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CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

Bottom Line: Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT

Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

TEM images of Cdte QDs: (A) the low magnification images Cdte QDs, (a) HRTEM image of an individual nanocrystal of Cdte QDs. (B) Size of Cdte QDs suspended in cell culture medium was analyzed by dynamic light scattering. (C) Emission spectrum of Cdte QDs, excitation wavelength at 330 nm.
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Figure 1: TEM images of Cdte QDs: (A) the low magnification images Cdte QDs, (a) HRTEM image of an individual nanocrystal of Cdte QDs. (B) Size of Cdte QDs suspended in cell culture medium was analyzed by dynamic light scattering. (C) Emission spectrum of Cdte QDs, excitation wavelength at 330 nm.

Mentions: The water-soluble Cdte QDs capped with negatively charged 3-mercaptopropionic acid were prepared according to the procedure as reported previously. Our TEM study illustrates that the average size of Cdte QDs was about 4 nm, as shown in Figure 1A, and an HRTEM individual nanocrystal of Cdte QDs (Figure 1A a, HRTEM). The Cdte QDs in cell culture medium were about 5 nm, as characterized with dynamic light scattering (Figure 1B). The typical fluorescence spectrum of the Cdte QDs was shown in Figure 1C.

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CdTe quantum dots with daunorubicin induce apoptosis of multidrug-resistant human hepatoma HepG2/ADM cells: in vitro and in vivo evaluation

Zhang G, Shi L, Selke M, Wang X - Nanoscale Res Lett (2011)

TEM images of Cdte QDs: (A) the low magnification images Cdte QDs, (a) HRTEM image of an individual nanocrystal of Cdte QDs. (B) Size of Cdte QDs suspended in cell culture medium was analyzed by dynamic light scattering. (C) Emission spectrum of Cdte QDs, excitation wavelength at 330 nm.
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Figure 1: TEM images of Cdte QDs: (A) the low magnification images Cdte QDs, (a) HRTEM image of an individual nanocrystal of Cdte QDs. (B) Size of Cdte QDs suspended in cell culture medium was analyzed by dynamic light scattering. (C) Emission spectrum of Cdte QDs, excitation wavelength at 330 nm.
Mentions: The water-soluble Cdte QDs capped with negatively charged 3-mercaptopropionic acid were prepared according to the procedure as reported previously. Our TEM study illustrates that the average size of Cdte QDs was about 4 nm, as shown in Figure 1A, and an HRTEM individual nanocrystal of Cdte QDs (Figure 1A a, HRTEM). The Cdte QDs in cell culture medium were about 5 nm, as characterized with dynamic light scattering (Figure 1B). The typical fluorescence spectrum of the Cdte QDs was shown in Figure 1C.

Bottom Line: Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery.We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells.Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

Affiliation: State Key Lab of Bioelectronics (Chien-Shiung Wu Lab), Department of Biological Science and Medical Engineering Southeast University, Nanjing, 210096, PR China. xuewang@seu.edu.cn.

ABSTRACT

Background: Cadmium telluride quantum dots (Cdte QDs) have received significant attention in biomedical research because of their potential in disease diagnosis and drug delivery. In this study, we have investigated the interaction mechanism and synergistic effect of 3-mercaptopropionic acid-capped Cdte QDs with the anti-cancer drug daunorubicin (DNR) on the induction of apoptosis using drug-resistant human hepatoma HepG2/ADM cells. Electrochemical assay revealed that Cdte QDs readily facilitated the uptake of the DNR into HepG2/ADM cells. Apoptotic staining, DNA fragmentation, and flow cytometry analysis further demonstrated that compared with Cdte QDs or DNR treatment alone, the apoptosis rate increased after the treatment of Cdte QDs together with DNR in HepG2/ADM cells. We observed that Cdte QDs treatment could reduce the effect of P-glycoprotein while the treatment of Cdte QDs together with DNR can clearly activate apoptosis-related caspases protein expression in HepG2/ADM cells. Moreover, our in vivo study indicated that the treatment of Cdte QDs together with DNR effectively inhibited the human hepatoma HepG2/ADM nude mice tumor growth. The increased cell apoptosis rate was closely correlated with the enhanced inhibition of tumor growth in the studied animals. Thus, Cdte QDs combined with DNR may serve as a possible alternative for targeted therapeutic approaches for some cancer treatments.

View Similar Images In: Results  - Collection
View Article: Pubmed Central - HTML -  PubMed
Show All Figures - Show MeSH
getmorefigures.php?pmc=3211514&rFormat=json&query=null&req=5