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Groups of 10 BALB/c mice were vaccinated with PAV3-HA at a dose of 108 (⋄), 109 (▿), and 1010 (▵) vp/mouse, AdHu5-HA 108 (♦), 109 (▾), and 1010 (▴), or PBS Control (•).Twenty-eight days post-vaccination, the mice were challenged with 100LD50 of H5N1-H05 virus and monitored for (i) survival and (ii) weight loss and clinical signs of disease. Confirmatory factor analysis. The XPS spectra of CuO nanoparticles annealed at 800°C. The inset shows the Cu 2 p core level binding energy spectra Time-course expression levels of PtrABF in trifoliate orange shoots under different treatments with dehydration (A), low temperature (B), salt (C) and ABA (D). Total RNA extracted from leaves of the treated shoots sampled at the indicated time was reverse-transcribed to synthesize cDNAs, which were used for quantitative real time PCR analysis. For each treatment, the expression level at time point 0 was defined as 1.0, and data represented means ± SE of three replicates. A view of (I) showing the atom-numbering scheme with displacement ellipsoids at the 30% probability level.
A. Change in supplemental salbutamol use before and after exacerbation in patients treated with fluticasone and salmeterol combination or with high-dose fluticasone (with permission from ref 90), B. Change in morning PEF (percent fall from day -14) over the 14 d before and 14 d after an exacerbation in relation to treatment as analyzed from a subgroup of a FACET study (with permission from ref 89). Protection Is Mediated by IL-12 Secreted by Donor DCsTo confirm that IL-12 secreted by the donor DC-mediated protection, groups of six C57BL/6 and IL-12KO mice were infected with nonlethal P. yoelii 17XNL, total CD11c+ DCs were isolated from these infected mice after drug curing, and 1.5 × 107 DCs were transferred to groups naïve mice. After 24 h each mouse was infected with a lethal dose of P. yoelii YM. In all experiments, mice were monitored daily following infection and culled if required, but parasitemia levels were measured every 2–3 d. Error bars represent ± standard error of the mean. This experiment was repeated twice. XRD pattern of the synthesized BaTiO3 product. The inset shows the reflection around 45° 2θ (200)/(002) peak (filled circle measured, thine line fitted, thick line calculated.) Effects of meroditerpenoids on the inhibition of cell proliferation in RBL-2H3 cells using the SRB assay. Values show mean + SE inhibition, as compared to negative control (0.1% DMSO), from three experiments performed in triplicate. * p < 0.05; ** p < 0.005; *** p < 0.0005. Loss of ΔΨm is caspase dependent during apoptosis. (A) Jurkat cells treated with etoposide (40 μM) or Cc-GFP-HeLa cells were treated with actinomycin D (1 μM) in the presence or absence of zVADfmk (100 μM), harvested at the times indicated, stained with TMRE (50 nM), and analyzed by flow cytometry. Low fluorescence indicates a loss of ΔΨm. (B) A representation of A, in which untreated cells (thin lines) are overlaid directly on cells treated with the apoptosis inducer in the presence of zVADfmk (zVAD) (100 μM). In the Cc-GFP-Hela cells, 57% of cells had released cytochrome c–GFP by this point.
Only the 46-61 determinant is highly dependent on Ii coexpression with Ak for its effective presentation. COS cells transfected with cDNA expression constructs encoding either Ak or Ak and the p31 form of mouse Ii were used as APCs with the indicated concentrations of HEL for stimulation of T hybridomas specific for the indicated determinants of HEL. IL-2 production at 24 h is presented as OD obtained using an IL-2–specific capture ELISA. The data represent one of three independent experiments. Partial packing diagram of the unit cell showing key interactions (see text and Table 1) [Macrae et al., 2008]. Only significant H atoms are shown as balls for clarity. Symmetry (i) x - 1/2, 2 - y, z (ii) 1/2 - x, 1 - y, z (iii) x, y - 1, z (iv) 1 - x, 1 - y, 1/2 + z (v) x - 1/2, 1 - y, z. Ring centres shown as red balls are Cg1 (C19–C24), Cg2 (C4–C9) and Cg3 (C13–C18). Diagram of 1370 bp of the murine Bax promoter regionThe Bax promoter region from the DBA/2J and 129B6 mouse lines was isolated, sequenced, and compared. The nucleotide directly upstream of the translation start site is indicated as −1 in the first exon and numbering of the murine Bax promoter is relative to this position. Depicted are the sequences of 30 bp of the 129B6 and DBA/2J promoter regions at positions −499 to −528. A single nucleotide polymorphism (outlined with a dashed box) was found at position −515. The core p53 half-site recognition sequence is Pu Pu Pu C A/T A/T G Py Py Py (where Pu, purine; Py, pyramidine) (El-Deiry et al., 1992). The DBA/2J promoter has a perfect consensus sequence (underlined). Other putative transcription factor binding sites are also shown as previously described (Igata et al., 1999). Temperature over time in Septavacc and control ponies.Mean rectal temperature was monitored from three days pre-challenge (day 85). Average values and SEM are shown. The insert shows number of days each pony was considered pyrexic, i.e. with a temperature exceeding 39.0°C. P-value in insert: **p = 0.01. Non-vaccinated (open symbols) (n = 7) and Septavacc vaccinated (closed symbols) (n = 7). Actions needed to decrease recent tuberculosis transmission in various settings. Decreasing diagnostic delays can potentially eliminate large point source clusters and substantially reduce transmission in both traditional and nontraditional settings. CI, contact investigation.
Single-unit muscle sympathetic nerve activity (MSNA) measured as firing rate, firing probability and probability of multiple firing at baseline (B) and 12 weeks following the diet. *P < 0.05. Demonstration of MPT induction by photoexcited trigger ROS. (A) Cells dual-loaded with TMRM (ΔΨ) and calcein-AM (the latter loaded under conditions that results in a cytosolic distribution initially in excess over that in mitochondria); line-scan imaging at 20 Hz. (B) 10 μM BA partially inhibits the MPT (ΔΨ; a and b) and RIRR (ROS; c and d) versus control. Cells were dual-loaded with TMRM and DCF; line-scan imaging at 2 Hz. (C) 100 μM BA completely inhibits the MPT versus control. Cells are loaded with 125 nM TMRM; line-scan at 2 Hz. Nociceptive behaviour. Tail (A, B) and paw (C, D) withdrawal latency after chronic corticosteroid treatment for 21 (1) and 28 (2) days with CORT and DEX. Both CORT and DEX groups display higher TF latencies after 21 days of treatment (A, B) although this effect is only sustained by DEX group at the end of the experiment (B); note that only DEX induces an increase in hind-paw latency and only after 28 days of treatment (D). (*p < 0.05, **p < 0.01 and ***p < 0.001). Analysis of engraftment in NOD/SCID mice with CD34+CD38−Lin− cells cultured in the absence or presence hJagged-1. (A) Summary of levels of human cell engraftment in the BM of NOD/SCID mice transplanted with CD34+CD38−Lin− cells cultured in the absence (○) or presence (•) of Jagged-1 (10 μg/ml). Cells were cultured for the different periods indicated and transplanted by tail vein injection into NOD/SCID recipients. (i) Mice injected with 1,000–2,500 CD34+CD38−Lin− cells seeded on Day 0. (ii) Mice injected with 500–1,000 cells seeded on day 0. At days 12 and 15, horizontal bars indicate average level of human chimerism achieved in engrafted mice. (B) Representative Southern blot analysis of individual NOD/SCID mice transplanted with four equally divided aliquots of 2,500 CD34+CD38− cells seeded at day 0 and cultured under serum-free conditions for either 12 or 15 d in the presence or absence of hJagged-1. DNA was extracted from recipient murine BM 8 wk after the transplantation, separated on agarose gels, and hybridized with the human chromosome 17–specific α satellite probe. The level of engraftment was determined from the human/mouse DNA controls shown. (C) Multilineage differentiation of human repopulating cells in NOD/SCID mice after ex vivo culture with or without Jagged-1. Cells obtained from the BM of engrafted mice were stained with human specific mAbs and analyzed by flow cytometry. (i) Forward and side scatter properties were used to gate live cells in R1 for analysis. (ii) Isotype control for nonspecific IgG1 staining for PE and FITC fluorescence. Human cells (positive for panleukocyte marker CD45) were gated and analyzed for expression of the following human markers: (iii) pan-B cell markers for cells of the lymphoid lineage CD19 and CD20, (iv) myeloid markers CD15 and CD33, and (v) CD38 and primitive cell markers CD34. Results are shown for representative mice transplanted with control treated and Jagged-1–treated cultures. Culture growth and colony formation in conditions of normal gravity and hypergravity.Identical set of initial random seeds are used for the simulations at normal gravity and hypergravity. (a) Predicted character of movement in normal gravity (G = 1.0) at different time points as shown on the left. The dots correspond to individual cells. (b) Predicted character of movement in hypergravity (G = 1.5) at the different time points. The walls of the virtual Petri dish are shown on the X and the Y. (c) Predicted average cell speed in normal gravity (an empty square) and hypergravity (a full square). X-axis depicts the time rescaled from computer steps to hours. Y-axis shows the average cell speed in links per computer step (hours).
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