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Segregation of primary open-angle glaucoma in a large family from Taxiarchis (Taxi 2). Note that A23 is homozygous for the Thr377Met mutation. A filled-in symbol indicates that the person has been diagnosed with POAG. The number under the symbol indicates the specific code given to the family member who participated in the study. The MYOC genotype is denoted below the code. The arrow indicates the proband for the pedigree. Competing hypotheses for the rooting of angiosperms showing the same underlying angiosperm topology when outgroups are excluded. A. Rooting within monocots (Mono), on the branch between grasses and all other angiosperms (see Fig. 2C, whose BS values are shown here, and also Fig. 2F; also see Goremykin et al. [19]). B. Unrooted network, with arrow showing alternative rootings as in A and C. C. Canonical rooting on the branch between Amborella and the rest of angiosperms (see Fig. 2I, whose BS values are shown here, and also Fig. 2L). We emphasize that 100% BS was obtained for Amborella-sister and for monocot monophyly (compared to 79% and 78% in C) using ML methods that allow for site-to-site rate heterogeneity (e.g., Additional files 1–3). The molecular structure of the title compound, showing the atom-numbering scheme. Displacement ellipsoids are drawn at the 30% probability level. Population analysis of ADC values when the networks were trained using vPFC activity from trials in which the test stimulus was a prototype stimulus only. In each panel, on a neuron-by-neuron basis, two of the three ADC values are compared. In panel (A), the decision-trained-ADC values and relationship-trained-ADC values are compared. In panel (B), the decision-trained-ADC values and test-stimulus-trained-ADC values are compared. In panel (C), the test-stimulus-trained-ADC values and relationship-trained-ADC values are compared. In each panel, the solid line is the line of equal ADC value.
DNA/RNA and PNA and TANA. RO-T1D and aAb+ve unaffected subjects show dramatic decrease in suppression of CD4+CD25low-Tcell proliferation by Tregs.There was statistically significant difference in suppressive potential of CD4+CD25- T cells between control, RO T1D and aAb+ve subjects (Kruskal-Wallis test, p = 0.014, Figure 6A). However, the difference was even more pronounced with CD4+CD25low as responder T cells (Kruskal-Wallis test, p = 0.0007, Figure 6B). Both RO T1D and autoantibody-positive subjects showed significant decrease in suppression of CD4+CD25low T cells (paired t-test, p = 0.0025 and p = 0.008) compared with their respective CD4+CD25- T cells. In addition, there was significant difference in suppression of the two responder T cells in healthy control subjects (p = 0.017). Activation of the mTOR pathway favors senescence in nutlin-3a-arrested cells. Effect of the effluent from canine internal mammary artery on coronary artery endothelium-free ring. Relaxation of the coronary artery endothelium-free ring induced by the effluent released from canine internal mammary artery stimulated by intraluminal and extraluminal perfusion of acetylcholine (10-5 M) before (control) and after intraluminal and extraluminal atropine (10-5 M). Values represent mean ± SEM; n = 6. Relaxation magnitude is expressed as % of initial tonus. * p < 0.05. Increase in weight of virgin rats during the administration of THAT before mating.
Genes involved in the signalling cascade for detection of AI-2 in Vibrio. Abbreviations: OM outer membrane; IM inner membrane; H histidine; D aspartate; HTH helix turn helix motif; sRNA small regulatory RNAs; Hfq chaperone protein. Numbers indicate analyzed organisms that have an orthologous gene for that protein using a reciprocal best hit search strategy, numbers in brackets indicate the number of analysed organisms having a similar gene based on standard blast search. XRD pattern of 5.2-nm CdS NCs synthesized at 68 s (OA: 51.2 vol %) Sphingolipid composition of lipid rafts and soluble domains in cultured skin fibroblasts. Lipid rafts were isolated from cultured skin fibroblasts (6 mg of total cell protein) and the lipids present in each of the 12 fractions were extracted and analysed by mass spectrometry as described previously [50]. Individual species of ceramide (Cer, closed squares), glucosylceramide (GC, open diamonds), lactosylceramide (LC, closed triangles) and trihexosylceramide (THC, crosses) were summed and shown. Results are expressed as pmol lipid per mg of total protein. Lipid rafts are localised to fractions 3 and 4. Species studied, with estimated branch length, in average changes per synonymous site (dS), from reference [28]. Differences in the percentage of dendritic cells. Fresh peripheral blood samples were evaluated by flow cytometry for the presence of Lin-HLA-DR+ dendritic cells at baseline, following SSG monotherapy (C1D8) and following SSG + IFN-α combination therapy (C2D12). (A) Following SSG monotherapy, there was a transient decrease in the percentage of CD11c+CD123- mDC within the Lin-HLA-DR+ population followed by a significant increase with the addition of IFN-α 2b. (B) The percentage of CD11c-CD123+ pDC decrease within the Lin-HLA-DR+ DC population following SSG monotherapy.
Metabolic pathway for GDP-fucose synthesis in Saccharomyces cerevisiae [17]. Enzymes – 1: GDP-mannose-4,6-dehydratase (Gmd), 2: GDP-4-keto-6-deoxy-mannose-3,5-epimerase (GmeR), 3: 4-reductase (GmeR), 4: GDP-fucose pyrophosphorylase, 5: fucose kinase. Experimental scheme. Age- and sex-matched mice were immunized intraperitoneally with 50 μg/mouse of DNP-KLH in CFA. Sera were collected at 1 wk after the primary immunization. The immunized mice were boosted with 50 μg of KLH-DNP in IFA at 2 wk after the primary immunization, and the sera were collected 2 wk after the second immunization. At 1 mo after the second immunization, mice were killed and the spleen cells were harvested for ELISA spot assay. Hormone profiles.Plasma concentrations of A) corticosterone, B) leptin and C) adiponectin as a function of age in GK and WKY animals. Data represent means and error bars 1 SD of the mean. Closed circles = GK; open circles = WKY). * = P<0.05; ** = P<0.001. Retrosynthetic Analysis of Scytonemin Phenotype and cytokine production by activated CD4−/− and CD4+/+ AND T cells. (A) Phenotype of purified CD4+/+ and CD4−/− AND T cells. AND T cells on CD4+/+ or CD4−/− background were isolated using negative selection as described in Materials and Methods and then analyzed for Vα11 (AND TCR transgene bears Vα11 and Vβ3) and CD4 expression. Wt, wild-type; KO, knockout. (B) Activation marker expression by stimulated and naive AND T cells. CD4+/+ and CD4−/− AND T cells were stimulated with MHC/peptide beads for 6 d in 24-well plates and then assayed for CD44, CD45RB, and CD62L expression. Right panel shows CD62L expression on unstimulated CD4+/+ and CD4−/− cells. Shaded histograms represent unstained controls. (C) Cytokines production by activated CD4−/− and CD4+/+ T cells. T cells were collected from day 6 cultures, washed, and stimulated overnight with various doses of MHC/peptide beads and culture supernatants were assayed for IFN-γ and IL-2 by ELISA.
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