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Anti-inflammatory effects of Radix Gentianae Macrophyllae (Qinjiao), Rhizoma Coptidis (Huanglian) and Citri Unshiu Pericarpium (Wenzhou migan) in animal models

Kim KS, Rhee HI, Park EK, Jung K, Jeon HJ, Kim JH, Yoo H, Han CK, Cho YB, Ryu CJ, Yang HI, Yoo MC - Chin Med (2008)

Bottom Line: KHU14 exhibited anti-inflammatory effects in animal models of acute and chronic inflammation.KHU14 inhibited the production of NO and PGE2 in LPS/IFN-gamma-stimulated peritoneal macrophages, and reduced edema and the amount of infiltrated cells in animal models.KHU14 exhibited anti-inflammatory effects as demonstrated in typical immunological tests for anti-inflammation in vitro and in vivo.

Affiliation: East-West Bone and Joint Research Center, Kyung Hee University Neo Medical Hospital, Seoul, South Korea. labrea46@yahoo.co.kr

ABSTRACT

Background: KHU14, an ethanolic extract of Radix Gentianae Macrophyllae (Qinjiao), Rhizoma Coptidis (Huanglian) and Citri Unshiu Pericarpium (Wenzhou migan) was tested for its anti-inflammatory effects.

Methods: Three out of 20 herbs were found to have anti-inflammatory effects. The formulation of these herbs, i.e. KHU14 was tested for croton oil-induced ear edema, carrageenan-induced paw edema, acetic acid-induced capillary permeability, cotton pellet and delayed type hypersensitivity.

Results: KHU14 exhibited anti-inflammatory effects in animal models of acute and chronic inflammation. The anti-inflammatory activity of KHU14 observed was comparable to that of celecoxib. KHU14 inhibited the production of NO and PGE2 in LPS/IFN-gamma-stimulated peritoneal macrophages, and reduced edema and the amount of infiltrated cells in animal models.

Conclusion: KHU14 exhibited anti-inflammatory effects as demonstrated in typical immunological tests for anti-inflammation in vitro and in vivo.

Anti-inflammatory effects of KHU14 on a mouse model of chronic inflammation. (A) Cotton pellet test (n = 6). (B) DTH (n = 12). The mice were orally fed with KHU14 (400 mg/kg of body weight) and celecoxib (100 mg/kg of body weight) for cotton pellet test and dexamethasone (1 mg/kg of body weight) for DTH. The control group received 0.5% CMC orally. NS: not significant.
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Figure 4: Anti-inflammatory effects of KHU14 on a mouse model of chronic inflammation. (A) Cotton pellet test (n = 6). (B) DTH (n = 12). The mice were orally fed with KHU14 (400 mg/kg of body weight) and celecoxib (100 mg/kg of body weight) for cotton pellet test and dexamethasone (1 mg/kg of body weight) for DTH. The control group received 0.5% CMC orally. NS: not significant.

Mentions: In the cotton pellet test, the dry cotton in CMC, celecoxib, and KHU14 groups weighted 74.7 mg, 63.3 mg, and 55.3 mg respectively (Figure 4A). KHU14 inhibited the infiltration of immune cells by 26% with statistical significance. In the oxazolone-induced DTH model, the right ear thickness of the control group increased 72.1%, while it increased 59.0% and 58.6% in the dexamethasone group and KHU14 group respectively. The results indicate that dexamethsone and KHU14 reduced ear swelling by 18% and 19% respectively (Figure 4B).

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Anti-inflammatory effects of Radix Gentianae Macrophyllae (Qinjiao), Rhizoma Coptidis (Huanglian) and Citri Unshiu Pericarpium (Wenzhou migan) in animal models

Kim KS, Rhee HI, Park EK, Jung K, Jeon HJ, Kim JH, Yoo H, Han CK, Cho YB, Ryu CJ, Yang HI, Yoo MC - Chin Med (2008)

Anti-inflammatory effects of KHU14 on a mouse model of chronic inflammation. (A) Cotton pellet test (n = 6). (B) DTH (n = 12). The mice were orally fed with KHU14 (400 mg/kg of body weight) and celecoxib (100 mg/kg of body weight) for cotton pellet test and dexamethasone (1 mg/kg of body weight) for DTH. The control group received 0.5% CMC orally. NS: not significant.
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Figure 4: Anti-inflammatory effects of KHU14 on a mouse model of chronic inflammation. (A) Cotton pellet test (n = 6). (B) DTH (n = 12). The mice were orally fed with KHU14 (400 mg/kg of body weight) and celecoxib (100 mg/kg of body weight) for cotton pellet test and dexamethasone (1 mg/kg of body weight) for DTH. The control group received 0.5% CMC orally. NS: not significant.
Mentions: In the cotton pellet test, the dry cotton in CMC, celecoxib, and KHU14 groups weighted 74.7 mg, 63.3 mg, and 55.3 mg respectively (Figure 4A). KHU14 inhibited the infiltration of immune cells by 26% with statistical significance. In the oxazolone-induced DTH model, the right ear thickness of the control group increased 72.1%, while it increased 59.0% and 58.6% in the dexamethasone group and KHU14 group respectively. The results indicate that dexamethsone and KHU14 reduced ear swelling by 18% and 19% respectively (Figure 4B).

Bottom Line: KHU14 exhibited anti-inflammatory effects in animal models of acute and chronic inflammation.KHU14 inhibited the production of NO and PGE2 in LPS/IFN-gamma-stimulated peritoneal macrophages, and reduced edema and the amount of infiltrated cells in animal models.KHU14 exhibited anti-inflammatory effects as demonstrated in typical immunological tests for anti-inflammation in vitro and in vivo.

Affiliation: East-West Bone and Joint Research Center, Kyung Hee University Neo Medical Hospital, Seoul, South Korea. labrea46@yahoo.co.kr

ABSTRACT

Background:

Background: KHU14, an ethanolic extract of Radix Gentianae Macrophyllae (Qinjiao), Rhizoma Coptidis (Huanglian) and Citri Unshiu Pericarpium (Wenzhou migan) was tested for its anti-inflammatory effects.

Methods: Three out of 20 herbs were found to have anti-inflammatory effects. The formulation of these herbs, i.e. KHU14 was tested for croton oil-induced ear edema, carrageenan-induced paw edema, acetic acid-induced capillary permeability, cotton pellet and delayed type hypersensitivity.

Results: KHU14 exhibited anti-inflammatory effects in animal models of acute and chronic inflammation. The anti-inflammatory activity of KHU14 observed was comparable to that of celecoxib. KHU14 inhibited the production of NO and PGE2 in LPS/IFN-gamma-stimulated peritoneal macrophages, and reduced edema and the amount of infiltrated cells in animal models.

Conclusion: KHU14 exhibited anti-inflammatory effects as demonstrated in typical immunological tests for anti-inflammation in vitro and in vivo.

View Similar Images In: Results  - Collection
View Article: Pubmed Central - HTML -  PubMed
Show All Figures - Show MeSH
getmorefigures.php?pmc=2546402&rFormat=json&query=null&req=5