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Rate of cell-cell movement of TEV-GUS in inoculated leaves of B149 and C24.Leaves from plants inoculated with TEV-GUS were harvested at time points indicated and infiltrated with the colorimetric substrate X-gluc. Foci diameter were measured microscopically at time points indicated and represented as mean (±SD). Data at 24 h represent mean of 17 foci, other points represent mean of 39 foci. Histograms of step sizes. (A) For each granule that could be tracked for at least five consecutive frames (997 granules from 12 cells), <(Î配)2> was computed from an average of each interframe step size for that granule, and then allocated into bins of 100.1 width on a log scale of <(Î配)2>. Note that the absolute magnitudes shown on the abscissa can also be read as diffusion coefficients in view of , where the interframe time T = 0.5 s. (B) For all granules appearing in at least two consecutive frames (12 cells, 21,530 individual granule motions), all the individual (Î配)2 values were pooled (regardless of their granule of origin), and then allocated into bins of 100.1 width on a log scale of (Î配)2. Both of these histograms show that there are no distinct groups of slow and fast granules, but rather a broad continuum. Relationship between the average BMD of lumbar spine bones and bilateral femoral necks. The average BMD of femoral neck showed a good correlation with lumbar vertebra (Average BMD of lumbar spine bones = 0.718 à Average BMD of bilateral femoral necks +336.46;r = 0.959; p < 0.0001). Correlation between FWHM measured in the PET images using an activity profile across the centre of the syringes and the actual diameter of the static arterial phantom syringes. Lines represent the fits: for the 18F-FDG brain imaging protocol with 2-mm voxels on the Gemini TF PET/CT scanner (dotted line, circles), and for 11C-acetate (solid line, squares) and 18F-FTHA with a whole-body protocol and 4-mm voxels on the Gemini GXL PET/CT scanner (dashed line, crosses) H2O2 induced a dose-dependent viability loss in PC12 cells. *P < .01 versus control (0âμM).
(A) Development of H-Y antigenâ都pecific T cells in male or female thymectomized mice.  C57BL/6 mice (H-2b) with or without adult  thymectomy (ATXâ) were lethally irradiated (11 Gy)  and injected intravenously with 5 à 106 T cellâ depleted bone marrow cells from female H-Y transgenic mice (H-2b). 7 wk after bone marrow transfer,  development of T cells with transgenic TCR α  chain (T3.70+) in the spleen and liver was examined  by flow cytometric analysis. Four mice are examined, and representative data are shown. The same  experiments were repeated, and similar results were  obtained. (B) Expression levels of CD8 on T3.70+  cells developed in the bone marrow chimera mice.  Expression of CD8 on T3.70+ cells in the spleen  (ââ映illed histograms) and liver (open histograms) were analyzed by overlaying histograms. Swelling patterns of matrix tablets with change in SB concentration (mean ± SD, n = 3) Schematic representation of PED/PEA-15 effect on cytoskeletal organization and cell motility. Many growth factors, extracellular matrix proteins, and other extracellular signals induce ERK1/2 activation and translocation from cytosol (Cyt-ERK1/2) to nucleus (Nuc-ERK1/2), as well as RhoA activity and cytoskeletal rearrangements allowing cellular motility. When PED/PEA-15 is hyper-expressed, it binds ERK1/2 and prevents nuclear translocation, thereby causing accumulation into the cytosol. This is paralleled by a decrease of RhoA activity, which most likely affects the formation of FAPs and stress fibers. In cells overexpressing PED/PEA-15, biochemical and morphological changes are also accompanied by an overall reduced cellular motility. Scheme of cryoablation system. The steerable catheter and the console are connected by: 1) a coaxial cable, used both to deliver fluid nitrous oxide to the catheter and to remove separately the gas from the catheter; 2) electrical cable, which is connected both to the conventional recording system for electrograms (EGMs) analysis and storage and to the console for reading of the tip temperature. A tank of fluid nitrous oxide is located inside the console; the gas removed from the catheter to the console is evacuated through a scavenging hose into the vacuum line of the electrophysiology laboratory. The system has several sensors to avoid inadvertent leaks of nitrous oxide into the patient body and to check connections of the different cables to the console. The relation between the cholesterol content of red cells and phase angle determined by bioelectrical impedance analysis for SCD subjects and controls; â酪, SCD subjects; â雷, controls; p = 0.003, r2 = 0.04.
Inhibitory effect of KIN-841 on proliferation of 3LL cells under hypoxia. Cells were treated for 48â栄 with KIN-841 at the various concentrations indicated under normoxia or hypoxia. aP<0.05 vs controls; bP<0.01 vs controls; cP<0.05 vs normoxia. Pressure-dependence of expiratory flow. Mean flow-volume curves from ovalbumin-sensitized and sham-challenged mice (A) and ovalbumin-sensitized and challenged mice (B) at varying negative pressures. Values are mean ± standard deviation from groups of 7-9 mice (1 determination per animal at each negative pressure). Cumulative number of coding mutations, cis-regulatory mutations and other types of mutations (gene amplification, gene loss, etc.) that have been identified over time as responsible for phenotypic evolution. Results are from data in Appendix 1. Note that the slope for cis-regulatory mutations has increased in recent years. The current discovery rate of cis-regulatory mutations approximately equals the discovery rate of coding mutations. If this reflects the long-term trend, then we expect ultimately to observe approximately equal numbers of cis-regulatory and coding mutations. Carriers of APOC3 variant alleles (-482T, -455C or both) were compared with homozygous for the wild-type alleles at both SNPs (-482C and -455T alleles) for glucose and insulin measurements during oral glucose tolerance test (OGTT). The study cohort underwent OGTT (75 g) test and glucose and insulin were measured baseline (0) and at 30, 60, 90 and 120 min after the administration of glucose. (A) Median glucose and (B) insulin in individuals stratified by the genotypes. No statistical differences were found in either insulin or glucose at different time points in the two groups. P-values were calculated by linear regression (all P-values = NS), including age, gender and body mass index (BMI) as covariates in the model.
Activation of CPP32-like proteases in normal and resistant  U937 variants. (A) Normal U937 cells, TNF-selected variants (U9-TR),  and NAD-depleted variants (U9-NAD) were exposed to UV light 0.06  J/cm2, incubated for the indicated time, and cytosol extracts were assayed  for proteolytic activity on the DEVD-pNa substrate. (B) U937 and the  variants were incubated for 90 min after exposure to UV light, and the  morphologically apoptotic cells were enumerated microscopically. Effects of Ca2+ withdrawal on twitches in MG29-deficient skeletal muscle. Twitch tension at 5, 10, and 20 min after Ca2+ removal from the extracellular solution was measured in diaphragm muscles from 8â9-wk-old mice. Each value normalized to tension under normal conditions represents the mean ± SD. (n = 7â9 from six mice). Significant differences between the groups were observed by t test (asterisks indicate P < 0.01). Overall survival (ITT analysis). Piceatannol pretreatment inhibits NK cellâ mediated cytotoxicity. NK cells  were treated for 15 min at 37°C  with the indicated concentration of piceatannol, washed, and  then incubated for 4 h with either 51Cr-labeled K562 cells or  51Cr-labeled P815 cells coated  with 0.15 μg/ml of the anti-FcR  mAb 3G8. Data are expressed as percentage of inhibition of lytic U/106  cells calculated from NK cells incubated in vehicle alone. Comparison of the effects of kainate and carbachol. Simultaneous recording of Em and [Mg2+]i. At an [Mg2+]o of 1 mM, the application of 500 μM carbachol and of 100 μM kainate elicited membrane depolarizations of similar size. Application of kainate but not of carbachol caused an additional increase in [Mg2+]i. An increase in [Mg2+]o to 10 mM caused an increase in [Mg2+]i, which was not affected by a further application of carbachol but greatly enhanced by the application of kainate.
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