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Mentions: Isolates of H. pylori were identified from culture taken from the gastric cardia, body and antrum of the patients from India and the USA (Table 3). Among total 31 isolates from 13 patients, all 19 isolates from 8 Indian patients had a 650 bp band, and the 11 isolates from 5 patients from the USA had a 650 bp bands and one isolate had a 550 bp band. In two isolates from one patient (147 strain), the antral isolates (147 antral strain) had a 550 bp band and the cardia isolates (147 cardia strain) had a 650 bp bands (Fig. 2). To confirm whether the two strains with differences in the 3'RR were the same, RAPD-PCR was performed for fingerprinting. The RAPD-PCR showed an identical profile in the antral and cardia isolates (Fig. 3). These findings indicated that they were clonal variants of one another. The study of single colonies from the antrum and cardia, with the primer cag3/4 set, showed that the three antral isolates had a 550 bp band, whereas the five cardia isolates had a 650 bp band (Fig. 4).
Examination of Geographical, Clinical and Intrahost Variations in the 3' Repeat Region of CagA Gene in Helicobacter pylori
Bottom Line: The frequency of polymorphisms in the 3'RR did not differ when compared to the clinical manifestations (P=0.868).The length of 3'RR did not differ by location of colonization.In conclusion, the 3'RR variation of cagA gene is not associated with the geographical and clinical characteristics of the patients studied.
Affiliation: Department of Gastroenterology, Kyungpook National University, Daegu, Korea.
The size variation of the cytoxin-associated protein (cagA), which is dependent on the 3' repeat region (3'RR) of the cagA gene, is known to play a crucial role in the pathogenesis of Helicobacter pylori infection. The present study evaluated the relationship between the 3'RR variation and the geographic distribution, clinical manifestations, and locations of colonization in the stomach. We evaluated the 3'RR of H. pylori isolates from 78 patients with gastric cancer, peptic ulcer, and non-ulcer dyspepsia from Japan, Hong Kong, India, and the United States and assessed the variations of 3'RR according to the geographical and clinical characteristics. Sixty eight (87.2%) patients had the same 650 bp band without geographical differences. The frequency of polymorphisms in the 3'RR did not differ when compared to the clinical manifestations (P=0.868). The length of 3'RR did not differ by location of colonization. In conclusion, the 3'RR variation of cagA gene is not associated with the geographical and clinical characteristics of the patients studied.
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