pntd-0001223-g003: Output of the DPnII-PCR-RFLP with lanes 1, 6, and 10 as size markers (100-bp ladder), lanes 2, 3, 4, 5, and 7 as sensitive profiles (one band), lane 8 as resistant profile (two bands), and lane 9 as mixed profile (three bands).

Mentions: The DPnII-PCR-RFLP profiles of the 101 amplified T. congolense samples were distributed as follows: 92% were resistant, 2% sensitive, and 6% presented a mixed profile (Figure 3). Even if due to host-related factors not all animals with those infections would fail to respond to treatment, such a high prevalence of DA resistance is more than worrying. As proposed by Clausen et al. [7], in such circumstances an integrated approach should be used based on (i) rational drug use information given to farmers, (ii) training farmers and paravets in integrated trypanosomiasis control, and (iii) training animal care providers to a more specific diagnosis of trypanosomiasis to avoid treatments inappropriate for the clinical signs.

Vitouley HS, Mungube EO, Allegye-Cudjoe E, Diall O, Bocoum Z, Diarra B, Randolph TF, Bauer B, Clausen PH, Geysen D, Sidibe I, Bengaly Z, Van den Bossche P, Delespaux V - PLoS Negl Trop Dis (2011)

Affiliation: Centre International de Recherche-Développement sur l'Elevage en zone Subhumide, Bobo Dioulasso, Burkina Faso.