Limits...
Accelerated Neutrophil Apoptosis in Mice Lacking A1-a, a Subtype of the bcl-2–related A1 Gene

Hamasaki A, Sendo F, Nakayama K, Ishida N, Negishi I, Nakayama K, Hatakeyama S - J. Exp. Med. (1998)

Bottom Line: On the other hand, the extent of tumor necrosis factor alpha-induced acceleration of neutrophil apoptosis did not differ among A1-a-/-, A1-a+/-, and wild-type mice.The descending order of A1 mRNA expression was wild-type, A1-a+/-, and A1-a-/-.Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.

Affiliation: Department of Immunology and Parasitology, Yamagata University School of Medicine, Yamagata 990-9585, Japan.

ABSTRACT

To elucidate the role of A1, a new member of the Bcl-2 family of apoptosis regulators active in hematopoietic cell apoptosis, we established mice lacking A1-a, a subtype of the A1 gene in mice (A1-a-/- mice). Spontaneous apoptosis of peripheral blood neutrophils of A1-a-/- mice was enhanced compared with that of either wild-type mice or heterozygous mutants (A1-a+/- mice). Neutrophil apoptosis inhibition induced by lipopolysaccharide treatment in vitro or transendothelial migration in vivo observed in wild-type mice was abolished in both A1-a-/- and A1-a+/- animals. On the other hand, the extent of tumor necrosis factor alpha-induced acceleration of neutrophil apoptosis did not differ among A1-a-/-, A1-a+/-, and wild-type mice. The descending order of A1 mRNA expression was wild-type, A1-a+/-, and A1-a-/-. Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.

Representative microscopic images of normal and apoptotic  neutrophils. Aliquots (0.4 ml) of PBN suspensions of A1-a−/− mice (0.5 ×  106/ml) were incubated at 37°C for 12 h in RPMI 1640 medium. Arrows  indicate normal neutrophils and arrowhead indicates an apoptotic neutrophil containing condensed and fragmented nuclei (original magnification,  ×1,000, May-Giemsa stain).
© Copyright Policy

Figure 1: Representative microscopic images of normal and apoptotic neutrophils. Aliquots (0.4 ml) of PBN suspensions of A1-a−/− mice (0.5 × 106/ml) were incubated at 37°C for 12 h in RPMI 1640 medium. Arrows indicate normal neutrophils and arrowhead indicates an apoptotic neutrophil containing condensed and fragmented nuclei (original magnification, ×1,000, May-Giemsa stain).

Mentions: Neutrophils incubated under specific conditions were stained with May-Giemsa and apoptotic cells were counted under light microscopy (×1,000). Representative microscopic images of normal and apoptotic neutrophils are shown in Fig. 1. Approximately 300–1200 cells were scanned per specimen.

View Similar Images In: Results  - Collection
View Article: PubMed Central -  PubMed
Show All Figures - Show MeSH
getmorefigures.php?pmc=2212378&rFormat=json&query=null&req=5
Accelerated Neutrophil Apoptosis in Mice Lacking A1-a, a Subtype of the bcl-2–related A1 Gene

Hamasaki A, Sendo F, Nakayama K, Ishida N, Negishi I, Nakayama K, Hatakeyama S - J. Exp. Med. (1998)

Representative microscopic images of normal and apoptotic  neutrophils. Aliquots (0.4 ml) of PBN suspensions of A1-a−/− mice (0.5 ×  106/ml) were incubated at 37°C for 12 h in RPMI 1640 medium. Arrows  indicate normal neutrophils and arrowhead indicates an apoptotic neutrophil containing condensed and fragmented nuclei (original magnification,  ×1,000, May-Giemsa stain).
© Copyright Policy
Figure 1: Representative microscopic images of normal and apoptotic neutrophils. Aliquots (0.4 ml) of PBN suspensions of A1-a−/− mice (0.5 × 106/ml) were incubated at 37°C for 12 h in RPMI 1640 medium. Arrows indicate normal neutrophils and arrowhead indicates an apoptotic neutrophil containing condensed and fragmented nuclei (original magnification, ×1,000, May-Giemsa stain).
Mentions: Neutrophils incubated under specific conditions were stained with May-Giemsa and apoptotic cells were counted under light microscopy (×1,000). Representative microscopic images of normal and apoptotic neutrophils are shown in Fig. 1. Approximately 300–1200 cells were scanned per specimen.

Bottom Line: On the other hand, the extent of tumor necrosis factor alpha-induced acceleration of neutrophil apoptosis did not differ among A1-a-/-, A1-a+/-, and wild-type mice.The descending order of A1 mRNA expression was wild-type, A1-a+/-, and A1-a-/-.Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.

Affiliation: Department of Immunology and Parasitology, Yamagata University School of Medicine, Yamagata 990-9585, Japan.

ABSTRACT

To elucidate the role of A1, a new member of the Bcl-2 family of apoptosis regulators active in hematopoietic cell apoptosis, we established mice lacking A1-a, a subtype of the A1 gene in mice (A1-a-/- mice). Spontaneous apoptosis of peripheral blood neutrophils of A1-a-/- mice was enhanced compared with that of either wild-type mice or heterozygous mutants (A1-a+/- mice). Neutrophil apoptosis inhibition induced by lipopolysaccharide treatment in vitro or transendothelial migration in vivo observed in wild-type mice was abolished in both A1-a-/- and A1-a+/- animals. On the other hand, the extent of tumor necrosis factor alpha-induced acceleration of neutrophil apoptosis did not differ among A1-a-/-, A1-a+/-, and wild-type mice. The descending order of A1 mRNA expression was wild-type, A1-a+/-, and A1-a-/-. Taken together, these results suggest that A1 is involved in inhibition of certain types of neutrophil apoptosis.

View Similar Images In: Results  - Collection
View Article: PubMed Central -  PubMed
Show All Figures - Show MeSH
getmorefigures.php?pmc=2212378&rFormat=json&query=null&req=5