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Mentions: PMMA-embedded lumbar vertebrae (L3) were cut to give 3-mm midshaft cross-sections for 3D data and image acquisition using a desktop μCT system (μCT-40; Scanco Medical, Bassersdorf, Switzerland). Micro-CT scans were performed on the entire 3-mm specimen to give 100 successive 30-μm slices. Scanning was performed with the following parameters: 21-μm isotropic voxel size, 55-keV voltage, 109-mA current, 200-ms integration time, and 4,000 projections. Three-dimensional information was obtained by stacking the measured slices on top of each other. After digital removal of the surrounding cortical bone, the entire spongiosa was used for subsequent morphometric analysis. A representative 3D visualization of a complete measurement is shown in Fig. 2. Bone volume fraction (bone volume/total volume (BV/TV)), trabecular thickness (Tb.Th), number (Tb.N), and separation (Tb.Sp) were also evaluated. The connectivity density (Conn.D) was calculated using the Euler method .Fig. 2
Microarchitecture and Nanomechanical Properties of Trabecular Bone After Strontium Administration in Osteoporotic Goats
Bottom Line: However, the effect of Sr treatment on bone microarchitecture and bone nanomechanical properties remains unclear.Sr administration significantly increased trabecular bone volume fraction, trabecular thickness, and double-labeled new bone area.Sr administration, however, did not significantly change the nanomechanical properties of trabecular bone (elastic modulus and hardness).
Affiliation: Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China.
Strontium (Sr) ralenate is a new agent used for the prevention and treatment of osteoporosis. As a bone-seeking element, 98% of Sr is deposited in the bone and teeth after oral ingestion. However, the effect of Sr treatment on bone microarchitecture and bone nanomechanical properties remains unclear. In this study, 18 osteoporotic goats were divided into four groups according to the treatment regimen: control, calcium alone (Ca), calcium and Sr at 24 mg/kg (Ca + 24Sr), and calcium and Sr at 40 mg/kg (Ca + 40Sr). The effects of Sr administration on bone microarchitecture and nanomechanical properties of trabecular bones were analyzed with micro-CT and nanoindentation test, respectively. Serum Sr levels increased six- and tenfold in the Ca + 24Sr and Ca + 40Sr groups, respectively. Similarly, Sr in the bone increased four- and sixfold in these two groups. Sr administration significantly increased trabecular bone volume fraction, trabecular thickness, and double-labeled new bone area. Sr administration, however, did not significantly change the nanomechanical properties of trabecular bone (elastic modulus and hardness). The data suggested that Sr administration increased trabecular bone volume and improved the microarchitecture while maintaining the intrinsic tissue properties in the osteoporotic goat model.
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