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Germ tube of candida seen under a light microscope
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Figure 2: Germ tube of candida seen under a light microscope

Mentions: Method of identification: Swabs were inoculated immediately on the Sabouraud's slope. The growth was subcultured on a new slope of Sabouraud's agar and the pure colony was subjected to the GT test and carbohydrate fermentation test [Figure 1,Figure 2].

Candida and calcofluor white: Study in precancer and cancer

Kumar RS, Ganvir S, Hazarey V - J Oral Maxillofac Pathol (2009)

Bottom Line: A highly significant association of Candida was seen more often in cancer than in precancer.In precancer it was 48.88% in smears and 40% in tissue sections, whereas, in cancer 60% in smears and 55.55% in histopathology.Among the various diagnostic tools used in the present study, the use of CFW is seen to be a simple, effective, rapid, and reliable method, both in cytopathology and histopathology.

Affiliation: Department of Oral and Maxillofacial Pathology and Microbiology, Kamineni Institute of Dental Sciences, Narketpalli - 508254, Andhra Pradesh, India.

ABSTRACT

Background: The interest in oral candidosis has waxed and waned from the period of Hippocrates. The acquired immune deficiency syndrome (AIDS) epidemic has certainly bolstered these figures on oral candidosis, with diabetes and oral cancer being no exception. A need for rapid detection of Candida is made possible by the use of Calcofluor - White (CFW) stain when examined under a fluorescence microscope. The present study was aimed at assessing the efficacy of CFW is compared to Gram stain and periodic acid Schiff (PAS) in detection of Candida in oral precancer and cancer.

Materials and methods: The study group consisted of patients with precancer (n=45), cancer (n=45), and control group (n=45). Presence of Candida was confirmed by culture inoculation along with a germ tube and carbohydrate fermentation test. The cytopathological smears were analyzed by papanicolaou - CFW and Gram staining, whereas, tissue sections were stained by PAS and CFW staining.

Results: Candida albicans was the predominant species identified. A highly significant association of Candida was seen more often in cancer than in precancer. Both in cytology and histopathology Candida detection by CFW was higher. In precancer it was 48.88% in smears and 40% in tissue sections, whereas, in cancer 60% in smears and 55.55% in histopathology.

Conclusion: Among the various diagnostic tools used in the present study, the use of CFW is seen to be a simple, effective, rapid, and reliable method, both in cytopathology and histopathology.

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