-185 indicated with star symbols were only occurred in male samples. (C, D) demonstration for high-throughput molecular sexing of multiple S. c. hoya samples using MCA. All data are performed in duplicate. (C) 7 females (Bds 12, 14, 15, 17, 19, 20, and 23) and (D) 6 males (Bds 13, 16, 18, 21, 22, and 24) were included. Z-112 and W-185 represent the primer mixtures for CHD-Z-F/CHD-Z-R (112-bp) and CHD-W-F/CHD-W-R (185-bp). The Tm value for Z-112 and W-185 is 75.0°C and 79°C, respectively. The Tm of non-specific products of W-185 is greatly larger than 79°C.
Chang HW, Cheng CA, Gu DL, Chang CC, Su SH, Wen CH, Chou YC, Chou TC, Yao CT, et al. - BMC Biotechnol. (2008)
Representative gel view and MCA using sex-specific primers of S. c. hoya. PCR products with 185- and 112-bp amplified by (A) CHD-W-F/R and (B) CHD-Z-F/R primers in different PCR-wells...
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getmorefigures.php?pmc=2259332&rFormat=json&query=the&fields=all&favor=none&it=none&sub=none&uniq=1&sp=none&coll=none&req=5
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Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in human lung cancer cells (H1299). Human lung cancer cells (H1299) were treated with media alone (control) and various concentrations of CSE; a) control, b) CSE (1.0%), c) CSE (2.5%), d) CSE (5.0%), e) CSE (7.5%), f) CSE (10%) for 24 hr. The cells were stained with ethidium bromide and acridine orange and observed under fluorescence microscopy. Living cells had normal shaped nuclei with green chromatin. Early apoptotic cells have shrunken green nuclei with chromatin condensation, whereas necrotic or late apoptotic cells had normal/condensed nuclei that were brightly stained with ethidium bromide and appeared red. Percentage of viable (white bars), apoptotic (grey bars) and necrotic/late apoptotic (black bars) determined by counting as described in Materials and Methods. Results are mean of 3 experiments ± SEM. *p < 0.05, and §p < 0.001 compared with control group. L = Live; A = Apoptosis; N = Necrosis.
Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in human lung cancer cells (H1299). Human lung cancer cells (H1299) were treated with media alone (control)...
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