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Genetic Diversity of the Planthopper, Sogatella furcifera in the Greater Mekong Subregion Detected by Inter-Simple Sequence Repeats (ISSR) Markers

Liu JN, Gui FR, Li ZY - J. Insect Sci. (2010)

Bottom Line: According to the hierarchical analysis of molecular variance (AMOVA), the genetic variation among geographic regions (79.84%) was higher than that of among populations within region (20.16%), and the FST value was 0.72, indicating a high level of genetic differentiation.Neighbor-Joining cluster analysis of the 47 populations showed two major clusters, one consisting of mostly southwestern Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central of Yunnan Province plus Vietnam and Laos populations.No significant positive correlation was observed between genetic and geographic distances by Mantel test (r = 0.2230, p = 0.8448), indicating the role of geographic isolation did not shape the genetic structure of the sampled S. furcifera populations.

Affiliation: Key Laboratory for Agricultural Biodiversity and Pest Management of Ministry of Education, Plant Protection College, Yunnan Agricultural University, Kunming 650201, China.

ABSTRACT

The white backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae), is a serious pest of rice in Asia. In the present study, inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 47 populations sampled from 14 prefectures of the Greater Mekong Subregion. A total of 14 selected primers yielded 121 bright and discernible bands, with an average of 8.6 bands per primer. According to the hierarchical analysis of molecular variance (AMOVA), the genetic variation among geographic regions (79.84%) was higher than that of among populations within region (20.16%), and the FST value was 0.72, indicating a high level of genetic differentiation. Neighbor-Joining cluster analysis of the 47 populations showed two major clusters, one consisting of mostly southwestern Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central of Yunnan Province plus Vietnam and Laos populations. No significant positive correlation was observed between genetic and geographic distances by Mantel test (r = 0.2230, p = 0.8448), indicating the role of geographic isolation did not shape the genetic structure of the sampled S. furcifera populations. This paper provides useful data for understanding and speculating the migration of S. furcifera and reveals available information to develop sustainable strategies for manage this long-range migratory pest.

Neighbour-joining cluster analysis of the 47 populations of Sogatella furcifera generated from the Dice distance matrix. Only bootstrap values over 50% are shown. The numbers refer to Appendix — available online in supplementary material. High quality figures are available online.
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f03: Neighbour-joining cluster analysis of the 47 populations of Sogatella furcifera generated from the Dice distance matrix. Only bootstrap values over 50% are shown. The numbers refer to Appendix — available online in supplementary material. High quality figures are available online.

Mentions: The dendrogram generated from the NJ cluster analysis showed the genetic relationship among 47 S. furcifera populations; the data are shown in Figure 3. The populations collected from similar geographic regions generally grouped in the same cluster or nearby clusters. Two major clusters, one consisting of mostly southwestern populations of Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central populations of Yunnan Province (including all the Red River Delta populations), plus Vietnam and Laos populations, were visible in NJ cluster analysis. The UPGMA tree showed a similar pattern of clustering with the NJ tree, and the results of the Mantel test indicated a highly significant cophenetic correlation (r = 0.7748, p = 0.0001) between the NJ tree and the UPGMA tree.

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Genetic Diversity of the Planthopper, Sogatella furcifera in the Greater Mekong Subregion Detected by Inter-Simple Sequence Repeats (ISSR) Markers

Liu JN, Gui FR, Li ZY - J. Insect Sci. (2010)

Neighbour-joining cluster analysis of the 47 populations of Sogatella furcifera generated from the Dice distance matrix. Only bootstrap values over 50% are shown. The numbers refer to Appendix — available online in supplementary material. High quality figures are available online.
© Copyright Policy
f03: Neighbour-joining cluster analysis of the 47 populations of Sogatella furcifera generated from the Dice distance matrix. Only bootstrap values over 50% are shown. The numbers refer to Appendix — available online in supplementary material. High quality figures are available online.
Mentions: The dendrogram generated from the NJ cluster analysis showed the genetic relationship among 47 S. furcifera populations; the data are shown in Figure 3. The populations collected from similar geographic regions generally grouped in the same cluster or nearby clusters. Two major clusters, one consisting of mostly southwestern populations of Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central populations of Yunnan Province (including all the Red River Delta populations), plus Vietnam and Laos populations, were visible in NJ cluster analysis. The UPGMA tree showed a similar pattern of clustering with the NJ tree, and the results of the Mantel test indicated a highly significant cophenetic correlation (r = 0.7748, p = 0.0001) between the NJ tree and the UPGMA tree.

Bottom Line: According to the hierarchical analysis of molecular variance (AMOVA), the genetic variation among geographic regions (79.84%) was higher than that of among populations within region (20.16%), and the FST value was 0.72, indicating a high level of genetic differentiation.Neighbor-Joining cluster analysis of the 47 populations showed two major clusters, one consisting of mostly southwestern Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central of Yunnan Province plus Vietnam and Laos populations.No significant positive correlation was observed between genetic and geographic distances by Mantel test (r = 0.2230, p = 0.8448), indicating the role of geographic isolation did not shape the genetic structure of the sampled S. furcifera populations.

Affiliation: Key Laboratory for Agricultural Biodiversity and Pest Management of Ministry of Education, Plant Protection College, Yunnan Agricultural University, Kunming 650201, China.

ABSTRACT

Background: The white backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae), is a serious pest of rice in Asia. In the present study, inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and differentiation of 47 populations sampled from 14 prefectures of the Greater Mekong Subregion. A total of 14 selected primers yielded 121 bright and discernible bands, with an average of 8.6 bands per primer. According to the hierarchical analysis of molecular variance (AMOVA), the genetic variation among geographic regions (79.84%) was higher than that of among populations within region (20.16%), and the FST value was 0.72, indicating a high level of genetic differentiation. Neighbor-Joining cluster analysis of the 47 populations showed two major clusters, one consisting of mostly southwestern Yunnan Province and Myanmar populations; and the other one consisting of southeastern and central of Yunnan Province plus Vietnam and Laos populations. No significant positive correlation was observed between genetic and geographic distances by Mantel test (r = 0.2230, p = 0.8448), indicating the role of geographic isolation did not shape the genetic structure of the sampled S. furcifera populations. This paper provides useful data for understanding and speculating the migration of S. furcifera and reveals available information to develop sustainable strategies for manage this long-range migratory pest.

View Similar Images In: Results  - Collection
View Article: Pubmed Central -  PubMed
Show All Figures - Show MeSH
getmorefigures.php?pmc=3014807&rFormat=json&query=null&req=5